2381 2383 2386 expression screening 2375 purification
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Protein Expression
[edit]2014/11/24
SHANYI
Nico21(DE3)plysN 0.2mM IPTG RT induce overnight
| MW, PI | Expression | ||||
| 1 | 2381 |
pT7A3-TRX-Tat-NLS-iFlp-6H |
9.32 / 66.1 |
supernatant |
|
| 2 | 2381 |
pT7A3-TRX-Tat-NLS-iFlp-6H |
9.32 / 66.1 |
pellet |
Y |
| 3 | 2383 |
pT7A3-6XHis-SMT3-Tat-NLS-iFlp-6H |
9.49 / 65.0 |
supernatant |
|
| 4 | 2383 |
pT7A3-6XHis-SMT3-Tat-NLS-iFlp-6H |
9.49 / 65.0 |
pellet |
Y |
| 5 | 2386 |
pET30B-T4-uvsX |
supernatant |
||
| 6 | 2386 |
pET30B-T4-uvsX |
pellet |
||
| 7 | PageRuler Plus |
||||
| 8 | 2375 |
pT7A3-MBP-TRX-6H |
supernatant |
||
| 9 | 2375 |
pT7A3-MBP-TRX-6H |
supernatant |
||
| 10 | 2375 |
pT7A3-MBP-TRX-6H |
pellet |
Y | |
| 11 | 2375 |
pT7A3-MBP-TRX-6H |
pellet |
Y | |
| 12 | 2375 |
pT7A3-MBP-TRX-6H |
pellet |
Y |