Editing
1737 2038Taq 2288 1736 cre 2289 1898 1617 211
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
Aug4,2014<br> [[Image:1737 2038 2288.jpg]]<br> {| width="485" cellspacing="1" cellpadding="1" border="1" |- | Lane<br> | Clone ID<br> | Desc<br> | Induction<br> | Purification<br> | MW, KDa<br> | purification<br> |- | 1<br> | rowspan="3" | 1737 Lot number: 65-1737<br> | rowspan="3" | <br> pT7A3E-His-TRX<br> <br> His-thioredoxin for making T7 DNA Polymerase complex | rowspan="3" | ?mMIPTG, 25 oC o/n <br> <br> | rowspan="3" | Ni <br> <br> | rowspan="3" | 5.72/13.4 <br> | rowspan="3" | fraction2,3,6<br> |- | 2<br> |- | 3<br> |- | 4<br> | pageRulerPlusPrestained protein ladder<br> | <br> | <br> | <br> | <br> | <br> |- | 5<br> | rowspan="5" | 2038 <br> | rowspan="5" | <br> <br> <br> L42-sso7d-KlenTaq<br> <br> | rowspan="5" | <br> | rowspan="5" | Ni <br> <br> | rowspan="5" | 7.02/70.1<br> | rowspan="5" | fraction4,5,6,7,8<br> |- | 6<br> |- | 7<br> |- | 8<br> |- | 9<br> |- | 10<br> | rowspan="3" | 2288 Lot number: 63-2288<br> | rowspan="3" | pT7A3E-6H-HC-SMT3 <br> SUMO tag Saccharomyces cerevisiae S288c SUMO family protein SMT3 (SMT3)<br> | rowspan="3" | ?mMIPTG, 25 oC o/n <br> <br> | rowspan="3" | Ni <br> <br> <br> | rowspan="3" | <br> 5.81/13.6<br> <br> | rowspan="3" | fraction6,7,10<br> |- | 11<br> |- | 12<br> |} <br> [[Image:1736 cre.jpg]]<br> <br> {| width="485" cellspacing="1" cellpadding="1" border="1" |- | Lane<br> | Clone ID<br> | Desc<br> | Induction<br> | Purification<br> | MW, KDa<br> | purification<br> |- | 1<br> | rowspan="4" | 1736<br> Lot number: 64-1736<br> | rowspan="4" | pT7A3-His-NusA <br> <br> | rowspan="4" | ?mMIPTG, 25 oC o/n <br> <br> | rowspan="4" | Ni <br> <br> | rowspan="4" | 4.70/61577.24 <br> <br> | rowspan="4" | NusA1<br> fraction7,11,14,20<br> |- | 2<br> |- | 3<br> |- | 4<br> |- | 5<br> | pageRulerPlusPrestained protein ladder<br> | <br> | <br> | <br> | <br> | <br> |- | 6<br> | rowspan="3" | 1736 Lot number: 64-1736<br> | rowspan="3" | <br> <br> pT7A3-His-NusA <br> | rowspan="3" | ?mMIPTG, 25 oC o/n<br> | rowspan="3" | Ni <br> <br> | rowspan="3" | 4.70/61577.24<br><br> | rowspan="3" | NusA2 fraction12,14,19<br> |- | 7<br> |- | 8<br> |- | 9<br> | rowspan="4" | <br> | rowspan="4" | <br> CRE<br> | rowspan="4" | ?mMIPTG, 25 oC o/n<br> <br> | rowspan="4" | Ni <br> <br> <br> | rowspan="4" | 43<br> | rowspan="4" | fraction8,12,13,15<br> |- | 10<br> |- | 11<br> |- | 12<br> |} <br> [[Image:Cre 2289 1737 bst phi29 taq-muts.jpg]]<br> <br> {| width="485" cellspacing="1" cellpadding="1" border="1" |- | Lane<br> | Clone ID<br> | Desc<br> | Induction<br> | Purification<br> | MW, KDa<br> | Expression<br> |- | 1<br> | <br> | cre<br> | ?mMIPTG, 25 oC o/n<br> | Ni<br> | 43<br> | fraction17<br> |- | 2<br> | rowspan="4" | 2289<br> | rowspan="4" | <br> pT7A30-SMT3<br> SUMO tag Saccharomyces cerevisiae S288c SUMO family protein SMT3 (SMT3), C-His tag<br> <br> <br> | rowspan="4" | ?mMIPTG, 25 oC o/n<br> | rowspan="4" | Ni <br> <br> | rowspan="4" | 5.42 / 14.1<br> | rowspan="4" | fraction7,8,9,10<br> |- | 3<br> |- | 4<br> |- | 5<br> |- | 6<br> | pageRulerPlusPrestained protein ladder<br> | <br> | <br> | <br> | <br> | <br> |- | 7<br> | rowspan="3" | 1737 Lot number: 65-1737<br> | rowspan="3" | pT7A3E-His-TRX <br>His-thioredoxin for making T7 DNA Polymerase complex<br> | rowspan="3" | ?mMIPTG, 25 oC o/n<br> | rowspan="3" | Ni <br> | rowspan="3" | 5.72/13.4<br> <br> | rowspan="3" | fraction5,6,13 <br> |- | 8<br> |- | 9<br> |- | 10<br> | 1898 Lot number: 621898<br> | pT7A3-8H-Bst-Exo- hermostable Bacillus DNA polymerase I large fragment, Bst<br> | 0.1mM IPTG RT induce o/n,<br> | RocheNicolumn Giga Q-650M<br> | 5.86 / 69<br> | 10.5mg/ml<br> |- | 11<br> | 1512 Lot number: 661512<br> | pT7A3-Phi29DNAPol Phi29 DNA Polymerase, wild type<br> | 0.2mM IPTG RT induce o/n,<br> | RocheNicolumn Giga sp-650M<br> | 8.30 / 67.8<br><br> | 1.47mg/ml<br> |- | 12<br> | 211 Lot number: 61211<br> | pDBHisMBP-TaqMuts<br> | 0.1mM IPTG 37oc induce o/n,<br> | RocheNicolumn Giga Q-650M<br> | 5.40 / 134102.41 <br><br> | 14mg/ml<br> |} <br> <br> [[Category:Protein_Purification]][[Category:QC]]
Summary:
Please note that all contributions to ELN may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ELN:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Category:
Main page
To Do
Tasks
My ELN
Weekly Reports
Buffer Recipe
SOP
Protocols
Inventories
Projects
Products
Customers
Contacts
Suppliers
Vectors
Vector Map
Clones
Cloning
Sequencing
NGS
Protein Expression
Purchaseing
QC
293 Expression
CHO Expression
Sf9 Expression
Protein Purification
Stem Cells
Cytokines
Protein Kinases
GPCR
Cell Lines
Competent Cells
Viral Proteins
VLP
Baculovirus
AAV
Adenovirus
Lentivirus
Virus Titering
Antibodies
Transfection
ELISA
qPCR RT-PCR
ImmunoAssay
Multiplex BioAssa
Lateral_Flow
Conjugation
Compounds Synthesis
BioEngineering
GenomeEngineering
Services
Software
Ideas
Links
Knowledgebase
Employees
Company
Tools
What links here
Related changes
Page information
Navigation
Special pages