1817-pCold-MBP-(E3C)-MCSK

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Cloning of 1817-pCold-MBP-(E3C)-MCSK

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Purpose: Cloning of 1817-pCold-MBP-(E3C)-MCSK

Date: 2013-8-7---2013-8-9

Notebook:0

Page:

Clone ID:1817

Experimenters: yingying

Results

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cloning

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lane
 1 vector

 1804,pCold-MBP-(E3C)
enzyme
 Nde1 + Xho1

ligate to annealed oligos Nde1-MCS-Xho1-T2 + Nde1-MCS-Xho1-B2


Colony Screening

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Date: 2013-8-9

Make miniprep and digest with Sal1 + BglII



Result intepretation/Summary

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Conclusion

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