1737 2038Taq 2288 1736 cre 2289 1898 1617 211

Aug4,2014

Lane	Clone ID	Desc	Induction	Purification	MW, KDa	purification
1	1737 Lot number: 65-1737	pT7A3E-His-TRX His-thioredoxin for making T7 DNA Polymerase complex	?mMIPTG, 25 oC o/n	Ni	5.72/13.4	fraction2,3,6
2
3
4	pageRulerPlusPrestained protein ladder
5	2038	L42-sso7d-KlenTaq		Ni	7.02/70.1	fraction4,5,6,7,8
6
7
8
9
10	2288 Lot number: 63-2288	pT7A3E-6H-HC-SMT3 SUMO tag Saccharomyces cerevisiae S288c SUMO family protein SMT3 (SMT3)	?mMIPTG, 25 oC o/n	Ni	5.81/13.6	fraction6,7,10
11
12

Lane	Clone ID	Desc	Induction	Purification	MW, KDa	purification
1	1736   Lot number: 64-1736	pT7A3-His-NusA	?mMIPTG, 25 oC o/n	Ni	4.70/61577.24	NusA1 fraction7,11,14,20
2
3
4
5	pageRulerPlusPrestained protein ladder
6	1736 Lot number: 64-1736	pT7A3-His-NusA	?mMIPTG, 25 oC o/n	Ni	4.70/61577.24	NusA2 fraction12,14,19
7
8
9		CRE	?mMIPTG, 25 oC o/n	Ni	43	fraction8,12,13,15
10
11
12

Lane	Clone ID	Desc	Induction	Purification	MW, KDa	Expression
1		cre	?mMIPTG, 25 oC o/n	Ni	43	fraction17
2	2289	pT7A30-SMT3 SUMO tag Saccharomyces cerevisiae S288c SUMO family protein SMT3 (SMT3), C-His tag	?mMIPTG, 25 oC o/n	Ni	5.42 / 14.1	fraction7,8,9,10
3
4
5
6	pageRulerPlusPrestained protein ladder
7	1737 Lot number: 65-1737	pT7A3E-His-TRX His-thioredoxin for making T7 DNA Polymerase complex	?mMIPTG, 25 oC o/n	Ni	5.72/13.4	fraction5,6,13
8
9
10	1898 Lot number: 621898	pT7A3-8H-Bst-Exo- hermostable Bacillus DNA polymerase I large fragment, Bst	0.1mM IPTG RT induce o/n,	RocheNicolumn Giga Q-650M	5.86 / 69	10.5mg/ml
11	1512 Lot number: 661512	pT7A3-Phi29DNAPol Phi29 DNA Polymerase, wild type	0.2mM IPTG RT induce o/n,	RocheNicolumn Giga sp-650M	8.30 / 67.8	1.47mg/ml
12	211 Lot number: 61211	pDBHisMBP-TaqMuts	0.1mM IPTG 37oc induce o/n,	RocheNicolumn Giga Q-650M	5.40 / 134102.41	14mg/ml