1788 pT7A31-3C-(tev)-ELP
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Cloning of 1788 pT7A31-3C-(tev)-ELP
[edit]Purpose: Cloning of 1788 pT7A31-3C-(tev)-ELP
Date: 07,26, 2013
Notebook:
Page:
Clone ID: 1788
Experimenters: Shanyi
Results
[edit]Cloning
[edit]PCR from 1420 pDB.His.MBP.3C using these primers:6H-3C-Nde1-F and 3C-(TEV)-Sal1-R
digest
PCR: Nde1 + Sal1
Vector: Nde1 + Xho1
Colony screen
[edit]digest with