3234 pCMV5-IL2-PD-1-(3c)-Fc-WPRE-OriP

Cloning of

Purpose:  

Date: 2018-6-22

Notebook: 

Page:

Clone ID:3237

Experimenters: Paul 

Results

PCR

Date: Date: 6/21/2018

By: Paul

template:

primers:

Gel:

http://www.excellgen.net/eln/images/5/52/3237_3232_3234_PCR.JPG

Cloning

By: Paul

Vector:  

Ligation: 30 min with T4 DNA ligase

Competent cells: NEBTurbo

Colony PCR Screening 

~ 

Restriction Digestion

Date: 6/23/2018

By: Paul

Sequencing