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== Tag Antibodies == {| border="1" cellspacing="1" cellpadding="1" style="width: 721px;" |- | style="width: 100px;" | Urgency | style="width: 100px;" | Name | style="width: 26px;" | Chain | style="width: 47px;" | Clone ID | style="width: 171px;" | Name | style="width: 194px;" | Parent | style="width: 194px;" | Vector | style="width: 49px;" | Insert Size | style="width: 345px;" | Enzymes | style="width: 345px;" | Status/note |- | style="width: 100px;" | 0 | style="width: 100px;" | '''Vector'''<br/> | style="width: 26px;" | L | style="width: 47px;" | 3154<br/> | style="width: 171px;" | pCMV5-HA-VH-mKappa<br/> | style="width: 194px;" | HA 3129 pMiniT2-3129-1<br/> | style="width: 194px;" | 2493 pCMV5-mIgG-kLC<br/> | style="width: 49px;" | 527 | style="width: 345px;" | <span style="color: rgb(0, 0, 0); font-family: Arial, Verdana, sans-serif; font-size: 12px; background-color: rgb(251, 251, 239);">BamH1-Kpn1</span><br/> | style="width: 345px;" | <span style="color:#FF0000;">sequenced, good, glycerol stock made</span> |- | style="width: 100px;" | <s>3</s> | style="width: 100px;" | <br/> | style="width: 26px;" | <s>H</s> | style="width: 47px;" | <s>3129 </s><br/> | style="width: 171px;" | <s>pCMV5-HA-VH-mIgG2b</s><br/> | style="width: 194px;" | <s>new 3129 pMiniT with b</s> | style="width: 194px;" | <s>3154 pCMV5-Flag-H</s><br/> | style="width: 49px;" | <s>1034</s><br/> | style="width: 345px;" | <s>Kpn1 + Not1</s><br/> | style="width: 345px;" | <s>3129-BamH1-F + 3129-Kpn1-F1</s><br/> |- | style="width: 100px;" | -<s>2</s> | style="width: 100px;" | <br/> | style="width: 26px;" | <s>L</s> | style="width: 47px;" | <s>3021</s> | style="width: 171px;" | <s>pCEL0-mIg-LCKappa</s><br/> | style="width: 194px;" | <br/> | style="width: 194px;" | <s><span style="color:#FF0000;">3016 pCEL0</span></s><br/> | style="width: 49px;" | <br/> | style="width: 345px;" | <s>Vector <span style="color: rgb(255, 0, 0);">3016 pCEL0</span>: BssHII - Nhe1<br/>insert in pMiniT2: BssHII - Xba1</s><br/> | style="width: 345px;" | <s>PCR from IgG kappa LC from 2493 pCMV5-mIgG-kLC, 3021-F2 + 3021-Age1-Xba1-R , Vector: BssHII - Nhe1, insert: BssHII - Xba1, in <span style="color:#FF0000;"> pMiniT2 vector now.</span></s><br/> |- | style="width: 100px;" | -<s>2</s> | style="width: 100px;" | <br/> | style="width: 26px;" | <s>H</s> | style="width: 47px;" | <s>3027 </s><br/> | style="width: 171px;" | <s>pCEH0-mIgG2b-HC</s><br/> | style="width: 194px;" | <s>3076-pMiniT2</s><br/> | style="width: 194px;" | <s><span style="color: rgb(0, 0, 0); font-family: Arial, Verdana, sans-serif; font-size: 12px;">3017 pCEH0</span></s><br/> | style="width: 49px;" | <br/> | style="width: 345px;" | <s>Nhe1-Not1</s><br/> | style="width: 345px;" | <s>PCR from 2492 pCMV5-mIgG2b-HC using 3020-Xba1-Nhe1-F + 3027-2b-Not1-Age1-R</s> <s><span style="color:#FF0000;">in pMiniT2 now, sequenced</span></s> |- | style="width: 100px;" | 0 | style="width: 100px;" | '''His''' | style="width: 26px;" | H+L, sc | style="width: 47px;" | 3002<br/> | style="width: 171px;" | pJet1.2-Anti-His Tag 3D5<br/> | style="width: 194px;" | <br/> | style="width: 194px;" | pJet1.2<br/> | style="width: 49px;" | <br/> | style="width: 345px;" | Nde1-Sal1/Xho1 | style="width: 345px;" | 3129-Kpn1-F1, sequenced, <span style="color:#FF0000;">good, </span>need to subclone into <span style="color:#FF0000;">pET30b</span>, but there is no tag for purification<br/> |- | style="width: 100px;" | 0 | style="width: 100px;" | '''His ''' | style="width: 26px;" | L | style="width: 47px;" | 3001 <br/> | style="width: 171px;" | pMiniT2-His-VL <br/> | style="width: 194px;" | <br/> | style="width: 194px;" | <br/> | style="width: 49px;" | <br/> | style="width: 345px;" | <br/> | style="width: 345px;" | <span style="color:#FF0000;">good, sequenced</span> |- | style="width: 100px;" | 0 | style="width: 100px;" | <br/> | style="width: 26px;" | H<br/> | style="width: 47px;" | 3135<br/> | style="width: 171px;" | 3135-3-pMiniT2<br/> | style="width: 194px;" | <br/> | style="width: 194px;" | pMiniT2<br/> | style="width: 49px;" | <br/> | style="width: 345px;" | <br/> | style="width: 345px;" | in pMiniT2 vector now, 3135-3-pMiniT2 sequenced, <span style="color:#FF0000;">good, </span>need to subclone into 3129 pCMV5-HA-VH-mIgG2b using <span style="color:#FF0000;">BsrG1-Kpn1</span> |- | style="width: 100px;" | 0 | style="width: 100px;" | <br/> | style="width: 26px;" | L | style="width: 47px;" | 3001 <br/> | style="width: 171px;" | pCMV5-His-VL-kappa<br/> | style="width: 194px;" | <span style="color: rgb(255, 0, 0);">3001-pMiniT2</span><br/> | style="width: 194px;" | 3130 pCMV5-HA-VL-mIgKappa<br/> | style="width: 49px;" | 435 | style="width: 345px;" | BamH1-Kpn1 | style="width: 345px;" | <span style="color:#FF0000;">3001-pMiniT2 is good, sequenced</span> subclone from 3001-pMiniT2, |- | style="width: 100px;" | -2 | style="width: 100px;" | <br/> | style="width: 26px;" | H | style="width: 47px;" | 3135 | style="width: 171px;" | pCMV5-His-VH-IgG2b<br/> | style="width: 194px;" | 3135-3-pMiniT2 <br/> | style="width: 194px;" | 3129 pCMV5-HA-VH-mIgG2b <s>3009pCMV5-Flag-VH-IgG2b</s> | style="width: 49px;" | 364 | style="width: 345px;" | BsrG1-Kpn1 <br/> | style="width: 345px;" | <span style="color:#FF0000;">subclone </span>into 3129 pCMV5-HA-VH-mIgG2b using BsrG1-Kpn1 from 3135-3-pMiniT2 <br/> |- | style="width: 100px;" | 0 | style="width: 100px;" | <br/> | style="width: 26px;" | H | style="width: 47px;" | 3168<br/> | style="width: 171px;" | pCEH0-His-VH-mIgG2a<br/> | style="width: 194px;" | 3135-3-pMiniT2 <br/> | style="width: 194px;" | 3163 pCEH0-1FRG-HA-VH-mIgG2a<br/> | style="width: 49px;" | 358 | style="width: 345px;" | BsrG1-Nhe1<br/> | style="width: 345px;" | 3135-3-pMiniT2 VHSQVQLQQSGPEDVKPGASVKISCKASGYTFTDYYMNWVKQSPGKGLEWIGDINPNNGGTSYNQKFKGRATLTVDKSSSTAYMELRSLTSEDSSVYYCESQSGAYWGQGTTVTVSAASS |- | style="width: 100px;" | 0 | style="width: 100px;" | Myc<br/> | style="width: 26px;" | L | style="width: 47px;" | 3003 <br/> | style="width: 171px;" | 3003-L-5<br/> | style="width: 194px;" | <br/> | style="width: 194px;" | pJet1.2<br/> | style="width: 49px;" | <br/> | style="width: 345px;" | <br/> | style="width: 345px;" | IL2 signal peptide, BamH1-Kpn1, good<br/> |- | style="width: 100px;" | 0 | style="width: 100px;" | <br/> | style="width: 26px;" | H | style="width: 47px;" | 3003 | style="width: 171px;" | 3003-H-1<br/> | style="width: 194px;" | <br/> | style="width: 194px;" | pJet1.2<br/> | style="width: 49px;" | <br/> | style="width: 345px;" | <br/> | style="width: 345px;" | need to use 3076-myc-BsrG1-F to <span style="color:#FF0000;">fix </span>frame shift |- | style="width: 100px;" | 0 | style="width: 100px;" | <br/> | style="width: 26px;" | L | style="width: 47px;" | 3075<br/> | style="width: 171px;" | pCMV5-Myc-L-kappa<br/> | style="width: 194px;" | <br/> | style="width: 194px;" | 2493 pCMV5-mIgG-kLC<br/> | style="width: 49px;" | <br/> | style="width: 345px;" | <br/> | style="width: 345px;" | <span style="color:#FF0000;">completed, </span>subclone from 3003-L5 using BamH1-Kpn1 to 2493 pCMV5-mIgG-kLC <span style="color:#FF0000;">sequenced, good</span> |- | style="width: 100px;" | -2 | style="width: 100px;" | <br/> | style="width: 26px;" | H | style="width: 47px;" | 3076<br/> | style="width: 171px;" | pCMV5-Myc-H-IgG2b<br/> | style="width: 194px;" | <br/> | style="width: 194px;" | 3129 pCMV5-HA-VH-mIgG2b | style="width: 49px;" | <br/> | style="width: 345px;" | <span style="color: rgb(255, 0, 0);"> </span>BsrG1-Kpn1 <br/> | style="width: 345px;" | PCR from 3003-H-1 in pJet1.2, using 3076-myc-BsrG1-F and 3076-myc-Nhe1-Kpn1-R <span style="color:#FF0000;">in pMiniT2 now sequenced, good</span> |- | style="width: 100px;" | 0 | style="width: 100px;" | <br/> | style="width: 26px;" | H | style="width: 47px;" | <span style="color:#FF0000;">3167</span><br/> | style="width: 171px;" | pCEH0-Myc-VH-mIgG2a<br/> | style="width: 194px;" | 3027-pMiniT2 (3076 myc pMiniT2) | style="width: 194px;" | <span style="color:#FF0000;">3163 </span>pCEH0-1FRG-HA-VH-mIgG2a<br/> | style="width: 49px;" | 397 | style="width: 345px;" | BsrG1-Nhe1 | style="width: 345px;" | subcloning from the misslabeled myc clone in pMiniT2 EVDLVESGGDLVKPGGSLKLSCAASGFTFSHYGMSWVRQTPDKRLEWVATIGSRGTYTHYPDSVKGRFTISRDNDKNALYLQMNSLKSEDTAMYYCARRSEFYYYGNTYYYSAMDYWGQGTTVTVSS |- | style="width: 100px;" | 0 | style="width: 100px;" | HA, <span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px;">1IFH</span><br/> | style="width: 26px;" | sc VH+VL | style="width: 47px;" | 3074 <br/> | style="width: 171px;" | pT7A30-HA-Avi-BirA<br/> | style="width: 194px;" | <br/> | style="width: 194px;" | pJet1.2 | style="width: 49px;" | <br/> | style="width: 345px;" | <br/> | style="width: 345px;" | 3074-1-PJET1-2 is <span style="color:#FF0000;">good,</span> need to subclone into 1834 pT7A30-Avi-BirA<br/> |- | style="width: 100px;" | 0 | style="width: 100px;" | HA, <span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px;">1IFH</span><br/> | style="width: 26px;" | L | style="width: 47px;" | 3011<br/> | style="width: 171px;" | pJet1.2-HA-L-1<br/> | style="width: 194px;" | <br/> | style="width: 194px;" | pJet1.2<br/> | style="width: 49px;" | <br/> | style="width: 345px;" | <br/> | style="width: 345px;" | 3011-L-1 is <span style="color:#FF0000;">good, sequenced</span><br/> |- | style="width: 100px;" | 0 | style="width: 100px;" | HA, <span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px;">1IFH</span><br/> | style="width: 26px;" | H | style="width: 47px;" | 3011<br/> | style="width: 171px;" | pJet1.2-HA-H-2<br/> | style="width: 194px;" | <br/> | style="width: 194px;" | pJet1.2<br/> | style="width: 49px;" | <br/> | style="width: 345px;" | <br/> | style="width: 345px;" | 3011-H-2 is <span style="color:#FF0000;">good, sequenced</span> |- | style="width: 100px;" | 0 | style="width: 100px;" | HA, <span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px;">1IFH</span><br/> | style="width: 26px;" | L | style="width: 47px;" | 3030 | style="width: 171px;" | 3130-4-pMiniT2 <br/> | style="width: 194px;" | <br/> | style="width: 194px;" | pMiniT2<br/> | style="width: 49px;" | <br/> | style="width: 345px;" | <br/> | style="width: 345px;" | 3130-4-pMiniT2 is <span style="color:#FF0000;">good (sequenced)</span>, derived from 3011-L-1 pJet1.2-HA light chain by PCR using 3130-BamH1-R + 3130-Kpn1-R<br/> |- | style="width: 100px;" | 0 | style="width: 100px;" | HA, <span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px;">1IFH</span><br/> | style="width: 26px;" | L | style="width: 47px;" | 3130<br/> | style="width: 171px;" | pCMV5-HA-VL-mIgKappa<br/> | style="width: 194px;" | 3130-4-pMiniT2 <br/> | style="width: 194px;" | 2493 pCMV5-mIgG-kLC<br/> | style="width: 49px;" | <span style="color: rgb(255, 0, 0);">429bp</span><br/> | style="width: 345px;" | <span style="color: rgb(255, 0, 0);">subclone </span>from using BamH1+ Kpn1<br/> | style="width: 345px;" | <span style="color:#FF0000;">completed,good, sequenced, need to make glycerol stock</span> |- | style="width: 100px;" | 0 | style="width: 100px;" | HA, <span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px;">1IFH</span><br/> | style="width: 26px;" | H | style="width: 47px;" | 3129 | style="width: 171px;" | pMiniT2-3129-1 | style="width: 194px;" | <span style="color:#FF0000;">3011 pJet1.2-HA Heavy chain</span><br/> | style="width: 194px;" | pMiniT2<br/> | style="width: 49px;" | <br/> | style="width: 345px;" | PCR | style="width: 345px;" | <span style="color:#FF0000;">good sequenced</span> |- | style="width: 100px;" | 0 | style="width: 100px;" | HA, <span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px;">1IFH</span><br/> | style="width: 26px;" | H | style="width: 47px;" | 3129<br/> | style="width: 171px;" | pCMV5-HA-VH-mIgG2b<br/> | style="width: 194px;" | 2492 pCMV5-mIgG2b-HC | style="width: 194px;" | 3154 pCMV5-HA-VH-mKappa<br/> | style="width: 49px;" | | style="width: 345px;" | <span style="color: rgb(255, 0, 0);">subcloning </span>Kpn1 -Not1 | style="width: 345px;" | <font color="#ff0000"> subcloning mIgG2b constant domain to 3154, due to BamH1 site</font> |- | style="width: 100px;" | 0 | style="width: 100px;" | HA, <span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px;">1IFH</span><br/> | style="width: 26px;" | L | style="width: 47px;" | <span style="color:#FF0000;">3131 </span><br/> | style="width: 171px;" | pCEL0-HA-VL-mIgKappa<br/> | style="width: 194px;" | 3011 pJet1.2-HA-L-1 <br/> | style="width: 194px;" | 3021 pCEL0-mIg-LCKappa<br/> | style="width: 49px;" | 342<br/> | style="width: 345px;" | BssHII-Xho1<br/> | style="width: 345px;" | <span style="color:#FF0000;">subclone</span> 342 bp fragment from 3011 pJet1.2-HA-L-1 using BssHII-Xho1 <span style="color:#FF0000;">do not use the smallest fragment</span> |- | style="width: 100px;" | 0 | style="width: 100px;" | HA, <span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px;">1IFH</span><br/> | style="width: 26px;" | H | style="width: 47px;" | <span style="color:#FF0000;">3132</span> <br/> | style="width: 171px;" | pCEH0-HA-VH-mIgG2a<br/> | style="width: 194px;" | 3011 pJet1.2-HA-H-2<br/> | style="width: 194px;" | <span style="color:#FF0000;">3163</span> pCEH0-1FRG-HA-VH-mIgG2a<br/> | style="width: 49px;" | 373<br/> | style="width: 345px;" | BsrG1-Nhe1<br/> | style="width: 345px;" | <span style="color:#FF0000;">subclone</span> 373 bp fragment from 3011 pJet1.2-HA-H-2 using BsrG1-Nhe1 |- | style="width: 100px;" | 0 | style="width: 100px;" | HA, 1FRG<br/> | style="width: 26px;" | L | style="width: 47px;" | 3159<br/> | style="width: 171px;" | pMiniT2-1FRG-HA-VL-kappa<br/> | style="width: 194px;" | <br/> | style="width: 194px;" | pMiniT2 | style="width: 49px;" | <br/> | style="width: 345px;" | <br/> | style="width: 345px;" | gene fragment from IDT |- | style="width: 100px;" | 0 | style="width: 100px;" | HA, 1FRG<br/> | style="width: 26px;" | H | style="width: 47px;" | 3160 <br/> | style="width: 171px;" | pMiniT-2-1FRG-HA-VH-mIgG2a<br/> | style="width: 194px;" | <br/> | style="width: 194px;" | pMiniT2<br/> | style="width: 49px;" | <br/> | style="width: 345px;" | <br/> | style="width: 345px;" | gene fragment from IDT<br/> |- | style="width: 100px;" | 0 | style="width: 100px;" | HA, 1FRG<br/> | style="width: 26px;" | L | style="width: 47px;" | 3162<br/> | style="width: 171px;" | pCMV5-1FRG-HA-VL-kappa<br/> | style="width: 194px;" | <br/> | style="width: 194px;" | 3130 pCMV5-HA-VL-mIgKappa<br/> | style="width: 49px;" | 767 | style="width: 345px;" | BamH1-Not1 | style="width: 345px;" | subcloning from 3159 |- | style="width: 100px;" | <s>1</s> | style="width: 100px;" | <s>HA, 1FRG</s><br/> | style="width: 26px;" | <s>H</s> | style="width: 47px;" | <s>3158</s><br/> | style="width: 171px;" | <s>pCMV5-HA-VH-mIgG2a</s><br/> | style="width: 194px;" | <br/> | style="width: 194px;" | <s>3129 pCMV5-HA-VH-mIgG2b</s><br/> | style="width: 49px;" | <s>1471</s> | style="width: 345px;" | <s>BsrG1-Not1</s> | style="width: 345px;" | <s>subcloning from 3160</s> |- | style="width: 100px;" | 0 | style="width: 100px;" | HA, 1FRG<br/> | style="width: 26px;" | H | style="width: 47px;" | <span style="color: rgb(0, 0, 0); font-family: Arial, Verdana, sans-serif; font-size: 12px;">3163</span><br/> | style="width: 171px;" | pCEH0-1FRG-HA-VH-mIgG2a<br/> | style="width: 194px;" | 3160 pMiniT-2-1FRG-HA-VH-mIgG2a<br/> | style="width: 194px;" | 3017 pCEH0<br/> | style="width: 49px;" | 1464 | style="width: 345px;" | BsrG1-Not1<br/> | style="width: 345px;" | subcloning from 3160<br/> |- | style="width: 100px;" | 0 | style="width: 100px;" | HA, 1FRG<br/> | style="width: 26px;" | L | style="width: 47px;" | 3164 | style="width: 171px;" | pCEL0-1FRG-HA-VL-kappa<br/> | style="width: 194px;" | 3159 pMiniT2-1FRG-HA-VL-kappa<br/> | style="width: 194px;" | 3016 pCEL0<br/> | style="width: 49px;" | 701 | style="width: 345px;" | BssHII --NheI<br/> | style="width: 345px;" | subcloning from 3159<br/> |- | style="width: 100px;" | 0 | style="width: 100px;" | Flag<br/> | style="width: 26px;" | L | style="width: 47px;" | 3010 <br/> | style="width: 171px;" | 3010-5-miniT2 | style="width: 194px;" | <br/> | style="width: 194px;" | pMiniT2 | style="width: 49px;" | <br/> | style="width: 345px;" | <br/> | style="width: 345px;" | <span style="color:#FF0000;">sequenced, good</span> |- | style="width: 100px;" | 0 | style="width: 100px;" | <br/> | style="width: 26px;" | H | style="width: 47px;" | 3009<br/> | style="width: 171px;" | 3009-4-pMiniT2-Flag-H <br/> | style="width: 194px;" | <br/> | style="width: 194px;" | pMiniT2<br/> | style="width: 49px;" | <br/> | style="width: 345px;" | <br/> | style="width: 345px;" | 3009-4-pMiniT2, <span style="color:#FF0000;">good, sequenced</span><br/> |- | style="width: 100px;" | 0 | style="width: 100px;" | <br/> | style="width: 26px;" | L | style="width: 47px;" | <span style="color:#FF0000;">3010</span> | style="width: 171px;" | pCMV5-Flag-kappa<br/> | style="width: 194px;" | 3010-5-miniT2<br/> | style="width: 194px;" | <s>2493 pCMV5-Flag-kappa</s> 3130 pCMV5-HA-VL-mIgKappa | style="width: 49px;" | <br/> | style="width: 345px;" | BamH1+ Kpn1<br/> | style="width: 345px;" | <font color="#ff0000">need to do subcloning from </font>3010-5-miniT2 |- | style="width: 100px;" | -2 | style="width: 100px;" | <br/> | style="width: 26px;" | H | style="width: 47px;" | 3009 | style="width: 171px;" | pCMV5-Flag-VH-IgG2b<br/> | style="width: 194px;" | 3009-4-pMiniT2-Flag-H <br/> | style="width: 194px;" | <br/>3129 pCMV5-HA-VH-mIgG2b | style="width: 49px;" | 432 | style="width: 345px;" | BsrG1-Kpn1 | style="width: 345px;" | <span style="color: rgb(255, 0, 0);">subclone</span> from 3009-4-pMiniT2 using BsrG1-Kpn1 |- | style="width: 100px;" | 0 | style="width: 100px;" | <br/> | style="width: 26px;" | H | style="width: 47px;" | 3152<br/> | style="width: 171px;" | pCEH0-Flag-VH-mIgG2a<br/> | style="width: 194px;" | 3009-4-pMiniT2 <br/> | style="width: 194px;" | <span style="color:#FF0000;"><span style="font-family: Arial, Verdana, sans-serif; font-size: 12px;">3163 pCEH0-1FRG-HA-VH-mIgG2a</span></span><br/> | style="width: 49px;" | 371 | style="width: 345px;" | <s>BsrG1-Nhe1</s> BsrG1-Kpn1 | style="width: 345px;" | <span style="color:#FF0000;">subclone</span> from 3009-4-pMiniT2 using <s>BsrG1-Nhe1</s> VHSEVQLQQSGGELAKPGASVKMSCKSSGYTFTAYAIHWAKQAAGAGLEWIGYIAPAAGAAAYNAAFKGKATLAADKSSSTAYMAAAALTSEDSAVYYCARAAAAGADYWGQGTTLTVSSAS |} --[[User:Paul.xu|Paul.xu]] ([[User_talk:Paul.xu|talk]]) 07:20, 13 February 2018 (EST) [[Category:Antibodies]] [[Category:To Do]] [[Category:Tasks]]
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