1803-pCold-6His-GST-(E)-(3C)-PKARIIb

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Cloning of 1803-pCold-6His-GST-(E)-(3C)-PKARIIb

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Purpose: Cloning of 1803-pCold-6His-GST-(E)-(3C)-PKARIIb

Date: 2013-7-30---2013-8-2

Notebook:0

Page:

Clone ID:1803

Experimenters: yingying

Results

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cloning

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lane
 1
 2

 1781 pTCold-MBP-(3C-TEV)-PKARIIb
 1624 pCold-6His-GST-(E)-(3C)
enzymes
               Nde1 + Xho1


Colony Screening

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Date: 2013-8-2

Make miniprep and digest with Nde1 + Xho1



Result intepretation/Summary

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Conclusion

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