6 mRNA Stefania

2012-7-17

1259 and 1260 right size

2012-7-18

1259 and 1260 right

2012-7-20

digest detect : EcoR1+Xho1

Lane	1	2	3	4	5	6
ID	1256	1257	1258	1259	1260	1261
insert size should be(bp)	996	1251	1926	2196	360	1497

2012-7-23

 diges again

1----1256

2-----1259

3----1261

2012-7-24

plasmid QC

lane	1	2	3	4	5	6
ID	1256	1257	1258	1259	1260	1261

2012-7-24

PCR again in 2.5%DMSO: 1256 to 1261

2012-7-27

new primers

1261 no lane

2012-8-8

1327 new clones replace 1261

2012-8-28

1256 ?