948 pJet1.2-ccdb

948, not 947 digest with EcoRV

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Date 1/22/2018

Digest miniprep DNA (in pJect1.2 vector) with Xho1-Xba1, (Cloning sites Xho1-Xba1)

948 vector, digest with EcoRV +  CIP

ligate with PCR product, make miniprep, digest with Xba1+ Xho1