Editing 10 X PBS

== Protocol 1<br>  ==

22.8 g NaH<sub>2</sub>PO<sub>4</sub> (mw=120; 0.038M)&nbsp; or 26.2g NaH<sub>2</sub>PO<sub>4</sub>(H2O) (mw=137.99; 0.038M) (Note: monobasic)<br>115g Na<sub>2</sub>HPO<sub>4</sub> (mw=141.96; 0.162M) (Note: dibasic)<br>438.4g NaCl 

Make it up 

Add NaH<sub>2</sub>PO<sub>4</sub> and Na<sub>2</sub>HPO<sub>4</sub> and NaCl to 4500 ml dH2O.<br> 

<br>Adjust pH to 7.4 by adding dilute (1N) HCl if necessary.<br> 

<br>Bring the final volume to 5000 ml with water.<br> 

<br>Dilute 1:10 for final concentration.<br> 

== Protocol 2  ==

137 mM NaCl<br> 2.7 mM KCl<br> 100 mM Na<sub>2</sub>HPO<sub>4</sub><br> 2 mM KH<sub>2</sub>PO<sub>4</sub> 

To make 10X PBS Buffer, dissolve the following reagents in 800 ml ultrapure water.<br>NaCl 80 g<br>KCl&nbsp;&nbsp; 2 g<br>Na2HPO4 14.4 g<br>KH2PO4 2.4 g<br> 

Adjust pH of PBS Buffer Solution to 7.4 with HCl.<br>Bring volume to 1 liter, autoclave or sterilize by filtration.<br><br> 

== Protocol 3  ==

A 10 liter stock of 10x PBS can be prepared by dissolving 800 g NaCl, 20 g KCl, 144 g<br>Na2HPO4 · 2H2O and 24 g KH2PO4 in 8 L of distilled water, and topping up to 10 L. The<br>pH is ~6.8, but when diluted to 1x PBS it should change to 7.4. When making buffer<br>solutions, it is good practice to always measure the pH directly using a pH meter. If<br>necessary, pH can be adjusted using hydrochloric acid or sodium hydroxide.<br>On dilution, the resultant 1x PBS should have a final concentration of 137 mM NaCl, 10<br>mM Phosphate, 2.7 mM KCl, and a pH of 7.4.<br> 

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== PBS 20x stock  ==

<br>Potassium chloride 4 g<br>NaCl 160 g<br>Potassium phosphate monobasic 4 g<br>Sodium phosphate dibasic (7•H2O) DI 43.2 g<br><br><br> 

[[Category:Buffer_Recipe]]