MBP,MMLV,UDG Protein Screening

protein expression screening

360: pDB.His.MBP

1428: PT7-MBP

1429: pT7A3-MBP

501: MMLV

1034: UDG

Data:2012-11-2--2012-11-7

Exprimenter: yingying lu

3 ml sample each ,then 0.2mMIPTG induce 37C for 5 hours

picture1: supernatant

Clone ID	360		1428		1429		501		1034
Name	pDB.His.MBP		PT7-MBP		pT7A3-MBP		MMLV		UDG
Lane	1	2	3	4	5	6	7	8	9	10
IPTG mM	0	0.2	0	0.2	0	0.2	0	0.2	0	0.2

Picture2: pellet

Clone ID	360		1428		1429		501		1034
Lane	1	2	3	4	5	6	7	8	9	10
IPTG mM	0	0.2	0	0.2	0	0.2	0	0.2	0	0.2

conclusion: 360 and 1429 express MBP protein; 1034 express UDG in pellet; 1428 has no MBP expression; 501 has no MMLV expression