2959
T7AG-Flp
subclone from 2895 to 2953 using Nde1-Mlu1
TAATACGACTCACTATAAGGtcttctggtccccacagactcagagagaacccatatgtaaGGCGCGCCCACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAATTGCCACCATGGCCCCCAAGAAAAAGCGGAAAGTGATGAGCCAGTTCGACATCCTGTGCAAGACCCCCCCCAAGGTGCTCGTGCGGCAGTTCGTGGAAAGATTCGAGAGGCCCAGCGGCGAGAAGATCGCCTCTTGTGCCGCCGAGCTGACCTACCTGTGCTGGATGATCACCCACAACGGCACCGCCATCAAGCGGGCCACCTTCATGAGCTACAACACCATCATCAGCAACAGCCTGAGCTTCGACATCGTGAACAAGAGCCTGCAGTTCAAGTACAAGACCCAGAAGGCCACCATCCTGGAAGCCAGCCTGAAGAAGCTGATCCCCGCCTGGGAGTTTACCATCATCCCCTACAACGGCCAGAAGCACCAGAGCGACATCACCGATATCGTGTCCAGCCTGCAGCTGCAGTTCGAGAGCAGCGAGGAAGCCGACAAGGGCAACAGCCACAGCAAGAAGATGCTGAAGGCCCTGCTGAGCGAGGGCGAGAGCATCTGGGAGATCACAGAGAAGATCCTGAACAGCTTCGAGTACACCAGCCGGTTCACCAAGACCAAGACCCTGTACCAGTTCCTGTTCCTGGCCACCTTTATCAACTGCGGCCGGTTCTCCGACATCAAGAACGTGGACCCCAAGAGCTTCAAGCTGGTGCAGAACAAGTACCTGGGCGTGATCATTCAGTGCCTCGTGACCGAGACAAAGACCAGCGTGTCCCGGCACATCTACTTTTTCAGCGCCAGAGGCCGGATCGACCCCCTGGTGTACCTGGACGAGTTCCTGAGAAACAGCGAGCCCGTGCTGAAGAGAGTGAACCGGACCGGCAACAGCAGCTCCAACAAGCAGGAATACCAGCTGCTGAAGGACAACCTCGTGCGGTCCTACAACAAGGCCCTGAAGAAAAACGCCCCCTACCCCATCTTCGCCATTAAGAACGGCCCCAAGTCCCACATCGGCCGGCACCTGATGACCAGCTTTCTGAGCATGAAGGGCCTGACAGAGCTGACCAACGTCGTGGGCAATTGGAGCGACAAGAGGGCCTCTGCCGTGGCCAGAACCACCTACACCCACCAGATCACAGCCATCCCCGACCACTACTTCGCCCTGGTGTCTCGGTACTACGCCTACGACCCCATCAGCAAAGAGATGATCGCCCTGAAGGACGAGACAAACCCCATCGAGGAATGGCAGCACATCGAGCAGCTGAAGGGCAGCGCCGAGGGCAGCATCAGATACCCTGCCTGGAACGGCATCATCTCCCAGGAAGTGCTGGACTACCTGAGCAGCTACATCAACCGGCGGATCTAGCGGCCGCTTAAGCTGCTTACCGCAAGCTGCTTACATAGAACTCGCGGCGATTGGCATGCCCGCCTTAAAATTTTTATTTTATTTTTtCTTTTCTTTTCCGAATCGGATTTTGTTTTTAATATTTCAAAAAAAAAAAAAAAAAAAAAAAAACGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAttaatAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAttaatAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAttaattaatctagaacgcgTCGAGGGGAATTAATTCTTGAAGACGAAAGGGCCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGAGCTC
>A220401-011_M22_1FC0DAA006_premix.ab1 1231 4/2/2022
2959 T7AG-Flp
sequencing using T7AG
GNNNNNNNNANNCNNNTNNNNGATGAGACNTCTTNANATATTATTGTCAGAGCTCCCTGTAGGTGCTTTAGCGGGACGCTATGTCCGGATTCTGATGTAC
CTGCTGAGGTAATATAGCCTTTTGTGGGAGATAATTGAGTTACATGTGCGGGATGAGATGCTGCCCTCGGCCCTGCCCTTCCGGGCTCCTTCNCTGCCAT
TCCTCCATGGTCTTTGTCTCGCTGATCTTCGACATCATGTATTTGATGATGGGGTTCAAGGACAAGACCCAAAACGCCACCATCCTGNAAGGGTCGGGAA
TGGNTGGATCCTGGCGTGGGAGTTTGCCTTCGTCCCCTACAACGGCCCTATTGTCCAGAGCGTTGTCACCGATATCGTGTCCAGCCTGCAGCTGCAGTTC
GAGAGCAGCGAGGAAGCCCACGAGGGCAACAGCCACAGCAAGAAGATGCT
GAATGCCCTGCTGAGCGAGGGCGAGAGCATCTGGGATATCACAGAGAAGA
TCCTGAACAGCTTCGAGTACACCAGCCGGTTCACCAAGACCAAGACCCTG
TACCAGTTCCTGTTCCTGGCCACCTTTATCAACTGCGGCCGGTTCTCCNA
CATCAAGAACGTGGACCCCAAGAGCTTCAAGCTGGTGCAGAACAAGTACC
TGGGCGTGATCATTCAGTGNCTCGTGACCGANACATAGACCAGCGTGTCC
CGGCNCATCTACTTTTTCAGCGCCAGANGNCGGATCGACCCCCTGGTGTA
CCTGGNCNAGTTCCTGAGAACAGCGAGCCCGTGCTGAANAGAGTGAACCG
GACCGGCAACAGCANCTGCNACAAGCANGANTACCAGCTGCNTGANGGAC
NATCTCNTGNNGGTCTNTACANNATGTCTCNTGANNAAAACGCCCCACTN
ACNCNTCTTCGCCATTAANANGGCCCAAGTCCNNACATCGGCGGCACTGA
TGACCAGNTTTCNNAGCATGANNGNTGANNAGCTGACNNCGTCNTGGGTC
AATTGNNTCNACANANNNNTNTNNNTNNNNNANNNTNNNNNNCNNATNNN
NGCATCNCGACNNCTACTTNNCNTGNGNNNCNGNACTACNCNACGACCNT
NNGNNNNNNNGATNNNNNAANNNNANNNANNNNNTNNGGATGNAGCNANT
NGNNNNTNANNNNGNCNNNNGNATNNNNNNNNCNNNNGNNNNNNNNNNNT
NTNNNTNNNNNNCNNNNNANNNNNCNNNNNN
Alignment statistics for match #1
Score Expect Identities Gaps Strand
920 bits(498) 0.0 642/726(88%) 15/726(2%) Plus/Plus
Query 254 GTTCAAGGACAAGACCCAAAACGCCACCATCCTGNAAG-GGTCGGGAATG-GNTGGATCC 311
||||||| |||||||||| || |||||||||||| ||| | ||| | | |||||
Sbjct 383 GTTCAAGTACAAGACCCAGAAGGCCACCATCCTGGAAGCCAGCCTGAAGAAGCT-GATCC 441
Query 312 TGGCGTGGGAGTTTGCCTTCGTCCCCTACAACGGCCCTATTGTCCAGAGCGTTGTCACCG 371
|| ||||||||| || || ||||||||||||||| | |||||||| ||||||
Sbjct 442 CCGCCTGGGAGTTTACCATCATCCCCTACAACGGCCAGAAGCACCAGAGCGACATCACCG 501
Query 372 ATATCGTGTCCAGCCTGCAGCTGCAGTTCGAGAGCAGCGAGGAAGCCCACGAGGGCAACA 431
||||||||||||||||||||||||||||||||||||||||||||||| || |||||||||
Sbjct 502 ATATCGTGTCCAGCCTGCAGCTGCAGTTCGAGAGCAGCGAGGAAGCCGACAAGGGCAACA 561
Query 432 GCCACAGCAAGAAGATGCTGAATGCCCTGCTGAGCGAGGGCGAGAGCATCTGGGATATCA 491
|||||||||||||||||||||| |||||||||||||||||||||||||||||||| ||||
Sbjct 562 GCCACAGCAAGAAGATGCTGAAGGCCCTGCTGAGCGAGGGCGAGAGCATCTGGGAGATCA 621
Query 492 CAGAGAAGATCCTGAACAGCTTCGAGTACACCAGCCGGTTCACCAAGACCAAGACCCTGT 551
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 622 CAGAGAAGATCCTGAACAGCTTCGAGTACACCAGCCGGTTCACCAAGACCAAGACCCTGT 681
Query 552 ACCAGTTCCTGTTCCTGGCCACCTTTATCAACTGCGGCCGGTTCTCCNACATCAAGAACG 611
||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
Sbjct 682 ACCAGTTCCTGTTCCTGGCCACCTTTATCAACTGCGGCCGGTTCTCCGACATCAAGAACG 741
Query 612 TGGACCCCAAGAGCTTCAAGCTGGTGCAGAACAAGTACCTGGGCGTGATCATTCAGTGNC 671
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
Sbjct 742 TGGACCCCAAGAGCTTCAAGCTGGTGCAGAACAAGTACCTGGGCGTGATCATTCAGTGCC 801
Query 672 TCGTGACCGANACATAGACCAGCGTGTCCCGGCNCATCTACTTTTTCAGCGCCAGANGNC 731
|||||||||| ||| |||||||||||||||||| |||||||||||||||||||||| | |
Sbjct 802 TCGTGACCGAGACAAAGACCAGCGTGTCCCGGCACATCTACTTTTTCAGCGCCAGAGGCC 861
Query 732 GGATCGACCCCCTGGTGTACCTGGNCNAGTTCCTGAG-AACAGCGAGCCCGTGCTGAANA 790
|||||||||||||||||||||||| | |||||||||| |||||||||||||||||||| |
Sbjct 862 GGATCGACCCCCTGGTGTACCTGGACGAGTTCCTGAGAAACAGCGAGCCCGTGCTGAAGA 921
Query 791 GAGTGAACCGGACCGGCAACAGCANCTGCNACAAGCANGANTACCAGCTGCNTGANGGAC 850
|||||||||||||||||||||||| || | ||||||| || |||||||||| ||| ||||
Sbjct 922 GAGTGAACCGGACCGGCAACAGCAGCTCCAACAAGCAGGAATACCAGCTGC-TGAAGGAC 980
Query 851 NATCTCNTGNNGGTCTNTACANNATGTCTCNTGANNAAAA-CGCCCCACTNACNCNTCTT 909
| ||| || |||| |||| | | | | ||| |||| |||||| || | | ||||
Sbjct 981 AACCTCGTGC-GGTCC-TACAACAAGGCCC-TGAAGAAAAACGCCCC-CTACCCCATCTT 1036
Query 910 CGCCATTAANAN-GGCCC-AAGTCCNNACATCGGC-GGCA-CTGATGACCAGNTTTCNNA 965
||||||||| | ||||| |||||| |||||||| |||| ||||||||||| |||| |
Sbjct 1037 CGCCATTAAGAACGGCCCCAAGTCCC-ACATCGGCCGGCACCTGATGACCAGCTTTCTGA 1095
Query 966 GCATGA 971
||||||
Sbjct 1096 GCATGA 1101