1809-pCold-MBP-(E3C)-FN3K

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Cloning of 1809-pCold-MBP-(E3C)-FN3K

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Purpose: Cloning of 1809-pCold-MBP-(E3C)-FN3K

Date: 2013-8-2---2013-8-6

Notebook:0

Page:

Clone ID:1809

Experimenters: yingying

Results

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cloning

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PCR from DNA isolated from
Harvard Kinase Plate HRG007900, 79
Well: G10

FN3K-Nde1-F
FN3K-Xho1-R



lane
 1
 2

 PCR product
 1804, pCold-MBP-(E3C)
enzymes
              Nde1 + Xho1



Colony Screening

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Date: 2013-8-6

Make miniprep and digest with Nde1 + Xho1/AlwN1



Result intepretation/Summary

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Conclusion

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