3233 pCMV5-IL2-CD47-(3c)-Fc-WPRE-OriP

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3233 subclone_3194 vector digestion

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Miniprep of 3233_3199_2509

Digest with Nhe1-KpnI 

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Additional QC verification: digest 3233 clone 1 and 2 with EcoR1 + Kpn1, using Nhe1 + Kpn1 as control.

make glycerol stock for  3233 clone 1 and 2, also label plasmid DNA

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