Editing 553-567

== '''Cloning of 553-567'''  ==

'''Purpose:''' Cloning of 553-567 

'''Date: '''2012-9-14---2012-9-20<br> 

'''Notebook:&nbsp;''' 

'''Page:''' 

'''Clone ID: '''553---567 

'''Experimenters: '''yingying lu 

== '''Results'''  ==

=== Cloning<br>  ===

Date: 2012-9-14 

[[Image:553+560+561+563+564+566 PCR 9-14-2012.JPG]] 

[[Image:554+557+558+559+562+565+567 PCR1+2 9-14-2012.JPG]] 

[[Image:554+557+558+559+562+565+567 PCR 9-17-2012.JPG]] 

<br> 

2012-9-18<br> 

clone 553(lane1)-567(lane13):Nde1+Xho1; plasmid 1254 as vector<br> 

lane1 and 10 smear, so did not do the clone 553 and 564 

[[Image:554-567 clone 9-19-2012.JPG]][[Image:554-567 vectror 9-19-2012.JPG]] 

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=== <u></u>Colony Screening<br>  ===

2012-9-20 

mini prep and digest with&nbsp;:Nde1+Xho1<br> 

no insert 

<br> 

<br> 

<br> 

== '''Result intepretation/Summary'''  ==

<br> 

== '''Conclusion'''  ==

<br> 

== '''Cloning of 559-pET30b.Amp-Not1,567-pET30b.Amp-Not1'''  ==

'''Purpose:''' Cloning of 559-pET30b.Amp-Not1,567-pET30b.Amp-Not1 

'''Date: '''2012-10-9---2012-10-11 

'''Notebook:0''' 

'''Page:''' 

'''Clone ID:559,567''' 

'''Experimenters:''' yingying 

== '''Results'''  ==

=== cloning  ===

[[Image:559+567+1097+1411 clone 10-9-2012.JPG]]<br> 

<br> 

{| cellspacing="1" cellpadding="1" border="1" style="width: 281px; height: 66px;"
|-
| <br> 
| band 1,3<br> 
| band 2,4<br>
|-
| <br> 
| PCR product<br> 
| pET30b.Amp<br>
|-
| enzymes<br> 
| colspan="2" | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Nde1 + Xho1.<br>
|}

<br> 

{| cellspacing="1" cellpadding="1" border="1"
|-
| <br>
|}

=== Colony Screening  ===

Date: 2012-10-11 

Make miniprep and digest with Nde1 + Xho1. 

[[Image:559+567 digest detect 10-11-2012.JPG]]<br> 

no insert ,only the vector self ligate 

c <br> 

== '''Result intepretation/Summary'''  ==

*&amp;lt;br&amp;gt;

== '''Conclusion'''  ==

&amp;lt;br&amp;gt; 

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== '''Cloning of 559-pET30b.Amp-Not1,567-pET30b.Amp-Not1'''  ==

'''Purpose:''' Cloning of 559-pET30b.Amp-Not1,567-pET30b.Amp-Not1 

'''Date: '''2012-10-15---2012-10-18 

'''Notebook:0''' 

'''Page:''' 

'''Clone ID:559,567''' 

'''Experimenters:''' yingying 

== '''Results'''  ==

=== cloning  ===

[[Image:240+559+567+1248 clone 10-16-2012.JPG]]<br> 

{| width="356" cellspacing="1" cellpadding="1" border="1"
|-
| <br> 
| band 3,5<br> 
| band 4,6<br>
|-
| <br> 
| PCR product<br> 
| 1026,pET28b-LambdaExo<br>
|-
| enzymes<br> 
| colspan="2" | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Nde1 + Xho1.<br>
|}

<br> 

{| cellspacing="1" cellpadding="1" border="1"
|-
| <br>
|}

=== Colony Screening  ===

Date: 2012-10-18 

Make miniprep and digest with Nde1 + Xho1. 

[[Image:559+567+1248 digest detect 10-18-2012.JPG]]<br> 

559clone 5,6,7,8 may be right , 

567 clone 4 may be right,<br> 

c&nbsp;:1026pET28b-LambdaExo digested with Nde1 + Xho1<br> 

== '''Result intepretation/Summary'''  ==

*&amp;lt;br&amp;gt;

== '''Conclusion'''  ==

&amp;lt;br&amp;gt; 

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== '''Cloning of 554-pET28b-Nde1,560-pET28b-Kpn1'''  ==

'''Purpose:''' Cloning of pET28b-Nde1,560-pET28b-Kpn1 

'''Date: '''2013-2-13---2013-2-19

'''Notebook:0''' 

'''Page:''' 

'''Clone ID:554,560''' 

'''Experimenters:''' yingying 

== '''Results'''  ==

=== cloning  ===

[[Image:554+560+1540 clone 2-14-2013.JPG]]<br> 

{| width="356" cellspacing="1" cellpadding="1" border="1"
|-
| <br> 
| band 1,3<br> 
| band 2,4<br>
|-
| <br> 
| PCR product<br> 
| ,pET28b<br>
|-
| enzymes<br> 
| colspan="2" | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Nde1 + Xho1.<br>
|}

<br> 

{| cellspacing="1" cellpadding="1" border="1"
|-
| <br>
|}

=== Colony Screening  ===

Date: 2013-2-19 

Make miniprep and digest with Nde1 + Xho1. 

[[Image:554+560_digest_detect_2-19-2013.JPG]][[Image:554+1540_digest_detect_2-20-2013.JPG]]<br> 

<br> 

== '''Result intepretation/Summary'''  ==

*&amp;lt;br&amp;gt;

== '''Conclusion'''  ==

&amp;lt;br&amp;gt; 

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&amp;lt;br&amp;gt; 

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[[Category:Cloning]]