Editing 2959

T7AG-Flp

subclone from 2895 to 2953 using Nde1-Mlu1

[[File:2959 2960 digestion.JPG|RTENOTITLE]]

TAATACGACTCACTATAAGGtcttctggtccccacagactcagagagaacccatatgtaaGGCGCGCCCACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAATTGCCACCATGGCCCCCAAGAAAAAGCGGAAAGTGATGAGCCAGTTCGACATCCTGTGCAAGACCCCCCCCAAGGTGCTCGTGCGGCAGTTCGTGGAAAGATTCGAGAGGCCCAGCGGCGAGAAGATCGCCTCTTGTGCCGCCGAGCTGACCTACCTGTGCTGGATGATCACCCACAACGGCACCGCCATCAAGCGGGCCACCTTCATGAGCTACAACACCATCATCAGCAACAGCCTGAGCTTCGACATCGTGAACAAGAGCCTGCAGTTCAAGTACAAGACCCAGAAGGCCACCATCCTGGAAGCCAGCCTGAAGAAGCTGATCCCCGCCTGGGAGTTTACCATCATCCCCTACAACGGCCAGAAGCACCAGAGCGACATCACCGATATCGTGTCCAGCCTGCAGCTGCAGTTCGAGAGCAGCGAGGAAGCCGACAAGGGCAACAGCCACAGCAAGAAGATGCTGAAGGCCCTGCTGAGCGAGGGCGAGAGCATCTGGGAGATCACAGAGAAGATCCTGAACAGCTTCGAGTACACCAGCCGGTTCACCAAGACCAAGACCCTGTACCAGTTCCTGTTCCTGGCCACCTTTATCAACTGCGGCCGGTTCTCCGACATCAAGAACGTGGACCCCAAGAGCTTCAAGCTGGTGCAGAACAAGTACCTGGGCGTGATCATTCAGTGCCTCGTGACCGAGACAAAGACCAGCGTGTCCCGGCACATCTACTTTTTCAGCGCCAGAGGCCGGATCGACCCCCTGGTGTACCTGGACGAGTTCCTGAGAAACAGCGAGCCCGTGCTGAAGAGAGTGAACCGGACCGGCAACAGCAGCTCCAACAAGCAGGAATACCAGCTGCTGAAGGACAACCTCGTGCGGTCCTACAACAAGGCCCTGAAGAAAAACGCCCCCTACCCCATCTTCGCCATTAAGAACGGCCCCAAGTCCCACATCGGCCGGCACCTGATGACCAGCTTTCTGAGCATGAAGGGCCTGACAGAGCTGACCAACGTCGTGGGCAATTGGAGCGACAAGAGGGCCTCTGCCGTGGCCAGAACCACCTACACCCACCAGATCACAGCCATCCCCGACCACTACTTCGCCCTGGTGTCTCGGTACTACGCCTACGACCCCATCAGCAAAGAGATGATCGCCCTGAAGGACGAGACAAACCCCATCGAGGAATGGCAGCACATCGAGCAGCTGAAGGGCAGCGCCGAGGGCAGCATCAGATACCCTGCCTGGAACGGCATCATCTCCCAGGAAGTGCTGGACTACCTGAGCAGCTACATCAACCGGCGGATCTAGCGGCCGCTTAAGCTGCTTACCGCAAGCTGCTTACATAGAACTCGCGGCGATTGGCATGCCCGCCTTAAAATTTTTATTTTATTTTTtCTTTTCTTTTCCGAATCGGATTTTGTTTTTAATATTTCAAAAAAAAAAAAAAAAAAAAAAAAACGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAttaatAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAttaatAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAttaattaatctagaacgcgTCGAGGGGAATTAATTCTTGAAGACGAAAGGGCCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGAGCTC
<pre>>A220401-011_M22_1FC0DAA006_premix.ab1	1231 4/2/2022
2959 T7AG-Flp
sequencing using T7AG


GNNNNNNNNANNCNNNTNNNNGATGAGACNTCTTNANATATTATTGTCAGAGCTCCCTGTAGGTGCTTTAGCGGGACGCTATGTCCGGATTCTGATGTAC
CTGCTGAGGTAATATAGCCTTTTGTGGGAGATAATTGAGTTACATGTGCGGGATGAGATGCTGCCCTCGGCCCTGCCCTTCCGGGCTCCTTCNCTGCCAT
TCCTCCATGGTCTTTGTCTCGCTGATCTTCGACATCATGTATTTGATGATGGGGTTCAAGGACAAGACCCAAAACGCCACCATCCTGNAAGGGTCGGGAA
TGGNTGGATCCTGGCGTGGGAGTTTGCCTTCGTCCCCTACAACGGCCCTATTGTCCAGAGCGTTGTCACCGATATCGTGTCCAGCCTGCAGCTGCAGTTC
GAGAGCAGCGAGGAAGCCCACGAGGGCAACAGCCACAGCAAGAAGATGCT
GAATGCCCTGCTGAGCGAGGGCGAGAGCATCTGGGATATCACAGAGAAGA
TCCTGAACAGCTTCGAGTACACCAGCCGGTTCACCAAGACCAAGACCCTG
TACCAGTTCCTGTTCCTGGCCACCTTTATCAACTGCGGCCGGTTCTCCNA
CATCAAGAACGTGGACCCCAAGAGCTTCAAGCTGGTGCAGAACAAGTACC
TGGGCGTGATCATTCAGTGNCTCGTGACCGANACATAGACCAGCGTGTCC
CGGCNCATCTACTTTTTCAGCGCCAGANGNCGGATCGACCCCCTGGTGTA
CCTGGNCNAGTTCCTGAGAACAGCGAGCCCGTGCTGAANAGAGTGAACCG
GACCGGCAACAGCANCTGCNACAAGCANGANTACCAGCTGCNTGANGGAC
NATCTCNTGNNGGTCTNTACANNATGTCTCNTGANNAAAACGCCCCACTN
ACNCNTCTTCGCCATTAANANGGCCCAAGTCCNNACATCGGCGGCACTGA
TGACCAGNTTTCNNAGCATGANNGNTGANNAGCTGACNNCGTCNTGGGTC
AATTGNNTCNACANANNNNTNTNNNTNNNNNANNNTNNNNNNCNNATNNN
NGCATCNCGACNNCTACTTNNCNTGNGNNNCNGNACTACNCNACGACCNT
NNGNNNNNNNGATNNNNNAANNNNANNNANNNNNTNNGGATGNAGCNANT
NGNNNNTNANNNNGNCNNNNGNATNNNNNNNNCNNNNGNNNNNNNNNNNT
NTNNNTNNNNNNCNNNNNANNNNNCNNNNNN

Alignment statistics for match #1
Score	Expect	Identities	Gaps	Strand
920 bits(498)	0.0	642/726(88%)	15/726(2%)	Plus/Plus
Query  254   GTTCAAGGACAAGACCCAAAACGCCACCATCCTGNAAG-GGTCGGGAATG-GNTGGATCC  311
             ||||||| |||||||||| || |||||||||||| |||    |  |||   | | |||||
Sbjct  383   GTTCAAGTACAAGACCCAGAAGGCCACCATCCTGGAAGCCAGCCTGAAGAAGCT-GATCC  441

Query  312   TGGCGTGGGAGTTTGCCTTCGTCCCCTACAACGGCCCTATTGTCCAGAGCGTTGTCACCG  371
               || ||||||||| || || |||||||||||||||  |    ||||||||   ||||||
Sbjct  442   CCGCCTGGGAGTTTACCATCATCCCCTACAACGGCCAGAAGCACCAGAGCGACATCACCG  501

Query  372   ATATCGTGTCCAGCCTGCAGCTGCAGTTCGAGAGCAGCGAGGAAGCCCACGAGGGCAACA  431
             ||||||||||||||||||||||||||||||||||||||||||||||| || |||||||||
Sbjct  502   ATATCGTGTCCAGCCTGCAGCTGCAGTTCGAGAGCAGCGAGGAAGCCGACAAGGGCAACA  561

Query  432   GCCACAGCAAGAAGATGCTGAATGCCCTGCTGAGCGAGGGCGAGAGCATCTGGGATATCA  491
             |||||||||||||||||||||| |||||||||||||||||||||||||||||||| ||||
Sbjct  562   GCCACAGCAAGAAGATGCTGAAGGCCCTGCTGAGCGAGGGCGAGAGCATCTGGGAGATCA  621

Query  492   CAGAGAAGATCCTGAACAGCTTCGAGTACACCAGCCGGTTCACCAAGACCAAGACCCTGT  551
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  622   CAGAGAAGATCCTGAACAGCTTCGAGTACACCAGCCGGTTCACCAAGACCAAGACCCTGT  681

Query  552   ACCAGTTCCTGTTCCTGGCCACCTTTATCAACTGCGGCCGGTTCTCCNACATCAAGAACG  611
             ||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
Sbjct  682   ACCAGTTCCTGTTCCTGGCCACCTTTATCAACTGCGGCCGGTTCTCCGACATCAAGAACG  741

Query  612   TGGACCCCAAGAGCTTCAAGCTGGTGCAGAACAAGTACCTGGGCGTGATCATTCAGTGNC  671
             |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
Sbjct  742   TGGACCCCAAGAGCTTCAAGCTGGTGCAGAACAAGTACCTGGGCGTGATCATTCAGTGCC  801

Query  672   TCGTGACCGANACATAGACCAGCGTGTCCCGGCNCATCTACTTTTTCAGCGCCAGANGNC  731
             |||||||||| ||| |||||||||||||||||| |||||||||||||||||||||| | |
Sbjct  802   TCGTGACCGAGACAAAGACCAGCGTGTCCCGGCACATCTACTTTTTCAGCGCCAGAGGCC  861

Query  732   GGATCGACCCCCTGGTGTACCTGGNCNAGTTCCTGAG-AACAGCGAGCCCGTGCTGAANA  790
             |||||||||||||||||||||||| | |||||||||| |||||||||||||||||||| |
Sbjct  862   GGATCGACCCCCTGGTGTACCTGGACGAGTTCCTGAGAAACAGCGAGCCCGTGCTGAAGA  921

Query  791   GAGTGAACCGGACCGGCAACAGCANCTGCNACAAGCANGANTACCAGCTGCNTGANGGAC  850
             |||||||||||||||||||||||| || | ||||||| || |||||||||| ||| ||||
Sbjct  922   GAGTGAACCGGACCGGCAACAGCAGCTCCAACAAGCAGGAATACCAGCTGC-TGAAGGAC  980

Query  851   NATCTCNTGNNGGTCTNTACANNATGTCTCNTGANNAAAA-CGCCCCACTNACNCNTCTT  909
              | ||| ||  ||||  ||||  | | | | |||  |||| |||||| ||  | | ||||
Sbjct  981   AACCTCGTGC-GGTCC-TACAACAAGGCCC-TGAAGAAAAACGCCCC-CTACCCCATCTT  1036

Query  910   CGCCATTAANAN-GGCCC-AAGTCCNNACATCGGC-GGCA-CTGATGACCAGNTTTCNNA  965
             ||||||||| |  ||||| ||||||  |||||||| |||| ||||||||||| ||||  |
Sbjct  1037  CGCCATTAAGAACGGCCCCAAGTCCC-ACATCGGCCGGCACCTGATGACCAGCTTTCTGA  1095

Query  966   GCATGA  971
             ||||||
Sbjct  1096  GCATGA  1101

</pre>
[[Category:Cloning]]