1943-pT7A3-T7-RNAPol-Y639F/H784A

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Cloning of 1943-pT7A3-T7-RNAPol-Y639F/H784A

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Purpose: Cloning of 1943-pT7A3-T7-RNAPol-Y639F/H784A

Date: 2013-12-30---2014-1-2

Notebook:0

Page:

Clone ID:1943

Experimenters: yingying

Results

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cloning

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pcr1 using T7 Phage genomic DNA as template
T7-RNAPol-Nhe1-F
T7RNAPol-Y639F-R

pcr2 using T7 Phage genomic DNA as template

T7RNAPol-Y639F-F
T7-RNAPol-H784A-R

pcr3 using T7 Phage genomic DNA as template

T7-RNAPol-H784A-F
T7RNAPol-Xho1-R2


pcr1+pcr2+pcr3

LANE
 3
 4

 PCR
1892 pT7A3E

 Nhe1 + Xho1


Colony Screening

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Date: 2014-1-2

Make miniprep and digest with Nhe1 + Xho1


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Result intepretation/Summary

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Conclusion

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