1812-pET28b-HTNFlp-Ecoli
Cloning of 1812-pET28b-HTNFlp-Ecoli
[edit]Purpose: Cloning of 1812-pET28b-HTNFlp-Ecoli
Date: 2013-8-9------2013-8-16
Notebook:0
Page:
Clone ID:1812
Experimenters: yingying
Results
[edit]cloning
[edit]
gene synthesis
pcr1:Ecoli-Flpe-1-1 to Ecoli-Flpe-1-18
pcr2:Ecoli-Flpe-2-1 to Ecoli-Flpe-2-18
pcr1+pcr2
PCR:Ecoli-Flpe-1-1 to Ecoli-Flpe-2-18
| lane |
3 |
4 |
| PCR product |
1078,pET28b-scIHF2 | |
| enzymes |
Nco1 + Xho1 | |
Colony Screening
[edit]Date: 2013-8-16
Make miniprep and digest with Nco1 + Xho1
Result intepretation/Summary
[edit]Conclusion
[edit]
Fix of Cloning of 1812-pET28b-HTNFlp-Ecoli
[edit]Purpose: Cloning of 1812-pET28b-HTNFlp-Ecoli
Date: 2013-8-20----2013-8-28
Notebook:0
Page:
Clone ID:1812
Experimenters: yingying
Results
[edit]cloning
[edit]
gene synthesis
pcr1:Ecoli-Flpe-1-1 to Ecoli-Flpe-2-6
pcr2:Ecoli-Flpe-2-7 to Ecoli-Flpe-2-18
pcr1+pcr2
PCR:Ecoli-Flpe-1-1 to Ecoli-Flpe-2-18
| lane |
1 |
3 |
| PCR product |
1078,pET28b-scIHF2 | |
| enzymes |
Nco1 + Xho1 | |
Colony Screening
[edit]Date: 2013-8-28
Make miniprep and digest with Nco1 + Xho1
Result intepretation/Summary
[edit]Conclusion
[edit]final fix
2013-8-30
FIX one A missing
2013-9-6---2013-9-9
