1825-pCold-MBP-(a3C)-NRF2-pLacZ-3C
Jump to navigation
Jump to search
Cloning of 1825-pCold-MBP-(a3C)-NRF2-pLacZ-3C
[edit]Purpose: Cloning of 1825-pCold-MBP-(a3C)-NRF2-pLacZ-3C
Date: 2013-8-19----2013-8-21
Notebook:0
Page:
Clone ID:1825
Experimenters: yingying
Results
[edit]cloning
[edit]
| lane |
3 |
4 |
| 1796, pT7A3-MBP-(3C-TEV)-NRF2-6His |
1806 pCold-MBP-(a3C)-MCS-pLacZ-3C | |
| enzymes |
Nde1 + Xho1 | |
Colony Screening
[edit]Date: 2013-8-21
Make miniprep and digest with Nde1 + Xho1
clone3 for stock
Result intepretation/Summary
[edit]Conclusion
[edit]