Editing 6 mRNA Stefania

2012-7-17 

[[Image:1259 PCR 7-18-2012.JPG|376x213px|1259 PCR 7-18-2012.JPG]][[Image:1260+1261 PCR 7-18-2012.JPG|181x211px|1260+1261 PCR 7-18-2012.JPG]] 

1259 and 1260 right size 

<br> 

2012-7-18 

[[Image:1259 PCR 7-18-2012.JPG|374x212px|1259 PCR 7-18-2012.JPG]][[Image:1260 PCR 7-18-2012.JPG|178x208px|1260 PCR 7-18-2012.JPG]] 

1259 and 1260 right 

<br> 

2012-7-20<br> 

digest detect&nbsp;: EcoR1+Xho1 

{| cellspacing="1" cellpadding="1" border="1" style="width: 425px; height: 117px;"
|-
| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Lane 
| 1 
| 2 
| 3 
| 4 
| 5 
| 6
|-
| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; ID 
| 1256 
| 1257 
| 1258 
| 1259 
| 1260 
| 1261
|-
| insert size should be(bp) 
| &nbsp;996 
| 1251 
| 1926 
| 2196 
| 360 
| 1497
|}

<br> 

[[Image:1256-1261 digest detec 7-20-2012.JPG|252x279px|1256-1261 digest detec 7-20-2012.JPG]][[Image:1256 digest .JPG|245x282px|1256 digest .JPG]]<br> 

<br> 

2012-7-23 

&nbsp;diges again 

1----1256 

2-----1259 

3----1261 

[[Image:1256 digest 7-23-2012.JPG|192x267px|1256 digest 7-23-2012.JPG]] 

<br> 

2012-7-24 

plasmid QC 

[[Image:Plasmid for RNA QC 7-24-2012.JPG|221x258px|Plasmid for RNA QC 7-24-2012.JPG]] 

{| width="200" cellspacing="1" cellpadding="1" border="1"
|-
| lane 
| 1 
| 2 
| 3 
| 4 
| 5 
| 6
|-
| ID 
| 1256 
| 1257 
| 1258 
| 1259 
| 1260 
| 
1261 

|}

<br> 

2012-7-24 

PCR again in 2.5%DMSO: 1256 to 1261 

[[Image:1256-1261 PCR 7-24-2012.JPG|281x194px|1256-1261 PCR 7-24-2012.JPG]] 

<br> 

<br> 

2012-7-27 

new primers 

[[Image:1257+1257+1258+1259 PCR 7-27-2012.JPG|422x251px|1257+1257+1258+1259 PCR 7-27-2012.JPG]][[Image:1260+1261 PCR 7-27-2012.JPG|220x251px|1260+1261 PCR 7-27-2012.JPG]] 

1261 no lane 

<br> 

2012-8-8 

1327 new clones replace 1261 



2012-8-28

[[Image:1256_PCR_8-28-2012.JPG]]

1256 ?

[[Image:1257+1258+1259+1260+1261_pcr_8-28-2012.JPG]]

<br> 

<br> 

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