Cloning Tasks
Urgent Tasks
| ID | Clone Name | Product Name | Note | Order ID | Cat # |
| 162 |
pCold-MBP-(Tev)-GST-(3C)-Tat-NLS-iFLP-6H |
Flp | 5148 |
EG-1002 | |
| 2108 | pCold-Tat-NLS-iFLP-6H |
Flp | 5148 |
EG-1002 | |
| 2503 |
pCold-GST-VVTopo |
Vaccinia Virus Topoisomerase I |
5161 5154
|
EG-167 | |
|
2291
|
pT7A3-SortaseAM-N6H |
Bifunctional Protein Ligase/Protease Sortase A |
expressed | 5159 |
EG-42 |
| 2232 |
pT7A3-SortaseAM-C6H |
Bifunctional Protein Ligase/Protease Sortase A |
no expression | 5159 |
EG-42 |
| ID | Status | Name | DNA Size | Vector | Method | Progress |
Date | Enzyme |
Expression | Note |
| 3127 |
pET28b-qBeta-qBeta-6XHis |
PCR qbeta and clone back into 3097 | Nde1-HindIII | C-His tag | ||||||
| 812 |
pET30b-phoA |
|||||||||
| 938 | PNGase F, | 1145 |
pT7A30 |
Nde1-Xho1 | pJet1.2, sequencing is good, need to subclone into pET30b | C-His | ||||
| 939 pET30b-Tte-ExoIII | Tte-Exo III | 780 |
pT7A30 |
Nde1-Xho1 |
pJet1.2 sequencing is good, need to subclone into pET30b |
C-His | ||||
| 942 pET28b-pMBP-VVN | VVN endonuclease | 716 | pET28b-pMBP |
Nco1-Xho1 |
already in pJet1.2 need to check by digestion
|
C-His | ||||
| 3122 | Crm197, secreted | 1743 | pET30b |
Nde1-Xho1 |
already in pJet1.2 |
C-His | ||||
| 931 | PAK16D ligase | 2267 | pET28b | Nde1-HindIII-Xho1 |
pJet1.2 fragment 2 is good fragment 1 is also good need to do digestions seqeucning result: junk, E coli chromesome DNA |
N-His | ||||
| pT7A30-pMBP 945 |
pT7A30-pMBP |
pT7A30 | Xba1-Nco1 |
PCR from 1281 pMAL-p5E or 1282 pMAL-p5E-AvPAL sequencing is good, completed |
secreted to periplasm | |||||
| pT7A30-6His-pMBP 947 |
pT7A30-6His-pMBP |
pT7A30 |
Xba1-Nco1 |
PCR from 1281 pMAL-p5E or 1282 pMAL-p5E-AvPAL (as template) sequencing is good, completed |
N-6Xhis tag MBP secreted to periplasm | |||||
| 944 | pET30b-pfu-exo- |
944 pET30b-pfu-exo digestion , sequencing result: not the right clone | ||||||||
| 824 |
0 | pET28b-VVTopo |
969 |
pET28b | Nde1 + Xho1 |
subclone from 832 pMiniT-VVTopo using Nde1 + Xho1 need to do PCR to confirm identity, if it is not the right clone, use 945 or 947 as vector
in pET28 vector, sequenced |
Customer's order #: 4988 | |||
| 3107 pFastbac-Cel1 |
GGATCC AAGCTT |
|||||||||
| 995 |
pCold-GST-CD133 |
3161 |
no good | |||||||
| 994 |
pMiniT2-LAMP-1_CD133 |
no good | ||||||||
| 993pCold-GST-M13-Gene8993 |
pCold-GST-M13-Gene8 |
3161 |
+993pCold-GST-M13-Gene8digestion | |||||||
| 992pCold-GST-V5992 |
pCold-GST-V5 |
3161 |
+992pCold-GST-V5digestion | |||||||
| 991pCold-GST-Flag991 |
pCold-GST-Flag |
3161 |
+991pCold-GST-Flagdigestion | |||||||
| 990pCold-GST-HA990 |
pCold-GST-HA |
3161 |
+990pCold-GST-HAdigestion | |||||||
| 989pCold-GST-Myc989 |
pCold-GST-Myc |
3161 |
+989pCold-GST-Mycdigestion | |||||||
| 988 |
987 pCMVS-LAMP-1_CD133 |
192 |
- | |||||||
| 985pCold-GST-3C-AP205-M13-g3p985 |
pCold-GST-3C-AP205-M13-g3p |
2047 |
+ 985pCold-GST-3C-AP205-M13-g3pdigestion | |||||||
| 984 |
pT7A3-6H-SBP-SUMO-3C |
1279 pT7A3 |
not good, sequencing is junk | |||||||
| 997 | pT7A3-Qbeta |
1279 pT7A3 |
997pT7A3-QbetaDigestion , sequenced , good, completed | |||||||
| 986 | pT7A3-6His-NLS-Cas9-NLS-6xHis |
1708 pT7A3-HTNCre |
986pT7A3-6His-NLS-Cas9-NLS-6xHis digestion looks good, but need to do sequencing | |||||||
| 998 |
pT7A3-6His-NLS-dCas9-NLS-6xHis |
1708 pT7A3-HTNCre |
||||||||
| 999 |
pT7A30-SssI |
1916 |
based on 1916 gene synthesis | |||||||
| 1000 | pT7A3-6His-NLS-FnCpf1-NLS-6xHis |
1708 pT7A3-HTNCre |
no good | |||||||
| 3210 |
pT7A30-Sumo-Ov-ASP1-BCMA |
1727 pT7A30 |
EcoR1+ Nde1 | |||||||
| 3218 |
pT7A30-ULP1 |
1727 pT7A30 |
PRC from 2209 ZM552-ULP1 | |||||||
| 3191 |
pT7A3-GST-Avitag |
sequencing: empty vector | ||||||||
| 3192 | pCMV5-PD-1-Avi-6H-BirA |
sequencing good, completed | ||||||||
| 3166 |
pCMV-mIgG2b-HC |
sequencing good, completed |
||||||||
| 3197 | pT7A3-GST-BCMA |
no good 6/27/2018: sequencing: empty vector | ||||||||
| 3194 |
pCMV5-IL2-BCMA-(3c)-Fc-WPRE-OriP |
no good | ||||||||
| 2503 |
pCold-VVTopo |
subcloning Nde1 -Xho1, digestion: not good, sequencing: junk | ||||||||
| 3218 |
pT7A30-ULP1 |
|||||||||
| 2509 | pT7A30-pMBP-Cid |
947 pT7A30-6His-pMBP |
||||||||
| 3204 |
pCMV5-OX40-Avi-6H-BirA |
3192 pCMV5-PD-1-Avi-6H-BirA |
from 3203 pANT7_cGST-TNFRSF4 |
|||||||
| 3199 |
pCMV5-BCMA-Avi-6H-BirA |
|||||||||
| 3196 |
pCMV5-CD47-Avi-6H-BirA |
|||||||||
| 3195 |
pEF1-HTLV-LAMP-1-BCMA |
|||||||||
| 3235 |
pCMV5-IL2-MICA-(3c)-Fc-WPRE-OriP |
|||||||||
| 3234 |
pCMV5-IL2-PD-1-(3c)-Fc-WPRE-OriP |
|||||||||
| 3233 |
pCMV5-IL2-CD47-(3c)-Fc-WPRE-OriP |
|||||||||
| 3232 |
pCMV5-Avi-6H-BirA |
|||||||||
| 2199 |
pET30b-Ecoli-Muts |
|||||||||
| 2204 |
pET30b-Ecoli-Muts-D835R |
|||||||||
| 129 |
pL42-TthMuts |