1662-pHot
Cloning of 1662-pHot
[edit]Purpose: Cloning of 1662-pHot
Date: 2013-3-5---2013-3-7
Notebook:0
Page:
Clone ID:1662
Experimenters: yingying
Results
[edit]cloning
[edit]
Colony Screening
[edit]Date: 2013-3-7
Make miniprep and digest with Nde1 + Xho1
Comment by Paul: This clone is Junk!
Can not be sequened, and the gel picture shows this is a junk clone
Result intepretation/Summary
[edit]
Conclusion
[edit]Cloning of 1662-pHot
[edit]Purpose: Cloning of 1662-pHot
Date: 2013-7
Notebook:0
Page:
Clone ID:1662
Experimenters: shanyi
Results
[edit]cloning
[edit]digest vector1658 pCP40 with EcoR1 + Xba1,Ligate to annealed oligos
EcoR1-MCS-Xba1-T + EcoR1-MCS-Xba1-B
new MCS Nco1 Nhe1 BamH1 EcoR1 HindIII Not1 Xho1 Xba1
AATTTAAGAAGGAGATATACC ATG GGC GCT AGC GGA TCC GAA TTC AAGCTT GCGGCCGCACTCGAG T
aTTCTTCCTCTATATGG TAC CCG CGA TCG CCT AGG CTT AAG TTCGAA CGCCGGCGTGAGCTC AGATC
Colony Screening
[edit]Date: 2013-3-7
Make miniprep and digest with Nde1 + Xho1
sent 1662-1 to sequence,no priming
Redo Cloning of 1662-pHot
[edit]Purpose: Cloning of 1662-pHot
Date: 2013-7
Notebook:0
Page:
Clone ID:1662
Experimenters: shanyi
Results
[edit]cloning
[edit]digest vector1658 pCP40 with EcoR1 + Xba1,Ligate to annealed oligos
EcoR1-MCS-Xba1-T + EcoR1-MCS-Xba1-B
new MCS Nco1 Nhe1 BamH1 EcoR1 HindIII Not1 Xho1 Xba1
AATTTAAGAAGGAGATATACC ATG GGC GCT AGC GGA TCC GAA TTC AAGCTT GCGGCCGCACTCGAG T
aTTCTTCCTCTATATGG TAC CCG CGA TCG CCT AGG CTT AAG TTCGAA CGCCGGCGTGAGCTC AGATC
Colony Screening
[edit]Date: 2013-7-17
Make miniprep and digest with Nde1 + Xho1
Result intepretation/Summary
[edit]