Editing 1662-pHot (section)

== '''Results'''  ==

=== cloning  ===

digest vector1658 pCP40 with EcoR1 + Xba1,Ligate to annealed oligos 

EcoR1-MCS-Xba1-T + EcoR1-MCS-Xba1-B<br> 

<br> 

new MCS Nco1 Nhe1 BamH1 EcoR1 HindIII Not1 Xho1 Xba1<br>AATTTAAGAAGGAGATATACC ATG GGC GCT AGC GGA TCC GAA TTC AAGCTT GCGGCCGCACTCGAG T<br> aTTCTTCCTCTATATGG TAC CCG CGA TCG CCT AGG CTT AAG TTCGAA CGCCGGCGTGAGCTC AGATC 

[[Image:Digest for 1773 1775 1662.JPG]] 

<br> 

=== Colony Screening  ===

Date: 2013-3-7 

Make miniprep and digest with Nde1 + Xho1<br> 

[[Image:1658DIGEST WITH NdeI+XhoI.JPG]][[Image:1662digest.JPG]]<br> 

sent 1662-1 to sequence,no priming<br>