1816 pCold-MBP-(E3C)-Enterokinase
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Cloning of 1816 pCold-MBP-(E3C)-Enterokinase
[edit]Purpose: Cloning of1816 pCold-MBP-(E3C)-Enterokinase
Date: 08, 06, 2013
Notebook:
Page:
Clone ID: 1816
Experimenters: Shanyi
Results
[edit]Cloning
[edit]subclone from 1814 pMAL-p5E-Enterokinase or 1815 pMAL-c5E-Enterokinase to 1804 pCold-MBP-(E3C) using Bsa1 + BamH1
cut 1814 the middle band=>2000bp
should be 1884 bp
Colony screen
[edit]8/10/2013: make miniprep, digest with Kpn1 to screen, clone #5 could be the desired clone
[edit]
[edit]
8/112013: digest clone #5 with enzymes to verify
[edit]
lane 2: should be Nco1 + Xho1, not Nde1 + Xho1
