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Urgent Tasks {| border="1" cellspacing="1" cellpadding="1" style="width:500px;" |- | ID | Clone Name | Product Name | Note | Order ID | Cat # |- | 162<br/> | pCold-MBP-(Tev)-GST-(3C)-Tat-NLS-iFLP-6H<br/> | Flp | <br/> | 5148<br/> | EG-1002 |- | 2108 | pCold-Tat-NLS-iFLP-6H<br/> | Flp | <br/> | 5148<br/> | EG-1002<br/> |- | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> |- | 2503<br/> | pCold-GST-VVTopo<br/> | Vaccinia Virus Topoisomerase I<br/> | <br/> | 5161 5154 | EG-167<br/> |- | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> |- | 2291 | pT7A3-SortaseAM-N6H | Bifunctional Protein Ligase/Protease Sortase A<br/> | [http://www.excellgen.net/eln/index.php/212_429_2238_2197_2249_2287_2232_2291_2290_2294 expressed] | 5159<br/> | EG-42<br/> |- | 2232 <br/> | pT7A3-SortaseAM-C6H<br/> | Bifunctional Protein Ligase/Protease Sortase A<br/> | [http://www.excellgen.net/eln/index.php/212_429_2238_2197_2249_2287_2232_2291_2290_2294 no expression] | 5159<br/> | EG-42<br/> |- | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> |} {| border="1" cellspacing="1" cellpadding="1" style="width:500px;" |- | '''ID''' | Status | '''Name''' | '''DNA Size''' | '''Vector''' | '''Method''' | '''Progress'''<br/> | '''Date''' | '''Enzyme'''<br/> | '''Expression''' | '''Note''' |- | <span style="color:#FF0000;">3127</span><br/> | <br/> | <span style="color:#FF0000;">pET28b-qBeta-qBeta-6XHis</span><br/> | <br/> | <br/> | <br/> | <span style="color:#FF0000;">PCR qbeta and clone back into 3097</span> | <br/> | Nde1-HindIII | C-His tag | <br/> |- | 812<br/> | <br/> | pET30b-phoA<br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> |- | 938 | <br/> | PNGase F, | 1145 | <s>pET30b</s> pT7A30 | Nde1-Xho1 | pJet1.2, sequencing is good, need to subclone into pET30b | <br/> | <br/> | <br/> | C-His<br/> |- | 939 pET30b-Tte-ExoIII | <br/> | Tte-Exo III | 780 | <s>pET30b</s> pT7A30 | Nde1-Xho1<br/> | pJet1.2 sequencing is good, need to subclone into pET30b | <br/> | <br/> | <br/> | C-His |- | 942 pET28b-pMBP-VVN | <br/> | VVN endonuclease | 716 | pET28b-pMBP<br/> | Nco1-Xho1 | already in pJet1.2 <span style="color:#FF0000;">need to check by digestion</span> | <br/> | <br/> | <br/> | C-His<br/> |- | 3122 | <br/> | Crm197, secreted | 1743 | pET30b<br/> | Nde1-Xho1<br/> | already in pJet1.2<br/> | <br/> | <br/> | <br/> | C-His |- | 931 | <br/> | PAK16D ligase | 2267 | pET28b | Nde1-HindIII-Xho1<br/> | pJet1.2 fragment 2 is good fragment 1 is also good <span style="color:#FF0000;">need to do digestions</span> <span style="color:#FF0000;">seqeucning result: junk, E coli chromesome DNA</span> | <br/> | <br/> | <br/> | N-His |- | pT7A30-pMBP <span style="color:#FF0000;">945 </span><br/> | <br/> | <span style="color:#FF0000;">pT7A30-pMBP</span><br/> | <br/> | pT7A30 | Xba1-Nco1<br/> | PCR from 1281 pMAL-p5E or 1282 pMAL-p5E-AvPAL <span style="color:#FF0000;">sequencing is good, completed</span> | <br/> | <br/> | <br/> | secreted to periplasm |- | pT7A30-6His-pMBP <span style="color:#FF0000;">947 </span><br/> | <br/> | <span style="color:#FF0000;">pT7A30-6His-pMBP</span><br/> | <br/> | pT7A30<br/> | <span style="color: rgb(0, 0, 0); font-family: Arial, Verdana, sans-serif; font-size: 12px; background-color: rgb(251, 251, 239);">Xba1-Nco1</span><br/> | PCR from 1281 pMAL-p5E or 1282 pMAL-p5E-AvPAL (as template) <span style="color:#FF0000;">sequencing is good, completed</span> | <br/> | <br/> | <br/> | N-6Xhis tag MBP secreted to periplasm<br/> |- | 944 | <br/> | pET30b-pfu-exo-<br/> | <br/> | <br/> | <br/> | 944 pET30b-pfu-exo digestion <span style="color:#FF0000;">, sequencing result: not the right clone</span> | <br/> | <br/> | <br/> | <br/> |- | <span style="color:#FF0000;">824</span><br/> | 0 | <span style="color:#FF0000;">pET28b-VVTopo</span><br/> | 969<br/> | pET28b | Nde1 + Xho1<br/> | subclone from 832 pMiniT-VVTopo using Nde1 + Xho1 n<span style="color:#FF0000;">eed to do PCR to confirm identity, if it is not the right clone, use 945 or 947 as vector</span> <s>824pCold-GST-VVTopoin pCold-GST vector, digestion , 824pT7A3-VVTpopin pT7A3 vector digestion</s> in pET28 vector, sequenced | <br/> | <br/> | <br/> | Customer's order #: 4988 |- | <br/> | <br/> | <span style="color:#FF0000;">3107 pFastbac-Cel1</span><br/> | <br/> | <br/> | <br/> | GGATCC<br/>ACCATGAAGTTCTTGGTGAACGTGGCGCTGGTGTTCATGGTCGTGTACATCTCCTACATTT ACGCGTGGGGCAAAGAAGGCCACTACGCCGTGTGTAAGATTGCGGAGGGCTTTTTGTCGGA AGACGCATTGGGAGCGGTCAAAGCCTTGTTGCCGGACTACGCGGAAGGCGACTTGGCAGCC GTATGTAGCTGGGCCGACGAGATCAGACACAACTTTCACTGGAGATGGTCGGGCCCACTGC ATTACGTCGACACGCCGGATTACAGATGCAACTACGAGTACTGCCGCGACTGTCACGACTT CAGAGGCCACAAAGACATTTGCGTCACGGGCGCGATATACAACTACACGAAACAATTGACG TCGGGCTACCACAACAGTGGCTCCGAGATTCGATACAACCTCACGGAGGCCTTGATGTTCC TCTCGCATTTCATTGGCGACGTGCACCAACCGCTGCATGTGGGCTTTACGGGCGATGAAGG CGGAAATACGATCATTGTCCGTTGGTACCGCAGAAAGACCAACCTCCACCACATATGGGAC AACATGATCATCGACTCGGCGTTGAAGACCTACTACAACAGCGACCTGGCCATAATGATCC AGGCGATTCAAAGAAACATCACCGGCGATTGGTCCTTTGACATCAGCAGCTGGAAGAACTG TGCCAGTGACGACACTGCTTGTCCGAACCTATACGCGTCGGAGAGCATCTCGTTGGCCTGT AAATTTGCCTACAGAAATGCCACCCCCGGTTCGACGCTGGGCGACGACTACTTCTTGTCGC GATTGCCGATTGTTGAAAAACGCCTCGCCCAAGGCGGTATTAGATTGGCCGCCACCTTGAA CCGTATTTTTGCCTCGCAACCGAAAATCTCGC GAAACACGAAGACAAGAGAG CGAGAAG ACGACGCCGGTAGACTACATCGAGTGGTCGCCAT TGCAACAGT TCAGCGGAAGCCACCACC ATCACCACCATCATCACGGC<br/>ctcgag CAT CAT CAT CAT CAT CAC<br/>TGATAA AAGCTT | <br/> | <br/> | <br/> | <br/> |- | 995<br/> | <br/> | pCold-GST-CD133<br/> | <br/> | 3161<br/> | <br/> | no good | <br/> | <br/> | <br/> | <br/> |- | 994<br/> | <br/> | pMiniT2-LAMP-1_CD133<br/> | <br/> | <br/> | <br/> | no good | <br/> | <br/> | <br/> | <br/> |- | 993pCold-GST-M13-Gene8993<br/> | <br/> | pCold-GST-M13-Gene8<br/> | <br/> | 3161<br/> | <br/> | +993pCold-GST-M13-Gene8digestion | <br/> | <br/> | <br/> | <br/> |- | 992pCold-GST-V5992<br/> | <br/> | pCold-GST-V5<br/> | <br/> | 3161<br/> | <br/> | +992pCold-GST-V5digestion | <br/> | <br/> | <br/> | <br/> |- | 991pCold-GST-Flag991<br/> | <br/> | pCold-GST-Flag<br/> | <br/> | 3161<br/> | <br/> | +991pCold-GST-Flagdigestion | <br/> | <br/> | <br/> | <br/> |- | 990pCold-GST-HA990<br/> | <br/> | pCold-GST-HA<br/> | <br/> | 3161<br/> | <br/> | +990pCold-GST-HAdigestion | <br/> | <br/> | <br/> | <br/> |- | 989pCold-GST-Myc989<br/> | <br/> | pCold-GST-Myc<br/> | <br/> | 3161<br/> | <br/> | +989pCold-GST-Mycdigestion | <br/> | <br/> | <br/> | <br/> |- | 988<br/> | <br/> | 987 pCMVS-LAMP-1_CD133<br/> | <br/> | 192<br/> | <br/> | - | <br/> | <br/> | <br/> | <br/> |- | 985pCold-GST-3C-AP205-M13-g3p985<br/> | <br/> | pCold-GST-3C-AP205-M13-g3p<br/> | <br/> | 2047<br/> | <br/> | + 985pCold-GST-3C-AP205-M13-g3pdigestion | <br/> | <br/> | <br/> | <br/> |- | 984<br/> | <br/> | pT7A3-6H-SBP-SUMO-3C<br/> | <br/> | 1279 pT7A3<br/> | <br/> | not good, sequencing is junk | <br/> | <br/> | <br/> | <br/> |- | 997 | <br/> | pT7A3-Qbeta<br/> | <br/> | 1279 pT7A3<br/> | <br/> | 997pT7A3-QbetaDigestion , sequenced , good, <span style="color:#008000;">completed</span> | <br/> | <br/> | <br/> | <br/> |- | 986 | <br/> | pT7A3-6His-NLS-Cas9-NLS-6xHis<br/> | <br/> | 1708 pT7A3-HTNCre<br/> | <br/> | 986pT7A3-6His-NLS-Cas9-NLS-6xHis digestion looks good, but need to do sequencing | <br/> | <br/> | <br/> | <br/> |- | 998<br/> | <br/> | pT7A3-6His-NLS-dCas9-NLS-6xHis<br/> | <br/> | 1708 pT7A3-HTNCre<br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> |- | 999<br/> | <br/> | pT7A30-SssI<br/> | <br/> | 1916<br/> | <br/> | based on 1916 gene synthesis | <br/> | <br/> | <br/> | <br/> |- | 1000 | <br/> | pT7A3-6His-NLS-FnCpf1-NLS-6xHis<br/> | <br/> | 1708 pT7A3-HTNCre<br/> | <br/> | no good | <br/> | <br/> | <br/> | <br/> |- | 3210<br/> | <br/> | pT7A30-Sumo-Ov-ASP1-BCMA<br/> | <br/> | 1727 pT7A30<br/> | EcoR1+ Nde1 | <br/> | <br/> | <br/> | <br/> | <br/> |- | 3218<br/> | <br/> | pT7A30-ULP1<br/> | <br/> | 1727 pT7A30<br/> | <br/> | PRC from 2209 ZM552-ULP1 | <br/> | <br/> | <br/> | <br/> |- | 3191<br/> | <br/> | <span style="color: rgb(0, 0, 0); font-family: Arial, Verdana, sans-serif; font-size: 12px; white-space: nowrap;">pT7A3-GST-Avitag</span><br/> | <br/> | <br/> | <br/> | sequencing: empty vector | <br/> | <br/> | <br/> | <br/> |- | 3192 | <br/> | pCMV5-PD-1-Avi-6H-BirA<br/> | <br/> | <br/> | <br/> | <span style="color:#008000;">sequencing good, completed</span> | <br/> | <br/> | <br/> | <br/> |- | 3166 <br/> | <br/> | pCMV-mIgG2b-HC<br/> | <br/> | <br/> | <br/> | <span style="color: rgb(0, 128, 0);">sequencing good, completed</span><br/> | <br/> | <br/> | <br/> | <br/> |- | 3197 | <br/> | pT7A3-GST-BCMA<br/> | <br/> | <br/> | <br/> | no good 6/27/2018: sequencing: empty vector | <br/> | <br/> | <br/> | <br/> |- | 3194 <br/> | <br/> | pCMV5-IL2-BCMA-(3c)-Fc-WPRE-OriP<br/> | <br/> | <br/> | <br/> | no good | <br/> | <br/> | <br/> | <br/> |- | 2503 <br/> | <br/> | pCold-VVTopo<br/> | <br/> | <br/> | <br/> | subcloning Nde1 -Xho1, digestion: not good, sequencing: junk | <br/> | <br/> | <br/> | <br/> |- | 3218<br/> | <br/> | pT7A30-ULP1<br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> |- | 2509 | <br/> | pT7A30-pMBP-Cid<br/> | <br/> | 947 pT7A30-6His-pMBP<br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> |- | 3204<br/> | <br/> | pCMV5-OX40-Avi-6H-BirA<br/> | <br/> | 3192 pCMV5-PD-1-Avi-6H-BirA<br/> | <br/> | from 3203 pANT7_cGST-TNFRSF4<br/> | <br/> | <br/> | <br/> | <br/> |- | 3199<br/> | <br/> | pCMV5-BCMA-Avi-6H-BirA<br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> |- | 3196<br/> | <br/> | pCMV5-CD47-Avi-6H-BirA<br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> |- | 3195<br/> | <br/> | pEF1-HTLV-LAMP-1-BCMA<br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> |- | 3235<br/> | <br/> | pCMV5-IL2-MICA-(3c)-Fc-WPRE-OriP<br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> |- | 3234 <br/> | <br/> | pCMV5-IL2-PD-1-(3c)-Fc-WPRE-OriP<br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> |- | 3233 <br/> | <br/> | pCMV5-IL2-CD47-(3c)-Fc-WPRE-OriP<br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> |- | 3232 <br/> | <br/> | pCMV5-Avi-6H-BirA<br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> |- | 2199 <br/> | <br/> | pET30b-Ecoli-Muts<br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> |- | 2204 <br/> | <br/> | pET30b-Ecoli-Muts-D835R<br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> |- | 129<br/> | <br/> | pL42-TthMuts<br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> |} [[Category:To Do]] [[Category:Tasks]]
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