2382 pT7A3-6XHis-SMT3-(Tev)
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Cloning of2382 pT7A3-6XHis-SMT3-(Tev)
[edit]Purpose: Cloning of2382 pT7A3-6XHis-SMT3-(Tev)==
Date: 2014-11----
Notebook:0
Page:
Clone ID:2382
Experimenters: shanyi
Results
[edit]CLONING
[edit]CR from 2285 pBY011-SMT3
2197-SUMO-Nco1-F1 tcgac cc atg ggc agc agc cac cat cac cat cac cat tcggactcagaagtcaatc
2382-SMT3_TEV_Nde1-R (cacagagaacagattggtggt ggggatgacgatgacaaggaaaacctttacttccagggccatatg cat atg tagcac) gtgctacatatgcatatggccctggaagtaaaggttttccttgtcatcgtcatccccaccaccaatctgttctctgtg
Digest with Nde1-Nco1
Colony Screening
[edit]Date: 2014_11
Make miniprep, use Nde1-Nco1 to find positive clone
