1778 pLVX-Flu A/WSN/33 M15/88K-TagGFP
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Cloning of1778 pLVX-Flu A/WSN/33 M15/88K-TagGFP
[edit]Purpose: Cloning of 1778 pLVX-Flu A/WSN/33 M15/88K-TagGFP
Date: 2013-7--2-
Notebook:05846
Page:
Clone ID:1778
Experimenters: Shanyi
Results
[edit]cloning
[edit]PCR use 1702 pET30b-A/WSN/33 M15/88K as template
digest PCR products with Xho1 + BamH1, then ligate to Xho1 + BamH1 digested 46 pLVX-TagGFP-2
Colony Screening
[edit]Date: 2013-7-
Make miniprep and digest with Xho1 + BamH1