10 X PBS

Protocol 1

22.8 g NaH₂PO₄ (mw=120; 0.038M)  or 26.2g NaH₂PO₄(H2O) (mw=137.99; 0.038M) (Note: monobasic)
115g Na₂HPO₄ (mw=141.96; 0.162M) (Note: dibasic)
438.4g NaCl

Make it up

Add NaH₂PO₄ and Na₂HPO₄ and NaCl to 4500 ml dH2O.

Adjust pH to 7.4 by adding dilute (1N) HCl if necessary.

Bring the final volume to 5000 ml with water.

Dilute 1:10 for final concentration.

Protocol 2

137 mM NaCl
2.7 mM KCl
100 mM Na₂HPO₄
2 mM KH₂PO₄

To make 10X PBS Buffer, dissolve the following reagents in 800 ml ultrapure water.
NaCl 80 g
KCl   2 g
Na2HPO4 14.4 g
KH2PO4 2.4 g

Adjust pH of PBS Buffer Solution to 7.4 with HCl.
Bring volume to 1 liter, autoclave or sterilize by filtration.

Protocol 3

A 10 liter stock of 10x PBS can be prepared by dissolving 800 g NaCl, 20 g KCl, 144 g
Na2HPO4 · 2H2O and 24 g KH2PO4 in 8 L of distilled water, and topping up to 10 L. The
pH is ~6.8, but when diluted to 1x PBS it should change to 7.4. When making buffer
solutions, it is good practice to always measure the pH directly using a pH meter. If
necessary, pH can be adjusted using hydrochloric acid or sodium hydroxide.
On dilution, the resultant 1x PBS should have a final concentration of 137 mM NaCl, 10
mM Phosphate, 2.7 mM KCl, and a pH of 7.4.

PBS 20x stock

Potassium chloride 4 g
NaCl 160 g
Potassium phosphate monobasic 4 g
Sodium phosphate dibasic (7•H2O) DI 43.2 g