Editing 7 mRNA Stefania

mRNA Production for Stefania 

6/8/2012 

PCR amplification using plasmids from Stefania 

[[Image:1211 PCR 6-5-2012.JPG]] 

[[Image:1212 1215 1216 PCR 6-8-2012.JPG]] 

[[Image:1209 1212 1214 1215 1216 PCR 6-8-2012.JPG]] 

[[Image:1210 1213 PCR 6-8-2012.JPG]] 

<br> 

2012/6/11 

1209---1216 digest result:<br> 

{| cellspacing="1" cellpadding="1" border="1" style="width: 689px; height: 80px;"
|-
| 
Lane<br> 

| 1<br> 
| 5<br> 
| 10<br> 
| 2<br> 
| 8<br> 
| 3<br> 
| 11<br> 
| 4<br> 
| 15<br>
|-
| enzymes<br> 
| xba1+Not1<br> 
| xba1<br> 
| xba1+sal1<br> 
| xba1+sal1<br> 
| xba1<br> 
| EcoR1+Pst1<br> 
| xba1+sal1<br> 
| xba1<br> 
| xba1+sal1<br>
|-
| ID<br> 
| colspan="3" | 1209pMXs-Lhx3<br> 
| colspan="2" | 1214pMXs-Isl1<br> 
| colspan="2" | 1210Tet-O-FUW-FoxG<br> 
| colspan="2" | 1216pMXs-Brn2<br>
|}

{| cellspacing="1" cellpadding="1" border="1" style="width: 622px; height: 80px;"
|-
| Lane 
| 6<br> 
| 12<br> 
| 7<br> 
| 13<br> 
| 9<br> 
| 14<br> 
| <br>
|-
| enzymes<br> 
| xba1<br> 
| xba1+sal1<br> 
| xba1<br> 
| EcoR1+xba1<br> 
| xba1<br> 
| xba1+sal1<br> 
| <br>
|-
| ID<br> 
| colspan="2" | 1211pMXs-MYT1L<br> 
| 1212pMXs-Ngn2<br> 
| 1213pCS2-MT GLI2 FL<br> 
| colspan="2" | 1215pMXs-Hb9<br> 
| <br>
|}

[[Image:1209--1216 digest 6-11-2012.JPG]]<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <br> 2012/6/12 PCR: 

[[Image:1211 1212 1213 1215 1216 PCR 6-12-2012.JPG]] 

1211,1212,1215,1216 sizes are right 

[[Image:1213PCR 6 13 2012.JPG]] 

1213 size right<br> 

[[Image:1209 1210 1211 1212PCR 6-13-2012.JPG]] 

1209,1211 size right 

[[Image:1213 1214 1215 1216PCR 6-14-2012.JPG]] 

1214 size right 

[[Image:1210 1212 1215 1216PCR 6-14-2012.JPG]] 

1216 size right 

[[Image:1210_1212_1215_1216PCR_6_18-2012.JPG]]

size are right

<br> 

Clones replacement: 

pMXs-Brn2 ==&gt;pCMV6-POU3F2<br>pGEM-ISLET1==&gt;pMXs-Isl1<br> 

BHLHB9: no growth<br> <br> 

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