1793-pET28b-VPol1

Cloning of 1793-pET28b-VPol1

Purpose: Cloning of 1793-pET28b-VPol1

Date: 2013-8-1----2013-8-7

Notebook:0

Page:

Clone ID:1793

Experimenters: yingying

Results

cloning

gene synthesis

VPol1-1-1  to VPol1-1-18

VPol1-2-1 to VPol1-1-2-14

VPol1-3-1 to VPol1-3-16

template:pcr1+pcr2+pcr3

VPol1-1-1+VPol1-3-16

lane	3	4
	PCR product	1074,pET28b-IHFab
enzymes	Nco1 + Xho1

Colony Screening

Date: 2013-8-7

Make miniprep and digest with Nco1 + Xho1

Fixing of Cloning of 1793-pET28b-VPol1

Purpose: fixing of Cloning of 1793-pET28b-VPol1

Date: 2013-8-14----2013-8-16

Notebook:0

Page:

Clone ID:1793

Experimenters: yingying

Results

cloning

Template: 1793-2 (2013/8/7)

PCR1:1-1 to 2-4

PCR2:2-5 to 3-16

template:pcr1+pcr2

VPol1-1-1+VPol1-3-16

lane	1	2
	PCR product	1074,pET28b-IHFab
enzymes	Nco1 + Xho1

Colony Screening

Date: 2013-8-16

Make miniprep and digest with Nco1 + Xho1

Conclusion

2013-8-30