Human Antibody Cloning
Human Lambda Vaiable
[edit]L3 VTSYVLTQPPSVSVAPGQTARITCGGNNI---GSKSVHWYQQKPGQAPVLVVYDDSDRPS 57
L1 --QSALTQPRSVSGSPGQSVTISCTGTSSDVGGYNYVSWYQQHPGKAPKLMIYDVSKRPS 58
L2 --QSVLTQPPSVSAAPGQKVTISCSGSSSNIG-NNYVSWYQQLPGTAPKLLIYDNNKRPS 57
. .**** *** :***.. *:* *.. : * **** ** ** *::** ..***
L3 GIPERFSGSNSGNTATLTISRVEAGDEADYYCQVWDSSSDHPT 100
L1 GVPDRFSGSKSGNTASLTISGLQAEDEADYYCCSYAGSYTFH- 100
L2 GIPDRFSGSKSGTSATLGITGLQTGDEADYYCGTWDSSLSA-- 98
*:*:*****:**.:*:* *: ::: ******* : .*
Human Kappa Variable
[edit]K1 DIQMTQSPSSLSASVGDRVTITCRASQDIT-NYVNWFQQRPGQAPKVLIYGASILETGVP 59
K4 DIQMTQSPSSLSASVGDRVTITCRASQSIS-SYLNWYQQKPGKAPKLLIYAASSLQSGVP 59
K2 --ELTQAPGTLSLSPGERATFSCRSSHSIRSRRVRWYQHKPGQAPRLVIHGVSNRASGIS 58
K3 EIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIP 60
:**:*.:** * *:*.*::**:*:.: : *:*::**:**:::*:..* :*:
K1 SRFSGSGSGTDFTFTISSLQPEDIATYYCQQYDTLPLTFGGGTKVDIKR 108
K4 SRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTP------------- 95
K2 DRFSGSGSGTDFTLTITRVEPEDFALYYCQVYGASSYTFGQGTKLERKR 107
K3 DRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSP------------- 96
.************:**: ::***:* **** :
Human Light Chain Kappa Constant
[edit]ACGGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAG
TVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
kappa --TVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQ 58
L1 GQPKANPTVTLFPPSSEELQANKATLVCLISDFYPG---AVTVAWKADGSPVKAGVETTK 57
L2 GQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG---AVTVAWKADSSPVKAGVETTT 57
* * * **** * * * *** *** * * *
kappa DSKDS--TYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC 106
L1 PSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS- 106
L2 PSKSNNKYA-ASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS- 105
** ** * * * * * *** * *
Human Light Chain Lambda 1 Constant
[edit]GGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAG
GQPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADGSPVKAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS
L1 GQPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADGSPVKAGVETTKPSK 60
L2 GQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSK 60
***** *:*************************************.**********.***
L1 QSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS 106
L2 -SNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS 105
*********************************************
L1 GGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAA 60
L2 GGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA 60
************** **** * ******************************** ***
L1 GCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTG 120
L2 GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTG 120
***************** ******** ** ******************** *********
L1 GCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAA 180
L2 GCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAA 180
*************** ********************************* **********
L1 CAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAG 240
L2 CAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAG 240
** ***************************** ************** ************
L1 TCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG 300
L2 TCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG 300
************************************************************
L1 GCCCCTACAGAATGTTCATAG 321
L2 GCCCCTACAGAATGTTCATAG 321
*********************
Human Light Chain Lambda 2 Constant
[edit]GGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAG
GQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS
Human Heavy Chain G1 Constant
[edit]GCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGA
ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT
CPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
to make Fab:
ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT
GS linker aviTag
GGSGSAG GLNDIFEAQKIEWHE-
Human Heavy Chain G2 Constant
[edit]GCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCTCTGACCAGCGGCGTGCACACCTTCCCAGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAACTTCGGCACCCAGACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGTCGAGTGCCCACCGTGCCCAGCACCACCTGTGGCAGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGA
ASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSNFGTQTYTCNVDHKPSNTKVDKTVERKCCVECPPCPAPPVAGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTFRVVSVLTVVHQDWLNGKEYKCKVSNKGLPAPIEKTISKTKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPMLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
Human Heavy Chain G3 Constant
[edit]GCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCAGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACACCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCTCAAAACCCCACTTGGTGACACAACTCACACATGCCCACGGTGCCCAGAGCCCAAATCTTGTGACACACCTCCCCCGTGCCCACGGTGCCCAGAGCCCAAATCTTGTGACACACCTCCCCCATGCCCACGGTGCCCAGAGCCCAAATCTTGTGACACACCTCCCCCGTGCCCAAGGTGCCCAGCACCTGAACTCCTGGGAGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGATACCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGACAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGGAGAGCAGCGGGCAGCCGGAGAACAACTACAACACCACGCCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACATCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCGCTTCACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAGTCCTAG
ASTKGPSVFPLAPCSRSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYTCNVNHKPSNTKVDKRVELKTPLGDTTHTCPRCPEPKSCDTPPPCPRCPEPKSCDTPPPCPRCPEPKSCDTPPPCPRCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFKWYVDGVEVHNAKTKPREEQYNSTFRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKTKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESSGQPENNYNTTPPMLDSDGSFFLYSKLTVDKSRWQQGNIFSCSVMHEALHNRFTQKSLSLSPGK
Human Heavy Chain G4 Constant
[edit]GCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCCGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACGAAGACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGTCCAAATATGGTCCCCCATGCCCATCATGCCCAGCACCTGAGTTCCTGGGGGGACCATCAGTCTTCCTGTTCCCCCCAAAACCCAAGGACACTCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGTGGATGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTTCAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAGCCACAGGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAGGCTCACCGTGGACAAGAGCAGGTGGCAGGAGGGGAATGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACACAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGA
ASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYGPPCPSCPAPEFLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLSLSPGK
Human IgM Constant
[edit]GSASAPTLFPLVSCENSPSDTSSVAVGCLAQDFLPDSITFSWKYKNNSDISSTRGFPSVLRGGKYAATSQVLLPSKDVMQGTDEHVVCKVQHPNGNKEKNVPLPVIAELPPKVSVFVPPRDGFFGNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKESGPTTYKVTSTLTIKESDWLGQSMFTCRVDHRGLTFQQNASSMCVPDQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVTERTVDKSTGKPTLYNVSLVMSDTAGTCY
GGGAGTGCATCCGCCCCAACCCTTTTCCCCCTCGTCTCCTGTGAGAATTCCCCGTCGGATACGAGCAGCGTGGCCGTTGGCTGCCTCGCACAGGACTTCCTTCCCGACTCCATCACTTTCTCCTGGAAATACAAGAACAACTCTGACATCAGCAGCACCCGGGGCTTCCCATCAGTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTCCAAGGACGTCATGCAGGGCACAGACGAACACGTGGTGTGCAAAGTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGATTGCTGAGCTGCCTCCCAAAGTGAGCGTCTTCGTCCCACCCCGCGACGGCTTCTTCGGCAACCCCCGCAAGTCCAAGCTCATCTGCCAGGCCACGGGTTTCAGTCCCCGGCAGATTCAGGTGTCCTGGCTGCGCGAGGGGAAGCAGGTGGGGTCTGGCGTCACCACGGACCAGGTGCAGGCTGAGGCCAAAGAGTCTGGGCCCACGACCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCGGCCAGAGCATGTTCACCTGCCGCGTGGATCACAGGGGCCTGACCTTCCAGCAGAATGCGTCCTCCATGTGTGTCCCCGATCAAGACATAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCTCACCAAGTCCACCAAGTTGACCTGCCTGGTCACAGACCTGACCACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTTCAGCGCCGTGGGTGAGGCCAGCATCTGCGAGGATGACTGGAATTCCGGGGAGAGGTTCACGTGCACCGTGACCCACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATCACGTGCCTGGTGACGGGCTTCTCTCCCGCGGACGTCTTCGTGCAGTGGATGCAGAGGGGGCAGCCCTTGTCCCCGGAGAAGTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTCCGAAGAGGAATGGAACACGGGGGAGACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGAGAGGACCGTGGACAAGTCCACCGGTAAACCCACCCTGTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTGA
HuIgMRev: TGGAAGAGGCACGTTCTTTTCTTT
Human IgE Constant
[edit]ASTQSPSVFPLTRCCKNIPSNATSVTLGCLATGYFPEPVMVTWDTGSLNGTTMTLPATTLTLSGHYATISLLTVSGAWAKQMFTCRVAHTPSSTDWVDNKTFSVCSRDFTPPTVKILQSSCDGGGHFPPTIQLLCLVSGYTPGTINITWLEDGQVMDVDLSTASTTQEGELASTQSELTLSQKHWLSDRTYTCQVTYQGHTFEDSTKKCADSNPRGVSAYLSRPSPFDLFIRKSPTITCLVVDLAPSKGTVNLTWSRASGKPVNHSTRKEEKQRNGTLTVTSTLPVGTRDWIEGETYQCRVTHPHLPRALMRSTTKTSGPRAAPEVYAFATPEWPGSRDKRTLACLIQNFMPEDISVQWLHNEVQLPDARHSTTQPRKTKGSGFFVFSRLEVTRAEWEQKDEFICRAVHEAASPSQTVQRAVSVNPGK
Alignment of Variable Regions
[edit]IgG#4 QVQLQESGPGLVQPSETLSLTCTVSGDSITSNVYYWGWIRQPPGKGLEWIGSIFHSG-IT 59
IgM#1 QVHLQESGPGLVKPSETLSLTCSVSGGSM--NSFYWNWVRQPPGKGLEWIGYIFYSG-IT 57
IgG#2 QVQLVQSGAEVKKPGSSVKVSCKASGDSF--NSLAISWVRQAPGQGLEWMGGIIPKFNRR 58
IgG#3 QMQVVQSGAEVKKPGSSVTVSCKASGGTF--SNYAISWVRQAPGQGLEWMGGIIPLFGTP 58
IgG#1 QVQLVESGGGVVQPGMSHRLSCAASTLIF--DRHALHWVRQAPGAGLEWVAILSYDGTTD 58
IgM#2 EVQLVESGGGLVQPGGSLRLSCSASGFTF--SSYAMHWVRQAPGKGLEYVSAISSNGGST 58
:::: :** : :*. : ::* .* : . *:** ** ***::. :
IgG#4 HYTPSLNSRVTISVDTSKNQFSLRLSSATAADTAVYYCARHW---------AGLYFDSWG 110
IgM#1 NYNPSLRSRVTISVDTSKNQFSLKLSSVTTADTALYYCARGG---------SQYIPSYWG 108
IgG#2 DYAQKFQGRVTITADDSASTAYIELSSLTSDDTALYYCARDAE----WAAGSDYFFDYWG 114
IgG#3 TYSQNFQGRVTITADKSTSTAHMELISLRSEDTAVYYCATDRYRQANFDRARVGWFDPWG 118
IgG#1 YYADSVKGRFTVSRDTSKNTVFLQMNGLRPQDTAVYYCARGRALD-DFADYGGYYFDYWG 117
IgM#2 YYADSVKGRFTISRDNSKNTLYLQMSSLRAEDTAVYYCVKDRVLE-WLS--TGYAFDIWG 115
* ....*.*:: * * . :.: . ***:***. . **
IgG#4 QGALVAVSS-------- 119
IgM#1 QGVLVTVSSGSASAPTL 125
IgG#2 QGTLVIVSS-------- 123
IgG#3 QGTLVTVSS-------- 127
IgG#1 QGILVTVSS-------- 126
IgM#2 QGTMVTVSSGSAS---- 128
** :* ***
Alignment of Constant Regions
[edit]IGHD APTKAPDVFPIISGCR-HPKDNSPVVLACLITGYHPTS-VTVTWYMGTQS--QPQRTFPE 56
IgM GSASAPTLFPLVSCEN-SPSDTSSVAVGCLAQDFLPDS-ITFSWKYKNNSDISSTRGFPS 58
Ig-hA1 ASPTSPKVFPLSLCST-QPDG--NVVIACLVQGFFPQEPLSVTWSESGQG--VTARNFPP 55
Ig-hA2 ASPTSPKVFPLSLDST-PQDG--NVVVACLVQGFFPQEPLSVTWSESGQN--VTARNFPP 55
IGHE ASTQSPSVFPLTRCCKNIPSNATSVTLGCLATGYFPEP-VMVTWDTGSLN--GTTMTLPA 57
IgG1 ASTKGPSVFPLAPSSKSTS--GGTAALGCLVKDYFPEP-VTVSWNSGALT--SGVHTFPA 55
IgG3 ASTKGPSVFPLAPCSRSTS--GGTAALGCLVKDYFPEP-VTVSWNSGALT--SGVHTFPA 55
IgG2 ASTKGPSVFPLAPCSRSTS--ESTAALGCLVKDYFPEP-VTVSWNSGALT--SGVHTFPA 55
IgG4 ASTKGPSVFPLAPCSRSTS--ESTAALGCLVKDYFPEP-VTVSWNSGALT--SGVHTFPA 55
. .* :**: ..:.** .: * : .:* :*
IGHD I-QRRDSYYMTSSQLSTPLQQWRQ---GEYKCVVQHTASKSKKEIF-------------- 98
IgM V--LRGGKYAATSQVLLPSKDVMQGTDEHVVCKVQHPNGNKEK--N--VPLPVIAELPPK 112
Ig-hA1 SQDASGDLYTTSSQLTLPA----------------------------------------- 74
Ig-hA2 SQDASGDLYTTSSQLTLPA----------------------------------------- 74
IGHE TTLTLSGHYATISLLTV-SGAWAK---QMFTCRVAHTPSSTDWVDNKTFSVCSRDFTPPT 113
IgG1 V-LQSSGLYSLSSVVTVPSSSLGT---QTYICNVNHKPSNTKVDKKVE------------ 99
IgG3 V-LQSSGLYSLSSVVTVPSSSLGT---QTYTCNVNHKPSNTKVDKRVELKTPLGDTTHTC 111
IgG2 V-LQSSGLYSLSSVVTVPSSNFGT---QTYTCNVDHKPSNTKVDKTVERK---------- 101
IgG4 V-LQSSGLYSLSSVVTVPSSSLGT---KTYTCNVDHKPSNTKVDKRVESK---------- 101
.. * * :
IGHD -----------------------------------RWPESPKAQASSVPTAQPQAEGSLA 123
IgM VS-VFVPPRDGFFGNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKES 171
Ig-hA1 ------------------------------------------------------------ 74
Ig-hA2 ------------------------------------------------------------ 74
IGHE VK-ILQSSCDGGGHFPPTIQLLCLVSGYTPGTINITWLEDGQVMDVDLSTASTTQEGE-- 170
IgG1 ------------------------------------------------------------ 99
IgG3 PRCPEPKSCDTPPPCPR-----CPE----P-----------K------------------ 133
IgG2 ------------------------------------------------------------ 101
IgG4 ------------------------------------------------------------ 101
IGHD KATTAPATTRNT----GRGGEEKKK--EKEKEEQEERETKTPECPSHT------------ 165
IgM GPTTYKVTSTLTIKESDWLGQSMFTCRVDHRGLT--FQQNASSMCVPDQ----------- 218
Ig-hA1 ---------------TQCLAGKSVTCHVKHYTNP--SQDVTVPCPVPSTPPTPSPSTPPT 117
Ig-hA2 ---------------TQCPDGKSVTCHVKHYTNS--SQDVTVPCRVPP------------ 105
IGHE ---LASTQSELTLSQKHWLSDRTYTCQVTYQGHT--FEDSTKKCAD-SNP---------- 214
IgG1 -------------------------------PKSCDKTHTCPPCPAPELL---------- 118
IgG3 ------------------SCDTPPPCPRCPEPKSCDTPPPCPRCPAPELL---------- 165
IgG2 ------------------------------------CCVECPPCPAPPV----------- 114
IgG4 ------------------------------------YGPPCPSCPAPEFL---------- 115
IGHD -----QPLGVYLLTP-AVQDLWLRDKATFTCFVVGSDLKD--AHLTWEVAGKVPTGGVEE 217
IgM ----DTAIRVFAIPP-SFASIFLTKSTKLTCLVTDLTTYD-SVTISWTRQNGEAVK-THT 271
Ig-hA1 PSPSCCHPRLSLHRP-ALEDLLLGSEANLTCTLTGLRDAS-GVTFTWTPSSGKSAV-QGP 174
Ig-hA2 -PPPCCHPRLSLHRP-ALEDLLLGSEANLTCTLTGLRDAS-GATFTWTPSSGKSAV-QGP 161
IGHE -----RGVSAYLSRPSPF-DLFIRKSPTITCLVVDLAPSKGTVNLTWSRASGKPVN-HST 267
IgG1 -----GGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHN-AKT 172
IgG3 -----GGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFKWYVDGVEVHN-AKT 219
IgG2 -----AGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFNWYVDGVEVHN-AKT 168
IgG4 -----GGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHN-AKT 169
* : : .** :.. . . :.* .
IGHD GLLERHSNGSQSQHSRLTLPRSLWNAGTSVTCTLNHPSLPPQRLMALREPAAQAPVKLSL 277
IgM NISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDV 331
Ig-hA1 --PERDLCGCYSVSSVLPGCAEPWNHGKTFTCTAAYPESKTPLTATLSKS--GNTFRPEV 230
Ig-hA2 --PERDLCGCYSVSSVLPGCAQPWNHGETFTCTAAHPELKTPLTANITKS--GNTFRPEV 217
IGHE RKEEKQRNGTLTVTSTLPVGTRDWIEGETYQCRVTHPHLPRALMRSTTKTSGP-RAAPEV 326
IgG1 KPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQ-PREPQV 231
IgG3 KPREEQYNSTFRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKTKGQ-PREPQV 278
IgG2 KPREEQFNSTFRVVSVLTVVHQDWLNGKEYKCKVSNKGLPAPIEKTISKTKGQ-PREPQV 227
IgG4 KPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQ-PREPQV 228
* . . . * * * . .:
IGHD NLLASSDPP---EAASWLLCEVSGFSPPNILLMWLEDQREVNTSGFAPARPP--PQPRST 332
IgM YLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAP-G 390
Ig-hA1 HLLPPPSEELALNELVTLTCLARGFSPKDVLVRWLQGSQELPREKYLTWASRQEPSQGTT 290
Ig-hA2 HLLPPPSEELALNELVTLTCLARGFSPKDVLVRWLQGSQELPREKYLTWASRQEPSQGTT 277
IGHE YAFATPEWPGS-RDKRTLACLIQNFMPEDISVQWLHNEVQLPDARHSTTQPRKTKG---S 382
IgG1 YTLPPSRDELT-KNQVSLTCLVKGFYPSDIAVEWESNGQ--PENNYKTTPPVLDSD---G 285
IgG3 YTLPPSREEMT-KNQVSLTCLVKGFYPSDIAVEWESSGQ--PENNYNTTPPMLDSD---G 332
IgG2 YTLPPSREEMT-KNQVSLTCLVKGFYPSDISVEWESNGQ--PENNYKTTPPMLDSD---G 281
IgG4 YTLPPSQEEMT-KNQVSLTCLVKGFYPSDIAVEWESNGQ--PENNYKTTPPVLDSD---G 282
: . : * .* * ;:: ;: * .
IGHD TFWAWSVLRVPAPPSPQPATYTCVVSHEDSRTLLNASRSLEVSYVT----------DH-- 380
IgM RYFAHSILTVSEEEWNTGETYTCVVAHEALPN-RVTERTVDKSTGKPTLYNVSLVMSDTA 449
Ig-hA1 TFAVTSILRVAAEDWKKGDTFSCMVGHEALPL-AFTQKTIDRLAGKPTHVNVSVVMAEVD 349
Ig-hA2 TYAVTSILRVAAEDWKKGETFSCMVGHEALPL-AFTQKTIDRMAGKPTHINVSVVMAEAD 336
IGHE GFFVFSRLEVTRAEWEQKDEFICRAVHEAASPSQTVQRAVSVNPGK-------------- 428
IgG1 SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHN-HYTQKSLSLSPGK-------------- 330
IgG3 SFFLYSKLTVDKSRWQQGNIFSCSVMHEALHN-RFTQKSLSLSPGK-------------- 377
IgG2 SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHN-HYTQKSLSLSPGK-------------- 326
IgG4 SFFLYSRLTVDKSRWQEGNVFSCSVMHEALHN-HYTQKSLSLSLGK-------------- 327
: * * * : * . ** ..:::. .
IGHD GPMK 384
IgM GTCY 453
Ig-hA1 GTCY 353
Ig-hA2 GTCY 340
IGHE ---- 428
IgG1 ---- 330
IgG3 ---- 377
IgG2 ---- 326
IgG4 ---- 327