664-pEC-Eag1
(Redirected from 664-pET28b-Eag1)
Cloning of 664-pEC-Eag1
[edit]Purpose: Cloning of 664-pEC-Eag1
Date: 2013-9-3---2013-9-6
Notebook:0
Page:
Clone ID:664
Experimenters: yingying
Results
[edit]cloning
[edit]
pcr1:Ega1-1-1 to Ega1-1-12
pcr2:Ega1-2-1 to Ega1-2-12
PCR: pcr1+pcr2
Ega1-1-1 to Ega1-2-12
| lane |
1 |
2 |
| PCR product |
1454,pEC | |
| enzymes |
Nde1 + Xho1 | |
Colony Screening
[edit]Date: 2013-9-9
Make miniprep and digest with Nde1 + Xho1
fix clone1
2013-9-13
Nde1 + BamH1
Result intepretation/Summary
[edit]Conclusion
[edit]