Editing 1636 1545 1544 1543 1992 1680 1694 2092

== Protein Expression  ==

2014/6/4<br> SHANYI use 20ul magnetic beads 

[[Image:1636 1545 1544 1543 1992 1680 1694 2092.jpg]]<br> 

{| width="832" cellspacing="1" cellpadding="1" border="1"
|-
| Lane<br> 
| Name<br> 
| Protein Name<br> 
| Clone ID<br> 
| Host<br> 
| Induction<br> 
| SAMPLE<br> 
| pI/Mw<br> 
| express<br>
|-
| 1<br> 
| rowspan="1" | pT7A3-VVCap-S-GGGS(with 3X GGGS linker)<br> 
| rowspan="1" | VVCap-S-GGGS<br> 
| rowspan="1" | 
1636<br> 

| rowspan="1" | 
15 ml, 

Nico21(DE3)plysN<br> 

| rowspan="1" | 0.3mM IPTG RT O/N<br> 
| s<br> 
| rowspan="1" | 
8.67/38<br> 

| NO, cell died<br>
|-
| 2<br> 
| rowspan="1" | pT7A3-VVCap<br> 
| rowspan="1" | <br> 
| rowspan="1" | 1545<br> 
| rowspan="1" | 15 ml, 
Nico21(DE3)plysN<br> 

| rowspan="1" | 0.3mM IPTG RT O/N<br><br> 
| s<br> 
| rowspan="1" | 8.85/72<br> 
| no<br>
|-
| 3<br> 
| rowspan="1" | <br> 
| rowspan="1" | <br> <br> 
| rowspan="1" | <br> 1545<br> 
| rowspan="1" | 15 ml, 
Nico21(DE3)plysN<br> 

| rowspan="1" | 0.3mM IPTG RT O/N<br><br> 
| p<br> 
| rowspan="1" | <br> 
| <br>
|-
| 4<br> 
| rowspan="1" | pT7A3-VVCap-L(large subunit)<br> 
| rowspan="1" | <br> 
| rowspan="1" | 1544<br> 
| rowspan="1" | 15 ml, 
Nico21(DE3)plysN<br> 

| rowspan="1" | 0.3mM IPTG RT O/N<br><br> 
| s<br> 
| rowspan="1" | 8.74/36.9<br> 
| no<br>
|-
| 5<br> 
| rowspan="1" | pT7A3-VVCap-S(small subunit )<br> 
| rowspan="1" | <br> 
| rowspan="1" | 1543<br> <br> 
| rowspan="1" | 15 ml, 
Nico21(DE3)plysN<br> 

| rowspan="1" | 0.3mM IPTG RT O/N<br><br> 
| s<br> 
| rowspan="1" | 8.82/33.4<br> 
| no<br>
|-
| 6<br> 
| rowspan="1" | pL42-SM2<br> 
| rowspan="1" | Monomer Benzonase H184R<br> 
| rowspan="1" | 
<br> 1992<br> 

| rowspan="1" | 15 ml, 
Nico21(DE3)plysN<br> 

| rowspan="1" | 0.3mM IPTG RT O/N<br><br> 
| s<br> 
| rowspan="1" | 6.54/27.9<br> 
| no <br>
|-
| 7<br> 
| 
pageRuler Plus Prestained protein ladder<br> 

| <br> 
| <br> 
| <br> 
| <br> 
| <br> 
| 
<br> 

| <br>
|-
| 8<br> 
| rowspan="1" | pCold-GST-wtSM2<br> 
| rowspan="1" | <br> 
| rowspan="1" | <br> 1680<br> 
| rowspan="1" | 15 ml, 
Nico21(DE3)plysN<br> 

| rowspan="1" | 0.3mM IPTG RT O/N<br> 
| s<br> 
| 
6.18/60.3<br> 

| <br>
|-
| 9<br> 
| rowspan="1" | pT7A3-wtSM2<br> 
| rowspan="1" | &nbsp;&nbsp; <br> 
| rowspan="1" | 1694<br> 
| rowspan="1" | 
&nbsp;15 ml, 

Nico21(DE3)plysN <br> 

| rowspan="1" | 0.3mM IPTG RT O/N<br><br> 
| s<br> 
| 
<br> 

| <br>
|-
| 10<br> 
| <br> <br> 
| <br> 
| 1694<br> 
| rowspan="1" | 15 ml, 
Nico21(DE3)plysN<br> 

| rowspan="1" | 0.3mM IPTG RT O/N<br> 
| p<br> 
| rowspan="1" | <br> 
| <br>
|-
| 11<br> 
| pT7A3-MBP-i3C-6H<br> 
| <br> 
| 2092<br> 
| rowspan="1" | 15 ml, 
Nico21(DE3)plysN<br> 

| rowspan="2" | 0.3mM IPTG RT O/N<br> 
| S<br> 
| rowspan="1" | <br> 
| <br>
|-
| 12<br> 
| <br> 
| <br> 
| 2092<br> 
| rowspan="1" | 15 ml, 
Nico21(DE3)plysN<br> 

| p<br> 
| <br> 
| <br>
|}

need to redo 

pL42 vector: induce at 42 oC for 2~3 hours, grow at 30 oc<br> 

1680, 1694 with C-CBD, NO his tag<br> 

<br> 

<br> 

[[Category:Protein_Purification]]