Editing MS 5 15 2017

run SDS-PAGE gel '''<span style="color:#FF0000">1518 T3 Ligase/1542 sso7d ToughTaq</span>''' 05/15/2017

{| style="width: 500px" cellspacing="1" cellpadding="1" border="1"
|-
| <br/>
| rowspan="1" colspan="5" | 1518 T3 Ligase 41.6<br/>
| <br/>
| rowspan="1" colspan="6" | 1542 sso7d Native ToughTaq 72.0<br/>
|-
| Number<br/>
| 1<br/>
| 2<br/>
| 3<br/>
| 4<br/>
| 5<br/>
| 6<br/>
| 7<br/>
| 8<br/>
| 9<br/>
| 10<br/>
| 11<br/>
| 12<br/>
|-
| sample<br/>
| FT1<br/>
| <span style="color:#000000">23</span><br/>
| 25<br/>
| 29<br/>
| FT2<br/>
| MW<br/>
| FT1<br/>
| 25<br/>
| 26<br/>
| 29<br/>
| 30<br/>
| FT2<br/>
|-
| uL<br/>
| 5<br/>
| 2.8<br/>
| 1.2<br/>
| 0.4<br/>
| 15<br/>
| 10<br/>
| 5<br/>
| 0.6<br/>
| 0.5<br/>
| 0.5<br/>
| 0.4<br/>
| 15<br/>
|-
| ddH<sub>2</sub>O<br/>
| 10<br/>
| 10<br/>
| 15<br/>
| 15<br/>
| \<br/>
| \<br/>
| 10<br/>
| 15<br/>
| 15<br/>
| 15<br/>
| 15<br/>
| \<br/>
|}

[[File:Protein Expression SDS-PAGE 119-1518 T3 DNA Ligase and 120-1542 Native ToughTaq QHP 5 15 2017.jpg|RTENOTITLE]]&nbsp;



Protein Expression Screening of 483 Tth Polymerase /1464 T7 DNA Ligase / 1946 TdT bovine / 1497 ToughTaq

{| cellspacing="1" cellpadding="1" border="1"
|-
| Number<br/>
| 1<br/>
| 2<br/>
| 3<br/>
| 4<br/>
| 5<br/>
| 6<br/>
| 7<br/>
| 8<br/>
| 9<br/>
| 10<br/>
| 11<br/>
| 12<br/>
|-
| sample<br/>
| 
483

1

| 
<span style="color:#000000">1464</span>

<span style="color:#000000">1</span>

| 
1946

1

| 
1497

1

| MW<br/>
| 
483

2

| 
1464

2

| 
1946

2

| 
1497

2

| 
1518

28

| 
1518

30

| 
1542

31

|-
| uL<br/>
| 15<br/>
| 15<br/>
| 15<br/>
| 15<br/>
| 10<br/>
| 15<br/>
| 15<br/>
| 15<br/>
| 15<br/>
| 1.3<br/>
| 0.8<br/>
| 0.8<br/>
|-
| ddH<sub>2</sub>O<br/>
| \<br/>
| \<br/>
| \<br/>
| \<br/>
| \<br/>
| \<br/>
| \<br/>
| \<br/>
| \<br/>
| 15<br/>
| 15<br/>
| 15<br/>
|}

1: induced with 0.5 mM IPTG

2: without induce

[[File:Protein Screening 483 1464 1946 1497 1518(28) 1518(30) 1542(31) 05 15 2017.jpg|RTENOTITLE]]

Conclusion: 483 1464 1946 1497 need to do from plasmid transformation. 1518(29, 30) and 1542( 29,30,31) can forward to the next step.
[[Category:My ELN]]