1798-pCold-6His-MCS-(TEV-3C)-MBP

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Cloning of 1798-pCold-6His-MCS-(TEV-3C)-MBP

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Purpose: Cloning of 1798-pCold-6His-MCS-(TEV-3C)-MBP

Date: 2013-7-22---2013-7-25

Notebook:0

Page:

Clone ID:1798

Experimenters: yingying

Results

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cloning

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PCR MBP using 1281 pMAL-p5E as template
MBP-3C-HindIII-F
MBP-Sal1-stop-R2


lane
 1
 2

 PCR product
 v19 pET28-cold
enzymes
 HindIII + Sal1
 HindIII + Xho1


Colony Screening

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Date: 2013-7-25

Make miniprep and digest with HindIII + Bgl11



Result intepretation/Summary

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Conclusion

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