Editing 948 pJet1.2-ccdb

948, not 947 digest with EcoRV

[[File:948 EcoRV 2.JPG|RTENOTITLE]]

[[File:948 EcoRV.JPG|RTENOTITLE]]



[[File:948 Xho1 Xba1 950 Xho1.JPG|RTENOTITLE]]



= == =

Date 1/22/2018

Digest miniprep DNA (in pJect1.2 vector) with Xho1-Xba1, (Cloning sites Xho1-Xba1)

[[File:3001 3009 3135 950 948 digestion.JPG|RTENOTITLE]]

[[File:948-3135-3009-3001-950 digestion2.JPG.JPG|RTENOTITLE]]



948 vector, digest with EcoRV&nbsp;+&nbsp; CIP

ligate with PCR product, make miniprep, digest with Xba1+ Xho1

[[File:948 cloning CIP EcoRV.JPG]]

[[File:948 ligation.jpg]]