File:382 TagGFP PCR 9 15 2011.JPG
382_TagGFP_PCR_9_15_2011.JPG (189 × 200 pixels, file size: 28 KB, MIME type: image/jpeg)
Cloning of 382 pCMVK-IRES-TagGFP
Purpose: To clone 382 pCMVK-IRES-TagGFP by PCR
Date: 2011-9-14---
Notebook:Lu yingying
Page:003896---
Clone ID:382
Experimenters: lu yingying
Results:
All bands show PCR product of IRES-TagGFP,
Primer-F 8685-TagGFP-F Xho1 HindIII 22nt IRES Kozak TagGFP atcg CTCGAG AAGCTT TTTATAATTTCTTCTTCCAGAA GCCACC ATG AGC GGG GGC GAG
Primer-R 8685-TagGFP-R BglII (CATGGACGAGCTGTACAGG taa AGATCT atgct) agcatAGATCTttaCCTGTACAGCTCGTCCATG
Digest result, Xho1+BglII
band 1,2,3 PCMVK vector,band 4,5.6 IRES-TagGFP
Gene Synthesis
Colony Screening
Result intepretation/Summary
File history
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 15:30, 16 September 2011 | | 189 × 200 (28 KB) | Luyingying (talk | contribs) | |
| 09:08, 16 September 2011 |  | 189 × 200 (28 KB) | Luyingying (talk | contribs) | ||
| 08:59, 16 September 2011 |  | 189 × 200 (26 KB) | Luyingying (talk | contribs) |
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