Completed Clones

Need to sequence 

=====finished but have not sequenced yet======

#

Name

Status

Date

picture

Vector#

Enzymes

Size

Antibiotics

Method

Note

Next Action

QC_sequence clone#

2509

pT7A30-pMBP-Cid

result

Nde1 -Xho1

978

K

Subclone

compared with +

re-doing

3204

done

3210

pT7A30-Sumo-Ov-ASP1-BCMA

result

1801

Nde1-EcoR1

999

K

Subclone

compared with +

sequencing T7 Term;done

3215

pCMV5-MICA-a-Avi-6H-BirA

result

3237

Amp

subclone

compared with +and 3237 Vector digestion

done

3199

pT7A3-GST-Avitag

result

3237

Amp

subclone

compared with +and 3237 Vector digestion

done

3196

pCMV5-CD47-Avi-6H-BirA

result

3237

Amp

subclone

compared with +and 3237 Vector digestion

done

941

pT7A3-pMBP-T7-Endo-1

result

947

K

subclone

comnpare with +

done

129

994

3197

995

Completed and sequenced 

==========completed and sequenced==============

ID	Status	Date	Name	Progress	Date	Supervisor's Note	Clone #	Status
3127			pET28b-qBeta-qBeta-6XHis	PCR qbeta and clone back into 3097	already finished on 5/3/2018,sequenced	glycerol stock ? expression screening?		GS made
938			PNGase F,	pJet1.2, sequencing is good, need to subclone into pET30b	finished	glycerol stock ? expression screening?		GS made
939 pET30b-Tte-ExoIII			Tte-Exo III	pJet1.2 sequencing is good, need to subclone into pET30b	finished	glycerol stock ? expression screening?		GS made
942 pET28b-pMBP-VV			VVN endonuclease	already in pJet1.2 need to check by digestion	finished	glycerol stock ? expression screening?		GS made
3122			Crm197, secreted	already in pJet1.2,subclone to pET30b(vector#500), alrady finished	done,finished	glycerol stock ? expression screening?		GS made
945			pT7A30-pMBP	PCR from 1281 pMAL-p5E or 1282 pMAL-p5E-AvPAL sequencing is good, completed		sequencing verfied		GS  made
947			pT7A30-6His-pMBP	PCR from 1281 pMAL-p5E or 1282 pMAL-p5E-AvPAL (as template) sequencing is good, completed		sequencing verfied		GS made
944			pET30b-pfu-exo-	944 pET30b-pfu-exo digestion , sequencing result: finished		glycerol stock ? expression screening?		GS made
824	0		pET28b-VVTopo	subclone from 832 pMiniT-VVTopo using Nde1 + Xho1 in pET28 vector, sequenced		glycerol stock ? expression screening?		GS made
993			pCold-GST-M13-Gene8	+993pCold-GST-M13-Gene8digestion		sequencing with T7 Term,  expression screening?
992			pCold-GST-V5	+992pCold-GST-V5digestion		sequencing with T7 Term, then expression screening
991			pCold-GST-Flag	+991pCold-GST-Flagdigestion		sequencing with T7 Term, then expression screening
990			pCold-GST-HA	+990pCold-GST-HAdigestion		sequencing with T7 Term, then expression screening
989			pCold-GST-Myc	+989pCold-GST-Mycdigestion		sequencing with T7 Term, then expression screening
985			pCold-GST-3C-AP205-M13-g3p	985pCold-GST-3C-AP205-M13-g3pdigestion, good sequenced,		expression screening
997			pT7A3-Qbeta	997pT7A3-QbetaDigestion , sequenced , good, completed		no his tag, do not need to do expression screening
986			pT7A3-6His-NLS-Cas9-NLS-6xHis	986pT7A3-6His-NLS-Cas9-NLS-6xHis digestion looks good, good, sequenced		expression screening?		GS made
984			pT7A3-6H-SBP-SUMO-3C	6/6/18 sequencing verified 6/5/2018 sequenced		expression screening?		GS made
3192			pCMV5-PD-1-Avi-6H-BirA	sequencing good, completed		make miniprep from 15 ml		GS made
3166			pCMV-mIgG2b-HC	sequencing good, completed		make miniprep from 15 ml		GS made

#

Name

Status

Date

picture

Vector#

Enzymes

Size

Antibiotics

Method

Note

Next Action

QC_sequence clone#

Final Status

3233

pCMV5-IL2-CD47-(3c)-Fc-WPRE-OriP

result

1393

Nhe1-KpnI

375

Amp

Subclone

compared with +

sequencing good, completed

#1

GS made

2503

pCold-GST-VVTopo

result

3161

Nde1 -Xho1

951

K

Subclone

compared with +

sequencing good, completed

#4

GS made

3194

pCMV5-IL2-BCMA-(3c)-Fc-WPRE-OriP

result

1393

Nhe1-KpnI

174bp

Amp

PCR

sequencing good, completed

#2

GS made

3215

pCMV5-MICA-a-Avi-6H-BirA

result

3192

Nhe1-Kpn1

285bp

Amp

PCR

sequencing insert is good, subclone to 3232

#1

sub

3204

pCMV5-OX40-Avi-6H-BirA

result

1393

Nhe1+kpn1

564bp

Amp

PCR

sequencing insert is good, subclone to 3232

#5

sub

3191

pT7A3-GST-Avitag

1851

K

Annealing

sequencing good, completed

GS Made

3232

pCMV5-Avi-6H-BirA