MS 5 15 2017
Jump to navigation
Jump to search
run SDS-PAGE gel 1518 T3 Ligase/1542 sso7d ToughTaq 05/15/2017
| 1518 T3 Ligase 41.6 |
1542 sso7d Native ToughTaq 72.0 | |||||||||||
| Number |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
| sample |
FT1 |
23 |
25 |
29 |
FT2 |
MW |
FT1 |
25 |
26 |
29 |
30 |
FT2 |
| uL |
5 |
2.8 |
1.2 |
0.4 |
15 |
10 |
5 |
0.6 |
0.5 |
0.5 |
0.4 |
15 |
| ddH2O |
10 |
10 |
15 |
15 |
\ |
\ |
10 |
15 |
15 |
15 |
15 |
\ |
Protein Expression Screening of 483 Tth Polymerase /1464 T7 DNA Ligase / 1946 TdT bovine / 1497 ToughTaq
| Number |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
| sample |
483 1 |
1464 1 |
1946 1 |
1497 1 |
MW |
483 2 |
1464 2 |
1946 2 |
1497 2 |
1518 28 |
1518 30 |
1542 31 |
| uL |
15 |
15 |
15 |
15 |
10 |
15 |
15 |
15 |
15 |
1.3 |
0.8 |
0.8 |
| ddH2O |
\ |
\ |
\ |
\ |
\ |
\ |
\ |
\ |
\ |
15 |
15 |
15 |
1: induced with 0.5 mM IPTG
2: without induce
_1518(30)_1542(31)_05_15_2017.jpg)
Conclusion: 483 1464 1946 1497 need to do from plasmid transformation. 1518(29, 30) and 1542( 29,30,31) can forward to the next step.