Editing 2012-pL42-Dnase1

== '''Cloning of 2012-pL42-Dnase1'''  ==

'''Purpose:''' Cloning of 2012-pL42-Dnase1 

'''Date: '''2014-1-13---2014-1-16 

'''Notebook:0''' 

'''Page:''' 

'''Clone ID:2012''' 

'''Experimenters:''' yingying 

== '''Results'''  ==

=== cloning  ===

[[Image:718 1972 1973 1974 1997 2012pcr 1-2-2014.JPG]]<br> 

PCR DNase 1 from 202 using <br>DNAse1-Nde1-F <br>DNAse1-22<br> 

[[Image:1997 2012 2013 2024clone 1-14-2014.jpg]] 

{| width="200" cellspacing="1" cellpadding="1" border="1"
|-
| lane<br> 
| 3<br> 
| 4<br>
|-
| <br> 
| pcr<br> 
| 1984 pL42<br>
|-
| <br> 
| colspan="2" | Nde1 +Xho1<br>
|}

<br> 

=== Colony Screening  ===

Date: 2014-1-17 

Make miniprep and digest with Nde1 + Xho1 

[[Image:1997 2012digest 1-16-2014.jpg]]<br> 

<br> 

== '''Result intepretation/Summary'''  ==

== '''Conclusion'''  ==

== '''Cloning of 2012-pL42-Dnase1'''  ==

'''Purpose:''' Cloning of2012-pL42-Dnase1'''==''' 

'''Date: '''2014-7---- 

'''Notebook:0''' 

'''Page:''' 

'''Clone ID:2012''' 

'''Experimenters:'''&nbsp; shanyi 

== '''Results'''  ==

=== CLONING  ===

PCR DNase 1 from 202 using <br>DNAse1-Nde1-F atcgac cat atg ctg aagatcgcagctttcaacatc<br>DNAse1-22 (5/3/2011)<br>(CACTAAGGTACCCTCGAGAATCC)<br>GGATTCTCGAGGGTACCTTAGTG 

<br> 

<br> 

<br> clone into 1984 pL42 Nde1 +Xho1<br> 

[[Image:Digest for 2249 2012 2049 2197.JPG]]<br> 

<br> 

=== Colony Screening  ===

Date: 2014_7 

Make miniprep, use Nde1 +Xho1 to find positive clone<br> 

[[Image:1577 1578 2012 2049digest1.JPG]]<br> 

use PstI to verify<br> 

[[Image:2238 1577 1578 2186 2272 2012digest.JPG]] 

sent 2012-2 to sequence, missing one g<br> 

<br> 

== '''Cloning of 2012-pL42-Dnase1'''  ==

'''Purpose:''' Cloning of2012-pL42-Dnase1'''==''' 

'''Date: '''2014-8---- 

'''Notebook:0''' 

'''Page:''' 

'''Clone ID:2012''' 

'''Experimenters:'''&nbsp; shanyi 

== '''Results'''  ==

=== CLONING  ===

PCR DNase 1 from 2012-2 using <br>DNAse1-Nde1-F atcgac cat atg ctg aagatcgcagctttcaacatc<br>DNAse1-22 (5/3/2011)<br>(CACTAAGGTACCCTCGAGAATCC)<br>GGATTCTCGAGGGTACCTTAGTG 

[[Image:2297_2012fixpcr.JPG]]<br> clone into 1984 pL42 Nde1 +Xho1<br> 

[[Image:Digest_for2012fix_2166.JPG]]<br> 

<br> 

=== Colony Screening  ===

Date: 2014_7 

Make miniprep, use Nde1 +Xho1 to find positive clone<br> 

[[Image:2012FIX_2303_1578_2166digest.JPG]]

== '''Result intepretation/Summary'''  ==

*<br>

== '''Conclusion'''  ==

<br><br> 

[[Category:Cloning]]