Tag Antibodies
HA Tag antibodies
[edit]| Accession | Name | Clone |
Heavy | Light | Epitope |
| 1FRG |
12CA5 |
FAB 26/9 |
EVLLVESGGDLVKPGGFLKLSCAASGFTFSSFGMS |
DIVMTQSPSSLTVTAGEKVTMSCKSSQSLFNSG |
XDVPDYASLX |
| 1IFH |
FAB 17/9 |
EVQLVESGGDLVKPGGSLKLSCAASGFSFSSYGM SWVRQTPDKRLEWVATISNGGGYTYYPDSVKGR FTISRDNAKNTLYLQMSSLKSEDSAMYYCARRE RYDENGFAYWGQGTLVTVSAAKTTAPSVYPLAP VCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSL SSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNV AHPASSTKVDKKIEPR |
DIVMTQSPSSLTVTAGEKVTMSCTSSQSLFNSGKQK |
XDVPDYAS | |
Heavy
1FRG 1 EVLLVESGGDLVKPGGFLKLSCAASGFTFSSFGMSWVRHTPDKRLEWVATISNGGGYTYY 60
EV LVESGGDLVKPGG LKLSCAASGF+FSS+GMSWVR TPDKRLEWVATISNGGGYTYY
1IFH 1 EVQLVESGGDLVKPGGSLKLSCAASGFSFSSYGMSWVRQTPDKRLEWVATISNGGGYTYY 60
1FRG 61 QDSVKGRFTISRDNAKNTLFLEMTSLKSEDAGLYYCARRERYDEKGFAYWGRGTLVTVSA 120
DSVKGRFTISRDNAKNTL+L+M+SLKSED+ +YYCARRERYDE GFAYWG+GTLVTVSA
1IFH 61 PDSVKGRFTISRDNAKNTLYLQMSSLKSEDSAMYYCARRERYDENGFAYWGQGTLVTVSA 120
Light
1FRG 1 DIVMTQSPSSLTVTAGEKVTMSCKSSQSLFNSGKRKNFLTWYHQKPGQPPKLLIYWASTR 60
DIVMTQSPSSLTVTAGEKVTMSC SSQSLFNSGK+KN+LTWY QKPGQPPK+LIYWASTR
1IFH 1 DIVMTQSPSSLTVTAGEKVTMSCTSSQSLFNSGKQKNYLTWYQQKPGQPPKVLIYWASTR 60
1FRG 61 ESGVPDRFSGSGSGTDFTLTITSVQAEDLAIYYCQNDYSHPLTFGAGTKLELKRADAAPT 120
ESGVPDRF+GSGSGTDFTLTI+SVQAEDLA+YYCQNDYS+PLTFG GTKLELKRADAAPT
1IFH 61 ESGVPDRFTGSGSGTDFTLTISSVQAEDLAVYYCQNDYSNPLTFGGGTKLELKRADAAPT 120
| |||||
Tag Antibodies
[edit]| Urgency | Name | Chain | Clone ID | Name | Parent | Vector | Insert Size | Enzymes | Status/note |
| 0 | Vector |
L | 3154 |
pCMV5-HA-VH-mKappa |
HA 3129 pMiniT2-3129-1 |
2493 pCMV5-mIgG-kLC |
527 | BamH1-Kpn1 |
sequenced, good, glycerol stock made |
| - |
insert in pMiniT2: BssHII - Xba1 |
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| - |
| ||||||||
| 0 | His | H+L, sc | 3002 |
pJet1.2-Anti-His Tag 3D5 |
pJet1.2 |
Nde1-Sal1/Xho1 | 3129-Kpn1-F1, sequenced, good, need to subclone into pET30b, but there is no tag for purification | ||
| 0 | His | L | 3001 |
pMiniT2-His-VL |
good, sequenced | ||||
| 0 | H |
3135 |
3135-3-pMiniT2 |
pMiniT2 |
in pMiniT2 vector now, 3135-3-pMiniT2 sequenced, good, need to subclone into 3129 pCMV5-HA-VH-mIgG2b using BsrG1-Kpn1 | ||||
| 0 | L | 3001 |
pCMV5-His-VL-kappa |
3001-pMiniT2 |
3130 pCMV5-HA-VL-mIgKappa |
435 | BamH1-Kpn1 |
3001-pMiniT2 is good, sequenced subclone from 3001-pMiniT2, | |
| -2 | H | 3135 | pCMV5-His-VH-IgG2b |
3135-3-pMiniT2 |
3129 pCMV5-HA-VH-mIgG2b
|
364 | BsrG1-Kpn1 |
subclone into 3129 pCMV5-HA-VH-mIgG2b using BsrG1-Kpn1 from 3135-3-pMiniT2 | |
| 0 | H | 3168 |
pCEH0-His-VH-mIgG2a |
3135-3-pMiniT2 |
3163 pCEH0-1FRG-HA-VH-mIgG2a |
358 | BsrG1-Nhe1 |
3135-3-pMiniT2 VHSQVQLQQSGPEDVKPGASVKISCKASGYTFTDYYMNWVKQSPGKGLEWIGDINPNNGGTSYNQKFKGRATLTVDKSSSTAYMELRSLTSEDSSVYYCESQSGAYWGQGTTVTVSAASS | |
| 0 | Myc |
L | 3003 |
3003-L-5 |
pJet1.2 |
IL2 signal peptide, BamH1-Kpn1, good | |||
| 0 | H | 3003 | 3003-H-1 |
pJet1.2 |
need to use 3076-myc-BsrG1-F to fix frame shift | ||||
| 0 | L | 3075 |
pCMV5-Myc-L-kappa |
2493 pCMV5-mIgG-kLC |
completed, subclone from 3003-L5 using BamH1-Kpn1 to 2493 pCMV5-mIgG-kLC sequenced, good | ||||
| -2 | H | 3076 |
pCMV5-Myc-H-IgG2b |
3129 pCMV5-HA-VH-mIgG2b |
BsrG1-Kpn1 |
PCR from 3003-H-1 in pJet1.2, using 3076-myc-BsrG1-F and 3076-myc-Nhe1-Kpn1-R in pMiniT2 now sequenced, good
| |||
| 0 | H | 3167 |
pCEH0-Myc-VH-mIgG2a |
3027-pMiniT2 (3076 myc pMiniT2) |
3163 pCEH0-1FRG-HA-VH-mIgG2a |
397 | BsrG1-Nhe1 |
subcloning from the misslabeled myc clone in pMiniT2 EVDLVESGGDLVKPGGSLKLSCAASGFTFSHYGMSWVRQTPDKRLEWVATIGSRGTYTHYPDSVKGRFTISRDNDKNALYLQMNSLKSEDTAMYYCARRSEFYYYGNTYYYSAMDYWGQGTTVTVSS | |
| 0 | HA, 1IFH |
sc VH+VL | 3074 |
pT7A30-HA-Avi-BirA |
pJet1.2 | 3074-1-PJET1-2 is good, need to subclone into 1834 pT7A30-Avi-BirA | |||
| 0 | HA, 1IFH |
L | 3011 |
pJet1.2-HA-L-1 |
pJet1.2 |
3011-L-1 is good, sequenced | |||
| 0 | HA, 1IFH |
H | 3011 |
pJet1.2-HA-H-2 |
pJet1.2 |
3011-H-2 is good, sequenced | |||
| 0 | HA, 1IFH |
L | 3030 | 3130-4-pMiniT2 |
pMiniT2 |
3130-4-pMiniT2 is good (sequenced), derived from 3011-L-1 pJet1.2-HA light chain by PCR using 3130-BamH1-R + 3130-Kpn1-R | |||
| 0 | HA, 1IFH |
L | 3130 |
pCMV5-HA-VL-mIgKappa |
3130-4-pMiniT2 |
2493 pCMV5-mIgG-kLC |
429bp |
subclone from using BamH1+ Kpn1 |
completed,good, sequenced, need to make glycerol stock |
| 0 | HA, 1IFH |
H | 3129 | pMiniT2-3129-1 | 3011 pJet1.2-HA Heavy chain |
pMiniT2 |
PCR | good sequenced | |
| 0 | HA, 1IFH |
H | 3129 |
pCMV5-HA-VH-mIgG2b |
2492 pCMV5-mIgG2b-HC | 3154 pCMV5-HA-VH-mKappa |
subcloning Kpn1 -Not1 | subcloning mIgG2b constant domain to 3154, due to BamH1 site | |
| 0 | HA, 1IFH |
L | 3131 |
pCEL0-HA-VL-mIgKappa |
3011 pJet1.2-HA-L-1 |
3021 pCEL0-mIg-LCKappa |
342 |
BssHII-Xho1 |
subclone 342 bp fragment from 3011 pJet1.2-HA-L-1 using BssHII-Xho1 do not use the smallest fragment |
| 0 | HA, 1IFH |
H | 3132 |
pCEH0-HA-VH-mIgG2a |
3011 pJet1.2-HA-H-2 |
3163 pCEH0-1FRG-HA-VH-mIgG2a |
373 |
BsrG1-Nhe1 |
subclone 373 bp fragment from 3011 pJet1.2-HA-H-2 using BsrG1-Nhe1 |
| 0 | HA, 1FRG |
L | 3159 |
pMiniT2-1FRG-HA-VL-kappa |
pMiniT2 | gene fragment from IDT | |||
| 0 | HA, 1FRG |
H | 3160 |
pMiniT-2-1FRG-HA-VH-mIgG2a |
pMiniT2 |
gene fragment from IDT | |||
| 0 | HA, 1FRG |
L | 3162 |
pCMV5-1FRG-HA-VL-kappa |
3130 pCMV5-HA-VL-mIgKappa |
767 | BamH1-Not1 | subcloning from 3159 | |
| 0 | HA, 1FRG |
H | 3163 |
pCEH0-1FRG-HA-VH-mIgG2a |
3160 pMiniT-2-1FRG-HA-VH-mIgG2a |
3017 pCEH0 |
1464 | BsrG1-Not1 |
subcloning from 3160 |
| 0 | HA, 1FRG |
L | 3164 | pCEL0-1FRG-HA-VL-kappa |
3159 pMiniT2-1FRG-HA-VL-kappa |
3016 pCEL0 |
701 | BssHII --NheI |
subcloning from 3159 |
| 0 | Flag |
L | 3010 |
3010-5-miniT2 | pMiniT2 | sequenced, good | |||
| 0 | H | 3009 |
3009-4-pMiniT2-Flag-H |
pMiniT2 |
3009-4-pMiniT2, good, sequenced | ||||
| 0 | L | 3010 | pCMV5-Flag-kappa |
3010-5-miniT2 |
3130 pCMV5-HA-VL-mIgKappa |
BamH1+ Kpn1 |
need to do subcloning from 3010-5-miniT2 | ||
| -2 | H | 3009 | pCMV5-Flag-VH-IgG2b |
3009-4-pMiniT2-Flag-H |
|
432 | BsrG1-Kpn1 |
subclone from 3009-4-pMiniT2 using BsrG1-Kpn1 | |
| 0 | H | 3152 |
pCEH0-Flag-VH-mIgG2a |
3009-4-pMiniT2 |
3163 pCEH0-1FRG-HA-VH-mIgG2a |
371 |
BsrG1-Kpn1 |
subclone from 3009-4-pMiniT2 using VHSEVQLQQSGGELAKPGASVKMSCKSSGYTFTAYAIHWAKQAAGAGLEWIGYIAPAAGAAAYNAAFKGKATLAADKSSSTAYMAAAALTSEDSAVYYCARAAAAGADYWGQGTTLTVSSAS |