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= Need to sequence = '''=====finished but have not sequenced yet======''' {| border="1" cellspacing="1" cellpadding="1" style="width: 815px;" |- | # | Name | Status | Date | picture | Vector# | style="width: 79px;" | Enzymes | style="width: 58px;" | Size | style="width: 77px;" | Antibiotics | style="width: 41px;" | Method | Note | Next Action | style="width: 147px;" | QC_sequence clone# |- | 2509 | pT7A30-pMBP-Cid<br/> | <br/> | <br/> | [http://www.excellgen.net/eln/index.php/2509_pT7A30-pMBP-Cid result] | <br/> | style="width: 79px;" | Nde1 -Xho1<br/> | style="width: 58px;" | 978 | style="width: 77px;" | K | style="width: 41px;" | Subclone | compared with + | re-doing | style="width: 147px;" | <br/> |- | 3204 | <br/> | <br/> | <br/> | <br/> | <br/> | style="width: 79px;" | <br/> | style="width: 58px;" | <br/> | style="width: 77px;" | <br/> | style="width: 41px;" | <br/> | <br/> | done | style="width: 147px;" | <br/> |- | 3210 | pT7A30-Sumo-Ov-ASP1-BCMA<br/> | <br/> | <br/> | [http://www.excellgen.net/eln/index.php/3210_pT7A30-Sumo-Ov-ASP1-BCMA result] | 1801 | style="width: 79px;" | Nde1-EcoR1<br/> | style="width: 58px;" | 999 | style="width: 77px;" | K | style="width: 41px;" | Subclone | compared with + | sequencing T7 Term;done<br/> | style="width: 147px;" | <br/> |- | 3215 | pCMV5-MICA-a-Avi-6H-BirA<br/> | <br/> | <br/> | [http://www.excellgen.net/eln/index.php/3215_pCMV5-MICA-a-Avi-6H-BirA#Cloning result] | 3237 | style="width: 79px;" | <br/> | style="width: 58px;" | <br/> | style="width: 77px;" | Amp | style="width: 41px;" | subclone | compared with +and 3237 Vector digestion<br/> | done | style="width: 147px;" | <br/> |- | 3199 | pT7A3-GST-Avitag<br/> | <br/> | <br/> | [http://www.excellgen.net/eln/index.php/3199_pCMV5-BCMA-Avi-6H-BirA result] | 3237 | style="width: 79px;" | <br/> | style="width: 58px;" | <br/> | style="width: 77px;" | Amp | style="width: 41px;" | subclone | compared with +and 3237 Vector digestion<br/> | done | style="width: 147px;" | <br/> |- | 3196 | pCMV5-CD47-Avi-6H-BirA<br/> | <br/> | <br/> | [http://www.excellgen.net/eln/index.php/3196_pCMV5-CD47-Avi-6H-BirA result] | 3237 | style="width: 79px;" | <br/> | style="width: 58px;" | <br/> | style="width: 77px;" | Amp | style="width: 41px;" | subclone | compared with +and 3237 Vector digestion<br/> | done | style="width: 147px;" | <br/> |- | 941 | pT7A3-pMBP-T7-Endo-1<br/> | <br/> | <br/> | [http://www.excellgen.net/eln/index.php/941_pT7A3-pMBP-T7-Endo-1 result] | 947 | style="width: 79px;" | <br/> | style="width: 58px;" | <br/> | style="width: 77px;" | K | style="width: 41px;" | subclone | comnpare with + | done | style="width: 147px;" | <br/> |- | 129 | <br/> | <br/> | <br/> | <br/> | <br/> | style="width: 79px;" | <br/> | style="width: 58px;" | <br/> | style="width: 77px;" | <br/> | style="width: 41px;" | <br/> | <br/> | <br/> | style="width: 147px;" | <br/> |- | 994 | <br/> | <br/> | <br/> | <br/> | <br/> | style="width: 79px;" | <br/> | style="width: 58px;" | <br/> | style="width: 77px;" | <br/> | style="width: 41px;" | <br/> | <br/> | <br/> | style="width: 147px;" | <br/> |- | 3197 | <br/> | <br/> | <br/> | <br/> | <br/> | style="width: 79px;" | <br/> | style="width: 58px;" | <br/> | style="width: 77px;" | <br/> | style="width: 41px;" | <br/> | <br/> | <br/> | style="width: 147px;" | <br/> |- | 995 | <br/> | <br/> | <br/> | <br/> | <br/> | style="width: 79px;" | <br/> | style="width: 58px;" | <br/> | style="width: 77px;" | <br/> | style="width: 41px;" | <br/> | <br/> | <br/> | style="width: 147px;" | <br/> |} = Completed and sequenced = '''==========completed and sequenced==============''' {| border="1" cellspacing="1" cellpadding="1" style="width:500px;" |- | '''ID''' | '''Status''' | '''Date''' | '''Name''' | '''Progress'''<br/> | '''Date''' | Supervisor's Note | Clone # | Status |- | <span style="color:#FF0000;">3127</span><br/> | <br/> | <br/> | <span style="color:#FF0000;">pET28b-qBeta-qBeta-6XHis</span><br/> | <span style="color:#FF0000;">PCR qbeta and clone back into 3097</span> | already finished on 5/3/2018,sequenced | glycerol stock ? expression screening? | <br/> | GS made |- | 938 | <br/> | <br/> | PNGase F, | pJet1.2, sequencing is good, need to subclone into pET30b | finished | glycerol stock ? expression screening?<br/> | <br/> | GS made |- | 939 pET30b-Tte-ExoIII | <br/> | <br/> | Tte-Exo III | pJet1.2 sequencing is good, need to subclone into pET30b | finished | glycerol stock ? expression screening?<br/> | <br/> | GS made |- | 942 pET28b-pMBP-VV | <br/> | <br/> | VVN endonuclease | already in pJet1.2 <span style="color:#FF0000;">need to check by digestion</span> | finished | glycerol stock ? expression screening?<br/> | <br/> | GS made |- | 3122 | <br/> | <br/> | Crm197, secreted | already in pJet1.2,subclone to pET30b(vector#500), <span style="background-color:#FF0000;">alrady finished</span> | [http://www.excellgen.net/eln/index.php/3122_pET30b-CRM197 done],finished | glycerol stock ? expression screening? | <br/> | GS made |- | <span style="color:#FF0000;">945 </span><br/> | <br/> | <br/> | <span style="color:#FF0000;">pT7A30-pMBP</span><br/> | PCR from 1281 pMAL-p5E or 1282 pMAL-p5E-AvPAL <span style="color:#FF0000;">sequencing is good, completed</span> | <br/> | sequencing verfied | <br/> | GS made |- | <span style="color:#FF0000;">947 </span><br/> | <br/> | <br/> | <span style="color:#FF0000;">pT7A30-6His-pMBP</span><br/> | PCR from 1281 pMAL-p5E or 1282 pMAL-p5E-AvPAL (as template) <span style="color:#FF0000;">sequencing is good, completed</span> | <br/> | sequencing verfied<br/> | <br/> | GS made |- | 944 | <br/> | <br/> | pET30b-pfu-exo-<br/> | 944 pET30b-pfu-exo digestion <span style="color:#FF0000;">, sequencing result: finished</span> | <br/> | glycerol stock ? expression screening?<br/> | <br/> | GS made |- | <span style="color:#FF0000;">824</span><br/> | 0 | <br/> | <span style="color:#FF0000;">pET28b-VVTopo</span><br/> | subclone from 832 pMiniT-VVTopo using Nde1 + Xho1 in pET28 vector, sequenced | <br/> | glycerol stock ? expression screening?<br/> | <br/> | GS made |- | 993<br/> | <br/> | <br/> | pCold-GST-M13-Gene8<br/> | +993pCold-GST-M13-Gene8digestion | <br/> | sequencing with T7 Term, expression screening?<br/> | <br/> | <br/> |- | 992<br/> | <br/> | <br/> | pCold-GST-V5<br/> | +992pCold-GST-V5digestion | <br/> | sequencing with T7 Term, then expression screening | <br/> | <br/> |- | 991 <br/> | <br/> | <br/> | pCold-GST-Flag<br/> | +991pCold-GST-Flagdigestion | <br/> | sequencing with T7 Term, then expression screening<br/> | <br/> | <br/> |- | 990<br/> | <br/> | <br/> | pCold-GST-HA<br/> | +990pCold-GST-HAdigestion | <br/> | sequencing with T7 Term, then expression screening<br/> | <br/> | <br/> |- | 989 <br/> | <br/> | <br/> | pCold-GST-Myc<br/> | +989pCold-GST-Mycdigestion | <br/> | sequencing with T7 Term, then expression screening<br/> | <br/> | <br/> |- | 985 <br/> | <br/> | <br/> | pCold-GST-3C-AP205-M13-g3p<br/> | 985pCold-GST-3C-AP205-M13-g3pdigestion, good sequenced, | <br/> | expression screening<br/> | <br/> | <br/> |- | 997 | <br/> | <br/> | pT7A3-Qbeta<br/> | 997pT7A3-QbetaDigestion , sequenced , good, <span style="color:#008000;">completed</span> | <br/> | no his tag, do not need to do expression screening | <br/> | <br/> |- | 986 | <br/> | <br/> | pT7A3-6His-NLS-Cas9-NLS-6xHis<br/> | 986pT7A3-6His-NLS-Cas9-NLS-6xHis digestion looks good, <span style="color:#FF0000;">good, sequenced</span> | <br/> | expression screening?<br/> | <br/> | GS made |- | <div>984</div><div><br/></div><div><br/></div><div><br/></div> | | <br/> | pT7A3-6H-SBP-SUMO-3C | 6/6/18 sequencing verified 6/5/2018 sequenced<br/> | <br/> | expression screening?<br/> | <br/> | GS made |- | 3192 | <br/> | <br/> | pCMV5-PD-1-Avi-6H-BirA<br/> | <span style="color:#008000;">sequencing good, completed</span> | <br/> | make miniprep from 15 ml | <br/> | GS made |- | 3166 <br/> | <br/> | <br/> | pCMV-mIgG2b-HC<br/> | <span style="color: rgb(0, 128, 0);">sequencing good, completed</span><br/> | <br/> | make miniprep from 15 ml<br/> | <br/> | GS made |- | | | | | <br/> | <br/> | <br/> | <br/> | <br/> |- | <br/> | | <br/> | | | <br/> | <br/> | <br/> | <br/> |- | <br/> | | <br/> | | | <br/> | <br/> | <br/> | <br/> |- | | | <br/> | | <br/> | <br/> | <br/> | <br/> | <br/> |- | | | <br/> | | <br/> | <br/> | <br/> | <br/> | <br/> |- | <br/> | | <br/> | | | <br/> | <br/> | <br/> | <br/> |- | | | <br/> | | | | <br/> | <br/> | <br/> |} {| border="1" cellspacing="1" cellpadding="1" style="width: 759px;" |- | # | Name | Status | Date | style="width: 37px;" | picture | style="width: 91px;" | Vector# | Enzymes | Size | Antibiotics | Method | Note | Next Action | QC_sequence clone# | Final Status |- | 3233 | pCMV5-IL2-CD47-(3c)-Fc-WPRE-OriP<br/> | <br/> | <br/> | style="width: 37px;" | [http://www.excellgen.net/eln/index.php/3233_pCMV5-IL2-CD47-(3c)-Fc-WPRE-OriP result] | style="width: 91px;" | 1393 | Nhe1-KpnI<br/> | 375 | Amp | Subclone | compared with + | sequencing good, completed<br/> | #1 | GS made |- | 2503 | pCold-GST-VVTopo<br/> | <br/> | <br/> | style="width: 37px;" | [http://www.excellgen.net/eln/index.php/2503_pCold-GST-VVTopo result] | style="width: 91px;" | 3161 | Nde1 -Xho1<br/> | 951 | K | Subclone | compared with + | sequencing good, completed<br/> | #4 | GS made |- | 3194 | pCMV5-IL2-BCMA-(3c)-Fc-WPRE-OriP<br/> | <br/> | <br/> | style="width: 37px;" | [http://www.excellgen.net/eln/index.php/3194_pCMV5-IL2-BCMA-(3c)-Fc-WPRE-OriP result] | style="width: 91px;" | 1393 | Nhe1-KpnI<br/> | 174bp | Amp | PCR | <br/> | sequencing good, completed<br/> | #2 | GS made |- | 3215 | <span style="font-family: Arial, Verdana, sans-serif; font-size: 12px; background-color: rgb(251, 251, 239);">pCMV5-MICA-a-Avi-6H-BirA</span><br/> | <br/> | <br/> | style="width: 37px;" | [http://www.excellgen.net/eln/index.php/3204_pCMV5-OX40-Avi-6H-BirA#Results result] | style="width: 91px;" | 3192 | Nhe1-Kpn1 | 285bp | Amp | PCR | <br/> | sequencing insert is good, subclone to 3232<br/> | #1 | sub |- | 3204 | <span style="font-family: Arial, Verdana, sans-serif; font-size: 12px; background-color: rgb(251, 251, 239);">pCMV5-OX40-Avi-6H-BirA</span><br/> | <br/> | <br/> | style="width: 37px;" | [http://www.excellgen.net/eln/index.php/3215_pCMV5-MICA-a-Avi-6H-BirA#Cloning result] | style="width: 91px;" | 1393 | Nhe1+kpn1 | 564bp | Amp | PCR | <br/> | sequencing insert is good, subclone to 3232 | #5 | sub |- | 3191 | pT7A3-GST-Avitag<br/> | <br/> | <br/> | style="width: 37px;" | <br/> | style="width: 91px;" | 1851 | <br/> | <br/> | K | Annealing | <br/> | sequencing good, completed<br/> | <br/> | GS Made |- | 3232 | pCMV5-Avi-6H-BirA<br/> | <br/> | <br/> | style="width: 37px;" | <br/> | style="width: 91px;" | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> | <br/> |} [[Category:To Do]]
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