2185-pCold-MBP-(Ent-TEV)

Cloning of 2185-pCold-MBP-(Ent-TEV)

Purpose: Cloning of 2185-pCold-MBP-(Ent-TEV)

Date: 2014-5-2---2014-5-5

Notebook:0

Page:

Clone ID:2185

Experimenters: yingying

Results

cloning

PCR from 1804 pCold-MBP-(E3C)

1. Step 1
Fragment 1
Cold-promoter-Nhe1-F
Cold-pomoter-MBP-R

Fragment 2

Cold-pomoter-MBP-F
MBP-TEV-Nco1-Nde1-R

PCR:pcr1+pcr2

	PCR	1804 pCold-MBP-(E3C)
	Nhe1 + Nde1

Colony Screening

Date: 2014-5-5

Make miniprep and digest with Nhe1 + Nco1

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