1636 1545 1544 1543 1992 1680 1694 2092
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Protein Expression
[edit]2014/6/4
SHANYI use 20ul magnetic beads
| Lane |
Name |
Protein Name |
Clone ID |
Host |
Induction |
SAMPLE |
pI/Mw |
express |
| 1 |
pT7A3-VVCap-S-GGGS(with 3X GGGS linker) |
VVCap-S-GGGS |
1636 |
15 ml, Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
8.67/38 |
NO, cell died |
| 2 |
pT7A3-VVCap |
1545 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
8.85/72 |
no | |
| 3 |
1545 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
p |
||||
| 4 |
pT7A3-VVCap-L(large subunit) |
1544 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
8.74/36.9 |
no | |
| 5 |
pT7A3-VVCap-S(small subunit ) |
1543 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
8.82/33.4 |
no | |
| 6 |
pL42-SM2 |
Monomer Benzonase H184R |
|
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
6.54/27.9 |
no |
| 7 |
pageRuler Plus Prestained protein ladder |
|
||||||
| 8 |
pCold-GST-wtSM2 |
1680 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
6.18/60.3 |
||
| 9 |
pT7A3-wtSM2 |
|
1694 |
15 ml, Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
|
|
| 10 |
1694 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
p |
||||
| 11 |
pT7A3-MBP-i3C-6H |
2092 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
S |
|||
| 12 |
2092 |
15 ml,
Nico21(DE3)plysN |
p |
need to redo
pL42 vector: induce at 42 oC for 2~3 hours, grow at 30 oc
1680, 1694 with C-CBD, NO his tag