Signal peptide comparison
Transfection:
5 ul (100 ~ 200 ng/ul) from miniprep, each well is from individuale clone
24 well plate.
1393: pCMV5-IL2-TagGFP-(3c)-Fc-WPRE-OriP
1394: pCMV5-CST4-TagGFP-Fc(3c)-WPRE-OriP
1395: pCMV5-hIgG-Lc-TagGFP-Fc(3c)-WPRE-OriP
1396: pCMV5-hIgG-Hc-TagGFP-Fc(3c)-WPRE-OriP
Nov 2012
Transfect with L2K. 1433 plasmid DNA quality is low, only 20 ng/ul
2012/11/23
11/24/2012
11/20/2012: Transfection using lipofectamine 2000, 1 ug DNA, 3 ul L2K, 12 well plate
Picture on 2012/11/22
Picture on 2012/11/23
11/24/2012
2012/11/25
Signal_seq_comparison_2013_1_24.jpg
11/21/2012: Transfection using GeneExpression Max
1 ug DNA, 3 ul GEM, 12 well plate
Picture on 11/22/2012
2012/11/24
2012/11/25
2012/12/12
transfection on 2012/12/11: 1 ug/well, 3 ul GEM, picture taken on 2012/12/12
2012/12/13
2012/12/14
plate
Compare new IL2 signal peptide vs other
[edit]12 well plate, 293T cells
Transfection: 1 ug DNA
Transfection reagent: GEM
Date: 2013/1/24
2013 1 25
2013/1/26
