7 mRNA Stefania
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mRNA Production for Stefania
6/8/2012
PCR amplification using plasmids from Stefania
2012/6/11
1209---1216 digest result:
|
Lane |
1 |
5 |
10 |
2 |
8 |
3 |
11 |
4 |
15 |
| enzymes |
xba1+Not1 |
xba1 |
xba1+sal1 |
xba1+sal1 |
xba1 |
EcoR1+Pst1 |
xba1+sal1 |
xba1 |
xba1+sal1 |
| ID |
1209pMXs-Lhx3 |
1214pMXs-Isl1 |
1210Tet-O-FUW-FoxG |
1216pMXs-Brn2 | |||||
| Lane | 6 |
12 |
7 |
13 |
9 |
14 |
|
| enzymes |
xba1 |
xba1+sal1 |
xba1 |
EcoR1+xba1 |
xba1 |
xba1+sal1 |
|
| ID |
1211pMXs-MYT1L |
1212pMXs-Ngn2 |
1213pCS2-MT GLI2 FL |
1215pMXs-Hb9 |
|||
2012/6/12 PCR:
1211,1212,1215,1216 sizes are right
1213 size right
1209,1211 size right
1214 size right
1216 size right
size are right
Clones replacement:
pMXs-Brn2 ==>pCMV6-POU3F2
pGEM-ISLET1==>pMXs-Isl1
BHLHB9: no growth