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Human Antibody Cloning
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== Human Lambda Vaiable == <pre>L3 VTSYVLTQPPSVSVAPGQTARITCGGNNI---GSKSVHWYQQKPGQAPVLVVYDDSDRPS 57 L1 --QSALTQPRSVSGSPGQSVTISCTGTSSDVGGYNYVSWYQQHPGKAPKLMIYDVSKRPS 58 L2 --QSVLTQPPSVSAAPGQKVTISCSGSSSNIG-NNYVSWYQQLPGTAPKLLIYDNNKRPS 57 . .**** *** :***.. *:* *.. : * **** ** ** *::** ..*** L3 GIPERFSGSNSGNTATLTISRVEAGDEADYYCQVWDSSSDHPT 100 L1 GVPDRFSGSKSGNTASLTISGLQAEDEADYYCCSYAGSYTFH- 100 L2 GIPDRFSGSKSGTSATLGITGLQTGDEADYYCGTWDSSLSA-- 98 *:*:*****:**.:*:* *: ::: ******* : .* </pre> == Human Kappa Variable == <pre>K1 DIQMTQSPSSLSASVGDRVTITCRASQDIT-NYVNWFQQRPGQAPKVLIYGASILETGVP 59 K4 DIQMTQSPSSLSASVGDRVTITCRASQSIS-SYLNWYQQKPGKAPKLLIYAASSLQSGVP 59 K2 --ELTQAPGTLSLSPGERATFSCRSSHSIRSRRVRWYQHKPGQAPRLVIHGVSNRASGIS 58 K3 EIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIP 60 :**:*.:** * *:*.*::**:*:.: : *:*::**:**:::*:..* :*: K1 SRFSGSGSGTDFTFTISSLQPEDIATYYCQQYDTLPLTFGGGTKVDIKR 108 K4 SRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTP------------- 95 K2 DRFSGSGSGTDFTLTITRVEPEDFALYYCQVYGASSYTFGQGTKLERKR 107 K3 DRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSP------------- 96 .************:**: ::***:* **** : </pre> == Human Light Chain Kappa Constant == ACGGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAG '''TVAAPSVF'''IFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVT<span style="color:#FF0000;">KSFNRGEC</span> <pre> kappa --TVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQ 58 L1 GQPKANPTVTLFPPSSEELQANKATLVCLISDFYPG---AVTVAWKADGSPVKAGVETTK 57 L2 GQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG---AVTVAWKADSSPVKAGVETTT 57 * * * **** * * * *** *** * * * kappa DSKDS--TYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC 106 L1 PSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS- 106 L2 PSKSNNKYA-ASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS- 105 ** ** * * * * * *** * * </pre> == Human Light Chain Lambda 1 Constant == GGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAG<span style="color:#FF0000;">AAGACAGTGGCCCCTACAGAATGTTCA</span>TAG GQPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADGSPVKAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS <pre>L1 GQPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADGSPVKAGVETTKPSK 60 L2 GQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSK 60 ***** *:*************************************.**********.*** L1 QSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS 106 L2 -SNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS 105 ********************************************* L1 GGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAA 60 L2 GGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA 60 ************** **** * ******************************** *** L1 GCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTG 120 L2 GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTG 120 ***************** ******** ** ******************** ********* L1 GCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAA 180 L2 GCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAA 180 *************** ********************************* ********** L1 CAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAG 240 L2 CAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAG 240 ** ***************************** ************** ************ L1 TCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG 300 L2 TCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG 300 ************************************************************ L1 GCCCCTACAGAATGTTCATAG 321 L2 GCCCCTACAGAATGTTCATAG 321 ********************* </pre> == Human Light Chain Lambda 2 Constant == GGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAG<span style="color:#FF0000;">AAGACAGTGGCCCCTACAGAATGTTCA</span>TAG GQPKAAPS'''VTLFPPSS'''EELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKT<span style="color:#FF0000;">VAPTECS</span> == Human Heavy Chain G1 Constant == GCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGA A'''STKGPSV'''FPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPS<span style="color:#FF0000;">NTKVDK</span>KVEPKSCDKTHT CPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK to make Fab: ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPS<span style="color:#FF0000;">NTKVDK</span>KVEPKSCDKTHT GS linker aviTag GGSGSAG GLNDIFEAQKIEWHE- == Human Heavy Chain G2 Constant == GCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCTCTGACCAGCGGCGTGCACACCTTCCCAGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAACTTCGGCACCCAGACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGTCGAGTGCCCACCGTGCCCAGCACCACCTGTGGCAGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGA ASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSNFGTQTYTCNVDHKPSNTKVDKTVERKCCVECPPCPAPPVAGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTFRVVSVLTVVHQDWLNGKEYKCKVSNKGLPAPIEKTISKTKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPMLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK == Human Heavy Chain G3 Constant == GCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCAGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACACCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCTCAAAACCCCACTTGGTGACACAACTCACACATGCCCACGGTGCCCAGAGCCCAAATCTTGTGACACACCTCCCCCGTGCCCACGGTGCCCAGAGCCCAAATCTTGTGACACACCTCCCCCATGCCCACGGTGCCCAGAGCCCAAATCTTGTGACACACCTCCCCCGTGCCCAAGGTGCCCAGCACCTGAACTCCTGGGAGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGATACCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGACAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGGAGAGCAGCGGGCAGCCGGAGAACAACTACAACACCACGCCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACATCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCGCTTCACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAGTCCTAG A'''STKGPSV'''FPLAPCSRSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYTCNVNHKPSNTKVDKRVELKTPLGDTTHTCPRCPEPKSCDTPPPCPRCPEPKSCDTPPPCPRCPEPKSCDTPPPCPRCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFKWYVDGVEVHNAKTKPREEQYNSTFRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKTKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESSGQPENNYNTTPPMLDSDGSFFLYSKLTVDKSRWQQGNIFSCSVMHEALHNRFTQKSLSLSPGK == Human Heavy Chain G4 Constant == GCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCCGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACGAAGACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGTCCAAATATGGTCCCCCATGCCCATCATGCCCAGCACCTGAGTTCCTGGGGGGACCATCAGTCTTCCTGTTCCCCCCAAAACCCAAGGACACTCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGTGGATGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTTCAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAGCCACAGGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAGGCTCACCGTGGACAAGAGCAGGTGGCAGGAGGGGAATGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACACAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGA ASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYGPPCPSCPAPEFLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLSLSPGK == Human IgM Constant == G'''SASAPTL'''FPLVSCENSPSDTSSVAVGCLAQDFLPDSITFSWKYKNNSDISSTRGFPSVLRGGKYAATSQVLLPSKDVMQGTDEHVVCKVQHPNGN'''<span style="color:#FF0000;">KEKNVPLP</span>'''VIAELPPKVSVFVPPRDGFFGNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKESGPTTYKVTSTLTIKESDWLGQSMFTCRVDHRGLTFQQNASSMCVPDQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVTERTVDKSTGKPTLYNVSLVMSDTAGTCY GGGAGTGCATCCGCCCCAACCCTTTTCCCCCTCGTCTCCTGTGAGAATTCCCCGTCGGATACGAGCAGCGTGGCCGTTGGCTGCCTCGCACAGGACTTCCTTCCCGACTCCATCACTTTCTCCTGGAAATACAAGAACAACTCTGACATCAGCAGCACCCGGGGCTTCCCATCAGTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTCCAAGGACGTCATGCAGGGCACAGACGAACACGTGGTGTGCAAAGTCCAGCACCCCAACGGCAAC<span style="color:#FF0000;">AAAGAAAAGAACGTGCCTCTTCCA</span>GTGATTGCTGAGCTGCCTCCCAAAGTGAGCGTCTTCGTCCCACCCCGCGACGGCTTCTTCGGCAACCCCCGCAAGTCCAAGCTCATCTGCCAGGCCACGGGTTTCAGTCCCCGGCAGATTCAGGTGTCCTGGCTGCGCGAGGGGAAGCAGGTGGGGTCTGGCGTCACCACGGACCAGGTGCAGGCTGAGGCCAAAGAGTCTGGGCCCACGACCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCGGCCAGAGCATGTTCACCTGCCGCGTGGATCACAGGGGCCTGACCTTCCAGCAGAATGCGTCCTCCATGTGTGTCCCCGATCAAGACATAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCTCACCAAGTCCACCAAGTTGACCTGCCTGGTCACAGACCTGACCACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTTCAGCGCCGTGGGTGAGGCCAGCATCTGCGAGGATGACTGGAATTCCGGGGAGAGGTTCACGTGCACCGTGACCCACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATCACGTGCCTGGTGACGGGCTTCTCTCCCGCGGACGTCTTCGTGCAGTGGATGCAGAGGGGGCAGCCCTTGTCCCCGGAGAAGTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTCCGAAGAGGAATGGAACACGGGGGAGACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGAGAGGACCGTGGACAAGTCCACCGGTAAACCCACCCTGTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTGA HuIgMRev: TGGAAGAGGCACGTTCTTTTCTTT == Human IgE Constant == A'''STQSP'''SVFPLTRCCKNIPSNATSVTLGCLATGYFPEPVMVTWDTGSLNGTTMTLPATTLTLSGHYATISLLTVSGAWAKQMFTCRVAHTPSSTDWVDNKTFSVCSRDFTPPTVKILQSSCDGGGHFPPTIQLLCLVSGYTPGTINITWLEDGQVMDVDLSTASTTQEGELASTQSELTLSQKHWLSDRTYTCQVTYQGHTFEDSTKKCADSNPRGVSAYLSRPSPFDLFIRKSPTITCLVVDLAPSKGTVNLTWSRASGKPVNHSTRKEEKQRNGTLTVTSTLPVGTRDWIEGETYQCRVTHPHLPRALMRSTTKTSGPRAAPEVYAFATPEWPGSRDKRTLACLIQNFMPEDISVQWLHNEVQLPDARHSTTQPRKTKGSGFFVFSRLEVTRAEWEQKDEFICRAVHEAASPSQTVQRAVSVNPGK == Alignment of Variable Regions == <pre>IgG#4 QVQLQESGPGLVQPSETLSLTCTVSGDSITSNVYYWGWIRQPPGKGLEWIGSIFHSG-IT 59 IgM#1 QVHLQESGPGLVKPSETLSLTCSVSGGSM--NSFYWNWVRQPPGKGLEWIGYIFYSG-IT 57 IgG#2 QVQLVQSGAEVKKPGSSVKVSCKASGDSF--NSLAISWVRQAPGQGLEWMGGIIPKFNRR 58 IgG#3 QMQVVQSGAEVKKPGSSVTVSCKASGGTF--SNYAISWVRQAPGQGLEWMGGIIPLFGTP 58 IgG#1 QVQLVESGGGVVQPGMSHRLSCAASTLIF--DRHALHWVRQAPGAGLEWVAILSYDGTTD 58 IgM#2 EVQLVESGGGLVQPGGSLRLSCSASGFTF--SSYAMHWVRQAPGKGLEYVSAISSNGGST 58 :::: :** : :*. : ::* .* : . *:** ** ***::. : IgG#4 HYTPSLNSRVTISVDTSKNQFSLRLSSATAADTAVYYCARHW---------AGLYFDSWG 110 IgM#1 NYNPSLRSRVTISVDTSKNQFSLKLSSVTTADTALYYCARGG---------SQYIPSYWG 108 IgG#2 DYAQKFQGRVTITADDSASTAYIELSSLTSDDTALYYCARDAE----WAAGSDYFFDYWG 114 IgG#3 TYSQNFQGRVTITADKSTSTAHMELISLRSEDTAVYYCATDRYRQANFDRARVGWFDPWG 118 IgG#1 YYADSVKGRFTVSRDTSKNTVFLQMNGLRPQDTAVYYCARGRALD-DFADYGGYYFDYWG 117 IgM#2 YYADSVKGRFTISRDNSKNTLYLQMSSLRAEDTAVYYCVKDRVLE-WLS--TGYAFDIWG 115 * ....*.*:: * * . :.: . ***:***. . ** IgG#4 QGALVAVSS-------- 119 IgM#1 QGVLVTVSSGSASAPTL 125 IgG#2 QGTLVIVSS-------- 123 IgG#3 QGTLVTVSS-------- 127 IgG#1 QGILVTVSS-------- 126 IgM#2 QGTMVTVSSGSAS---- 128 ** :* *** </pre> == Alignment of Constant Regions == <pre>IGHD APTKAPDVFPIISGCR-HPKDNSPVVLACLITGYHPTS-VTVTWYMGTQS--QPQRTFPE 56 IgM GSASAPTLFPLVSCEN-SPSDTSSVAVGCLAQDFLPDS-ITFSWKYKNNSDISSTRGFPS 58 Ig-hA1 ASPTSPKVFPLSLCST-QPDG--NVVIACLVQGFFPQEPLSVTWSESGQG--VTARNFPP 55 Ig-hA2 ASPTSPKVFPLSLDST-PQDG--NVVVACLVQGFFPQEPLSVTWSESGQN--VTARNFPP 55 IGHE ASTQSPSVFPLTRCCKNIPSNATSVTLGCLATGYFPEP-VMVTWDTGSLN--GTTMTLPA 57 IgG1 ASTKGPSVFPLAPSSKSTS--GGTAALGCLVKDYFPEP-VTVSWNSGALT--SGVHTFPA 55 IgG3 ASTKGPSVFPLAPCSRSTS--GGTAALGCLVKDYFPEP-VTVSWNSGALT--SGVHTFPA 55 IgG2 ASTKGPSVFPLAPCSRSTS--ESTAALGCLVKDYFPEP-VTVSWNSGALT--SGVHTFPA 55 IgG4 ASTKGPSVFPLAPCSRSTS--ESTAALGCLVKDYFPEP-VTVSWNSGALT--SGVHTFPA 55 . .* :**: ..:.** .: * : .:* :* IGHD I-QRRDSYYMTSSQLSTPLQQWRQ---GEYKCVVQHTASKSKKEIF-------------- 98 IgM V--LRGGKYAATSQVLLPSKDVMQGTDEHVVCKVQHPNGNKEK--N--VPLPVIAELPPK 112 Ig-hA1 SQDASGDLYTTSSQLTLPA----------------------------------------- 74 Ig-hA2 SQDASGDLYTTSSQLTLPA----------------------------------------- 74 IGHE TTLTLSGHYATISLLTV-SGAWAK---QMFTCRVAHTPSSTDWVDNKTFSVCSRDFTPPT 113 IgG1 V-LQSSGLYSLSSVVTVPSSSLGT---QTYICNVNHKPSNTKVDKKVE------------ 99 IgG3 V-LQSSGLYSLSSVVTVPSSSLGT---QTYTCNVNHKPSNTKVDKRVELKTPLGDTTHTC 111 IgG2 V-LQSSGLYSLSSVVTVPSSNFGT---QTYTCNVDHKPSNTKVDKTVERK---------- 101 IgG4 V-LQSSGLYSLSSVVTVPSSSLGT---KTYTCNVDHKPSNTKVDKRVESK---------- 101 .. * * : IGHD -----------------------------------RWPESPKAQASSVPTAQPQAEGSLA 123 IgM VS-VFVPPRDGFFGNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKES 171 Ig-hA1 ------------------------------------------------------------ 74 Ig-hA2 ------------------------------------------------------------ 74 IGHE VK-ILQSSCDGGGHFPPTIQLLCLVSGYTPGTINITWLEDGQVMDVDLSTASTTQEGE-- 170 IgG1 ------------------------------------------------------------ 99 IgG3 PRCPEPKSCDTPPPCPR-----CPE----P-----------K------------------ 133 IgG2 ------------------------------------------------------------ 101 IgG4 ------------------------------------------------------------ 101 IGHD KATTAPATTRNT----GRGGEEKKK--EKEKEEQEERETKTPECPSHT------------ 165 IgM GPTTYKVTSTLTIKESDWLGQSMFTCRVDHRGLT--FQQNASSMCVPDQ----------- 218 Ig-hA1 ---------------TQCLAGKSVTCHVKHYTNP--SQDVTVPCPVPSTPPTPSPSTPPT 117 Ig-hA2 ---------------TQCPDGKSVTCHVKHYTNS--SQDVTVPCRVPP------------ 105 IGHE ---LASTQSELTLSQKHWLSDRTYTCQVTYQGHT--FEDSTKKCAD-SNP---------- 214 IgG1 -------------------------------PKSCDKTHTCPPCPAPELL---------- 118 IgG3 ------------------SCDTPPPCPRCPEPKSCDTPPPCPRCPAPELL---------- 165 IgG2 ------------------------------------CCVECPPCPAPPV----------- 114 IgG4 ------------------------------------YGPPCPSCPAPEFL---------- 115 IGHD -----QPLGVYLLTP-AVQDLWLRDKATFTCFVVGSDLKD--AHLTWEVAGKVPTGGVEE 217 IgM ----DTAIRVFAIPP-SFASIFLTKSTKLTCLVTDLTTYD-SVTISWTRQNGEAVK-THT 271 Ig-hA1 PSPSCCHPRLSLHRP-ALEDLLLGSEANLTCTLTGLRDAS-GVTFTWTPSSGKSAV-QGP 174 Ig-hA2 -PPPCCHPRLSLHRP-ALEDLLLGSEANLTCTLTGLRDAS-GATFTWTPSSGKSAV-QGP 161 IGHE -----RGVSAYLSRPSPF-DLFIRKSPTITCLVVDLAPSKGTVNLTWSRASGKPVN-HST 267 IgG1 -----GGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHN-AKT 172 IgG3 -----GGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFKWYVDGVEVHN-AKT 219 IgG2 -----AGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFNWYVDGVEVHN-AKT 168 IgG4 -----GGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHN-AKT 169 * : : .** :.. . . :.* . IGHD GLLERHSNGSQSQHSRLTLPRSLWNAGTSVTCTLNHPSLPPQRLMALREPAAQAPVKLSL 277 IgM NISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDV 331 Ig-hA1 --PERDLCGCYSVSSVLPGCAEPWNHGKTFTCTAAYPESKTPLTATLSKS--GNTFRPEV 230 Ig-hA2 --PERDLCGCYSVSSVLPGCAQPWNHGETFTCTAAHPELKTPLTANITKS--GNTFRPEV 217 IGHE RKEEKQRNGTLTVTSTLPVGTRDWIEGETYQCRVTHPHLPRALMRSTTKTSGP-RAAPEV 326 IgG1 KPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQ-PREPQV 231 IgG3 KPREEQYNSTFRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKTKGQ-PREPQV 278 IgG2 KPREEQFNSTFRVVSVLTVVHQDWLNGKEYKCKVSNKGLPAPIEKTISKTKGQ-PREPQV 227 IgG4 KPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQ-PREPQV 228 * . . . * * * . .: IGHD NLLASSDPP---EAASWLLCEVSGFSPPNILLMWLEDQREVNTSGFAPARPP--PQPRST 332 IgM YLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAP-G 390 Ig-hA1 HLLPPPSEELALNELVTLTCLARGFSPKDVLVRWLQGSQELPREKYLTWASRQEPSQGTT 290 Ig-hA2 HLLPPPSEELALNELVTLTCLARGFSPKDVLVRWLQGSQELPREKYLTWASRQEPSQGTT 277 IGHE YAFATPEWPGS-RDKRTLACLIQNFMPEDISVQWLHNEVQLPDARHSTTQPRKTKG---S 382 IgG1 YTLPPSRDELT-KNQVSLTCLVKGFYPSDIAVEWESNGQ--PENNYKTTPPVLDSD---G 285 IgG3 YTLPPSREEMT-KNQVSLTCLVKGFYPSDIAVEWESSGQ--PENNYNTTPPMLDSD---G 332 IgG2 YTLPPSREEMT-KNQVSLTCLVKGFYPSDISVEWESNGQ--PENNYKTTPPMLDSD---G 281 IgG4 YTLPPSQEEMT-KNQVSLTCLVKGFYPSDIAVEWESNGQ--PENNYKTTPPVLDSD---G 282 : . : * .* * ;:: ;: * . IGHD TFWAWSVLRVPAPPSPQPATYTCVVSHEDSRTLLNASRSLEVSYVT----------DH-- 380 IgM RYFAHSILTVSEEEWNTGETYTCVVAHEALPN-RVTERTVDKSTGKPTLYNVSLVMSDTA 449 Ig-hA1 TFAVTSILRVAAEDWKKGDTFSCMVGHEALPL-AFTQKTIDRLAGKPTHVNVSVVMAEVD 349 Ig-hA2 TYAVTSILRVAAEDWKKGETFSCMVGHEALPL-AFTQKTIDRMAGKPTHINVSVVMAEAD 336 IGHE GFFVFSRLEVTRAEWEQKDEFICRAVHEAASPSQTVQRAVSVNPGK-------------- 428 IgG1 SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHN-HYTQKSLSLSPGK-------------- 330 IgG3 SFFLYSKLTVDKSRWQQGNIFSCSVMHEALHN-RFTQKSLSLSPGK-------------- 377 IgG2 SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHN-HYTQKSLSLSPGK-------------- 326 IgG4 SFFLYSRLTVDKSRWQEGNVFSCSVMHEALHN-HYTQKSLSLSLGK-------------- 327 : * * * : * . ** ..:::. . IGHD GPMK 384 IgM GTCY 453 Ig-hA1 GTCY 353 Ig-hA2 GTCY 340 IGHE ---- 428 IgG1 ---- 330 IgG3 ---- 377 IgG2 ---- 326 IgG4 ---- 327 </pre> [[Category:Antibodies]]
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