553-567
Cloning of 553-567
[edit]Purpose: Cloning of 553-567
Date: 2012-9-14---2012-9-20
Notebook:
Page:
Clone ID: 553---567
Experimenters: yingying lu
Results
[edit]Cloning
[edit]Date: 2012-9-14
2012-9-18
clone 553(lane1)-567(lane13):Nde1+Xho1; plasmid 1254 as vector
lane1 and 10 smear, so did not do the clone 553 and 564
Colony Screening
[edit]2012-9-20
mini prep and digest with :Nde1+Xho1
no insert
Result intepretation/Summary
[edit]
Conclusion
[edit]
Cloning of 559-pET30b.Amp-Not1,567-pET30b.Amp-Not1
[edit]Purpose: Cloning of 559-pET30b.Amp-Not1,567-pET30b.Amp-Not1
Date: 2012-10-9---2012-10-11
Notebook:0
Page:
Clone ID:559,567
Experimenters: yingying
Results
[edit]cloning
[edit]
| band 1,3 |
band 2,4 | |
| PCR product |
pET30b.Amp | |
| enzymes |
Nde1 + Xho1. | |
Colony Screening
[edit]Date: 2012-10-11
Make miniprep and digest with Nde1 + Xho1.
no insert ,only the vector self ligate
c
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>
<br>
<br>
Cloning of 559-pET30b.Amp-Not1,567-pET30b.Amp-Not1
[edit]Purpose: Cloning of 559-pET30b.Amp-Not1,567-pET30b.Amp-Not1
Date: 2012-10-15---2012-10-18
Notebook:0
Page:
Clone ID:559,567
Experimenters: yingying
Results
[edit]cloning
[edit]
| band 3,5 |
band 4,6 | |
| PCR product |
1026,pET28b-LambdaExo | |
| enzymes |
Nde1 + Xho1. | |
Colony Screening
[edit]Date: 2012-10-18
Make miniprep and digest with Nde1 + Xho1.
559clone 5,6,7,8 may be right ,
567 clone 4 may be right,
c :1026pET28b-LambdaExo digested with Nde1 + Xho1
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>
<br>
<br>
Cloning of 554-pET28b-Nde1,560-pET28b-Kpn1
[edit]Purpose: Cloning of pET28b-Nde1,560-pET28b-Kpn1
Date: 2013-2-13---2013-2-19
Notebook:0
Page:
Clone ID:554,560
Experimenters: yingying
Results
[edit]cloning
[edit]
| band 1,3 |
band 2,4 | |
| PCR product |
,pET28b | |
| enzymes |
Nde1 + Xho1. | |
Colony Screening
[edit]Date: 2013-2-19
Make miniprep and digest with Nde1 + Xho1.
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>
<br>
<br>