2012-pL42-Dnase1
Cloning of 2012-pL42-Dnase1
[edit]Purpose: Cloning of 2012-pL42-Dnase1
Date: 2014-1-13---2014-1-16
Notebook:0
Page:
Clone ID:2012
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR DNase 1 from 202 using
DNAse1-Nde1-F
DNAse1-22
| lane |
3 |
4 |
| pcr |
1984 pL42 | |
| Nde1 +Xho1 | ||
Colony Screening
[edit]Date: 2014-1-17
Make miniprep and digest with Nde1 + Xho1
Result intepretation/Summary
[edit]Conclusion
[edit]Cloning of 2012-pL42-Dnase1
[edit]Purpose: Cloning of2012-pL42-Dnase1==
Date: 2014-7----
Notebook:0
Page:
Clone ID:2012
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR DNase 1 from 202 using
DNAse1-Nde1-F atcgac cat atg ctg aagatcgcagctttcaacatc
DNAse1-22 (5/3/2011)
(CACTAAGGTACCCTCGAGAATCC)
GGATTCTCGAGGGTACCTTAGTG
clone into 1984 pL42 Nde1 +Xho1
Colony Screening
[edit]Date: 2014_7
Make miniprep, use Nde1 +Xho1 to find positive clone
use PstI to verify
sent 2012-2 to sequence, missing one g
Cloning of 2012-pL42-Dnase1
[edit]Purpose: Cloning of2012-pL42-Dnase1==
Date: 2014-8----
Notebook:0
Page:
Clone ID:2012
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR DNase 1 from 2012-2 using
DNAse1-Nde1-F atcgac cat atg ctg aagatcgcagctttcaacatc
DNAse1-22 (5/3/2011)
(CACTAAGGTACCCTCGAGAATCC)
GGATTCTCGAGGGTACCTTAGTG
clone into 1984 pL42 Nde1 +Xho1
Colony Screening
[edit]Date: 2014_7
Make miniprep, use Nde1 +Xho1 to find positive clone
Result intepretation/Summary
[edit]Conclusion
[edit]